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Method for reducing residual ethanol of chondroitin sulfate sodium

A chondroitin sulfate sodium and ethanol technology is applied in the field of reducing chondroitin sulfate sodium ethanol residue, which can solve problems such as increasing process time and prolonging drying time, achieves small environmental pollution, reduces ethanol residue, and improves content and yield Effect

Inactive Publication Date: 2019-06-25
QINGDAO JIULONG BIO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to reduce the residue of ethanol, the final drying time is prolonged and the process time is increased

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Reduction: adjust 500 L of the dissociated solution after alkaline hydrolysis and enzymatic hydrolysis, adjust the pH to 7.0, raise the temperature to 65°C, add 4 kg of sodium pyrosulfate, stir for 25 minutes, let stand for 25 minutes, and filter;

[0017] 2. Ethanol precipitation: Keep the temperature at 65°C, keep the pH at 7.0, add ethanol while stirring, so that the concentration of ethanol is 70°C, and let stand for 6 hours. The precipitate was separated by centrifugation.

[0018] 3. Re-precipitation with ethanol: Add 670 L of purified water to dissolve the precipitate, and raise the temperature to 65°C. Add 7 kg of sodium chloride, and add ethanol while stirring, so that the degree of ethanol precipitation reaches 60 degrees, and let it stand for 5 hours.

[0019] 4. Dehydration: suck out the supernatant, add absolute ethanol to the sediment while stirring, so that the alcohol content reaches 95 degrees, and let it stand for more than 24 hours. The supernata...

Embodiment 2

[0021] 1. Reduction: adjust 50 L of the dissociated solution after alkaline hydrolysis and enzymatic hydrolysis, adjust the pH to 7.0, raise the temperature to 65°C, add 400 g of sodium pyrosulfate, stir for 35 minutes, let stand for 35 minutes, and then filter;

[0022] 2. Ethanol precipitation: keep the temperature at 60°C, keep the pH at 6.5, add ethanol while stirring to make the ethanol concentration 70°C, and let stand for 6 hours. The precipitate was separated by centrifugation.

[0023] 3. Ethanol re-precipitation: add 67 L of purified water to dissolve the precipitate, and raise the temperature to 60°C. Add 700 g of sodium chloride, and add ethanol while stirring, so that the degree of ethanol precipitation reaches 60 degrees, and let it stand for 5 hours.

[0024] 4. Dehydration: suck out the supernatant, add absolute ethanol to the sediment while stirring, so that the alcohol content reaches 95 degrees, and let it stand for more than 24 hours. The supernatant was ...

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Abstract

The present invention relates to a method for reducing the residual ethanol of chondroitin sulfate sodium, wherein chondroitin sulfate sodium is a mucopolysaccharide substance extracted from pig nasalbone and other animal cartilages, and mainly precipitation purification and dewatering is performed with ethanol. According to the present invention, mainly thermal precipitation and dewatering wit ethanol is performed to reduce the ethanol content in chondroitin sulfate and reduce the residual ethanol; and the method has characteristics of simple process, mild condition, safe production, low residue ethanol content, improved chondroitin sulfate yield and improved purity of chondroitin sulfate, and is the process suitable for large-scale industrial production.

Description

technical field [0001] The field of the invention belongs to the field of biopharmaceuticals, and in particular relates to a method for reducing chondroitin sulfate sodium ethanol residue. Background technique [0002] Sodium chondroitin sulfate is an acidic mucopolysaccharide extracted from animal cartilage tissue, which belongs to glycosaminoglycan. Sodium chondroitin sulfate is mainly used in the treatment of rheumatism and rheumatoid diseases. It also has anticoagulant, anticancer, antithrombotic, and blood lipid-lowering effects. It can be used to treat symptoms such as headache, migraine, coronary heart disease, and angina. [0003] In the current domestic technology of separating and purifying sodium chondroitin sulfate, a large number of organic solvents such as ethanol are used for precipitation and dehydration. In order to reduce the residue of ethanol, the final drying time is prolonged and the process time is increased. The method has the advantages of simple p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 刘乃山迟培升刘莉
Owner QINGDAO JIULONG BIO PHARMA
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