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Alkali-enzymolysis method for extracting chondroitin sulfate sodium

A technology of chondroitin sulfate sodium and cartilage, which is applied in the field of biochemical pharmacy, can solve the problems of affecting the yield and quality, taking a long time, and easy dissociation of amino groups, so as to improve production efficiency, improve production quality and shorten production cycle Effect

Active Publication Date: 2014-03-26
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above concentrated extraction methods, although the yield can reach a very high level, there are still big problems in the production methods, such as the removal of acidic protein in the process of salt solution to adjust the pH value of 1-2, which takes a long time when the product volume is large. Long, the amino group in sodium chondroitin sulfate is easily dissociated, thus affecting the yield and quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Soaking: 100 kg of pretreated white and clean animal cartilage blocks are placed in a reaction kettle, and an appropriate amount of water is added, and soaked for 5 hours;

[0017] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage at a ratio of 1:1 to the weight of the cartilage, and add alkali tablets to 4% of the weight of the cartilage, and stir for 3 hours;

[0018] (3) Enzymolysis: adjust the pH of the above alkaline solution to 8.0, heat to 45°C, add porcine pancreas according to 12% of the cartilage weight, add sodium chloride, and keep warm at 45°C for 3 hours;

[0019] (4) Reduction: adjust the pH of the above enzymolysis solution to 6.5, heat to 65°C, add 1% sodium dithionite based on the cartilage weight, stir for 15 minutes, and filter after standing for 20 minutes;

[0020] (5) Precipitation: cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°C at 20°C, and precipita...

Embodiment 2

[0024] (1) Soaking: 100kg of pretreated white and clean animal cartilage blocks are placed in a reaction kettle, and an appropriate amount of water is added, and soaked for 6 hours;

[0025] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage according to the weight of the cartilage at a ratio of 1:1, and add alkali tablets according to 5% of the weight of the cartilage, and stir for 4 hours;

[0026] (3) Enzymolysis: adjust the pH of the above alkaline solution to 8.5, heat to 50°C, add porcine pancreas according to 15% of the cartilage weight, add sodium chloride, and keep warm at 50°C for 4 hours;

[0027] (4) Reduction: adjust the pH of the above enzymatic solution to 7.0, heat to 65°C, add 1% sodium dithionite based on the weight of the cartilage, stir for 15 minutes, and filter after standing for 20 minutes;

[0028] (5) Precipitation: cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°...

Embodiment 3

[0032] (1) Soaking: 100kg of pretreated white and clean animal cartilage blocks are placed in a reaction kettle, and an appropriate amount of water is added, and soaked for 6 hours;

[0033] (2) Alkaline hydrolysis: Add water to the fully soaked animal cartilage at a ratio of 1:1 of the weight of the cartilage, and add alkali tablets according to 6% of the weight of the cartilage, and stir for 4 hours;

[0034] (3) Enzymolysis: adjust the pH of the above alkaline solution to 8.5, heat to 48°C, add porcine pancreas according to 14% of the cartilage weight, add sodium chloride, and keep warm at 50°C for 4 hours;

[0035] (4) Reduction: adjust the pH of the above enzymatic solution to 7.0, heat to 65°C, add 1% sodium dithionite based on the weight of the cartilage, stir for 15 minutes, and filter after standing for 20 minutes;

[0036] (5) Precipitation: cool down the filtrate obtained in the previous step below 30°C, add ethanol, make the precipitation degree reach 60°C at 20°C,...

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Abstract

The invention discloses a novel method for extracting chondroitin sulfate sodium, namely an alkali-enzymolysis method for extracting chondroitin sulfate sodium, and mainly solves the problems of complicated production technology processes, larger alkali, acid or salt dosage, higher production cost, longer extraction period, unstable product quality, lower yield and the like for similar products at present. The product mainly adopts animal cartilage as a raw material, and chondroitin sulfate sodium can be extracted from the animal cartilage after alkaline hydrolysis and further enzymolysis; and the color of the product is pure white, the powder is loosened, the yield can be higher than 30%, moreover, the purity is high, and the product meets the oral quality standard. The alkali-enzymolysis method for extracting chondroitin sulfate sodium has the characteristics of simple production process, short period, low cost, simplicity, convenience and easiness in operation, capacities of reducing environment pollution and facilitating environmental protection, and the like.

Description

technical field [0001] The invention relates to the field of biochemical pharmacy, in particular to a production process of sodium chondroitin sulfate which is directly extracted from animal cartilage and has good effects on cardiovascular and cerebrovascular diseases. Background technique [0002] Chondroitin sulfate sodium, English name Chondroit in sulfate sodium, is an acidic mucopolysaccharide extracted from animal cartilage, mostly present in animal cartilage, laryngeal bone, nasal bone (41%), bovine, equine septum and trachea, its molecular structure It is a polysaccharide chain composed of repeating disaccharide units formed by β-D-glucuronic acid and N-acetylgalactosamine through β1→3 glycosidic bonds. [0003] Sodium chondroitin sulfate is mainly used in the treatment of rheumatism and rheumatoid diseases. It also has anticoagulant, anticancer, antithrombotic, and blood lipid-lowering effects. It can be used to treat symptoms such as headache, migraine, coronary he...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069
Inventor 刘乃山陆玉梅迟培升夏衬来
Owner QINGDAO JIULONG BIO PHARMA
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