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Method of extracting chondroitin 6-sulfate sodium salt from animal cartilage by solid-phase alkali hydrolysis

A technology of chondroitin sulfate sodium and alkaline hydrolysis, which is applied in the field of chondroitin sulfate sodium, can solve the problems of long production cycle, cumbersome process, and large waste liquid pollution, and achieve the effect of short production cycle, simple process and efficient extraction

Active Publication Date: 2017-06-09
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that these methods have the disadvantages of cumbersome process, long production cycle, and large waste liquid pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Clean the pig nose cartilage and peel off the remaining minced meat and fascia, add water until the cartilage is just submerged, heat to boil and keep it for 30 minutes, drain the water while it is hot, dry the cartilage at 110°C for 12 hours, crush it, and pass through a 200-mesh sieve to obtain Pig nose cartilage powder.

[0022] Weigh 50g pig nose cartilage powder, 5gNaOH, 10gNaCl, mix and grind the three in a mortar for 40min, add 200ml water to the solid mixture, stir the system for 30min, adjust the pH of the system to 7.0 with 1M HCl, filter; Under good stirring, add 2.5 times the volume of ethanol dropwise to the filtrate. After the dropwise addition, let it stand and age for 8 hours, filter, wash the filter cake twice with a small amount of ethanol, and dry it in vacuum at 60°C to obtain white chondroitin sulfate sodium powder The body is 6.4g, and the HPLC purity is 90%.

Embodiment 2

[0024] Clean the pig nose cartilage and peel off the remaining minced meat and fascia, add ethanol until the cartilage is just submerged, soak for 3 hours, drain the ethanol, dry the cartilage at 110°C for 12 hours, pulverize, and pass through a 200-mesh sieve to obtain pig nose cartilage powder.

[0025] Weigh 50g pig nose cartilage powder, 25gNaOH, 20gNaCl, 70g Al with a particle size of 100-200mesh 2 o 3 , mix and grind the four components in a mortar for 30 minutes, add 350ml of water to the solid mixture, stir the system for 30 minutes, adjust the pH of the system to 7.0 with 1M HCl, and filter; under good stirring, pour into the filtrate Add 2.5 times the volume of ethanol dropwise, after the dropwise addition, let stand and age for 8hr, filter, wash the filter cake twice with a small amount of ethanol, and dry it in vacuum at 60°C to obtain 9.2g of white and loose sodium chondroitin sulfate powder, HPLC purity 96%.

Embodiment 3

[0027] Clean the pig nose cartilage and peel off the remaining minced meat and fascia, add water until the cartilage is just submerged, heat to boil and keep it for 30 minutes, drain the water while it is hot, dry the cartilage at 110°C for 12 hours, crush it, and pass through a 200-mesh sieve to obtain Pig nose cartilage powder.

[0028] Weigh 50g pig nose cartilage powder, 25gNaOH, 20gNaCl, 40g Al with a particle size of 100-200mesh 2 o 3 , mix and grind the four components in a mortar for 30 minutes, add 400ml of water to the solid mixture, stir the system for 30 minutes, adjust the pH of the system to 6.5 with 1M HCl, and filter; under good stirring, pour into the filtrate Add 2.5 times the volume of ethanol dropwise, after the dropwise addition, let stand and age for 8hr, filter, wash the filter cake twice with a small amount of ethanol, and dry it in vacuum at 60°C to obtain 9.5g of white loose sodium chondroitin sulfate powder, HPLC purity 98%.

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Abstract

The invention discloses a method of extracting chondroitin 6-sulfate sodium salt from animal cartilage by solid-phase alkali hydrolysis; the method replaces existing common liquid-phase alkali-enzymatic hydrolysis by solid-phase alkali hydrolysis; at room temperature, animal cartilage powder is mixed with NaOH and NaCl, reaction is triggered under the action of mechanical force, and the extraction and separation process of chondroitin 6-sulfate sodium salt in solid phase is finished efficiently by making use of the features of the solid phase reaction, such as high selectivity, high yield and good process simplicity; the method has the advantages of good process simplicity, high extraction rate, short production cycle, low solvent usage, and low emission of liquid waste.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a method for extracting sodium chondroitin sulfate from animal cartilage by using a solid-phase alkali hydrolysis method. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide, which is mainly used clinically to treat neuralgia, arthritis, tinnitus, promote ulcer healing and prevent hyperlipidemia. [0003] Chondroitin sulfate widely exists in the cartilage tissue of animals and is currently produced by extraction. Since the extraction process is mostly carried out in the presence of NaOH, the final product is usually obtained in the form of sodium chondroitin sulfate. Chondroitin sulfate in animal cartilage is usually linked to protein molecules by covalent bonds to form proteoglycans. Therefore, when extracting chondroitin sulfate, it must first be dissociated from protein molecules, and then the impurity protein molecules are denatured. Separate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0069
Inventor 刘金涛李青
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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