Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Test strip and kit for quantitatively detecting GDF-15

A technology for quantitative detection of GDF-15, applied in the field of molecular biology, can solve difficult problems and achieve the effects of simplified components, high precision, and good linear range

Pending Publication Date: 2022-02-18
HUNAN YONGHE YANGGUANG SCI & TECH
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the difficulty in developing the performance of GDF-15 detection in immunochromatographic reagents, there are no reports in the prior art about the use of time-resolved fluorescence immunoassays to determine the content of GDF-15 in samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Test strip and kit for quantitatively detecting GDF-15
  • Test strip and kit for quantitatively detecting GDF-15
  • Test strip and kit for quantitatively detecting GDF-15

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0030] In the present invention, the preparation method of fluorescent microsphere-labeled mouse anti-human GDF-15 monoclonal antibody preferably includes the following steps: mixing the fluorescent microsphere solution with boric acid-borax buffer, vortexing, ultrasonication, and centrifugation to obtain the first Precipitate; activate and centrifuge the first precipitate with EDC solution to obtain the second precipitate; mix and react the second precipitate with mouse anti-human GDF-15 monoclonal antibody solution and boric acid-borax buffer solution to obtain the reactant, centrifuge, The third precipitate was obtained; the third precipitate was blocked overnight with a blocking solution to obtain a mouse anti-human GDF-15 monoclonal antibody.

[0031] In the present invention, the concentration of the fluorescent microsphere solution is preferably 5-10 mg / mL; the concentration of the boric acid-borax buffer solution is preferably 0.01-0.02 mol / L, and the pH value is prefer...

Embodiment 1

[0049] Preparation of sample pads:

[0050] Sample pad pretreatment: Spray the sample pad treatment solution (0.1M Tris-HCl buffer (pH8.00) solution containing 0.02% anti-erythrocyte antibody and 0.01% HBR blocking agent) evenly on the On the glass fiber, dry at 37°C for 4 hours to obtain the treated glass fiber.

[0051] Preparation of fluorescent microsphere-labeled mouse anti-human GDF-15 monoclonal antibody: Add 100 μL of time-resolved fluorescent microspheres (10 mg / mL) and 900 μL of 0.01 mol / L boric acid-borax buffer solution with pH=7.4 into a 2 mL EP tube, Vortex for 20s, sonicate for 2min; centrifuge the mixture at 15000rpm, 4°C for 15min, discard the supernatant; use 1ml of 0.01mol / L boric acid-borax buffer with pH=7.4 to redissolve the precipitate, then add 20μl of 30g / L EDC solution for activation, the activation time is 15min. After the activated mixture was centrifuged at 15,000 rpm and 4°C for 15 min, the supernatant was discarded, and the precipitate was col...

Embodiment 2

[0060] The difference from Example 1 is that the concentration of the fluorescent microsphere-labeled mouse anti-human GDF-15 monoclonal antibody sprayed on the treated glass fiber is 0.2 mg / mL, and the concentration of the fluorescent microsphere-labeled rabbit IgG polyclonal antibody is 0.2 mg / mL. mg / mL, the rest are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Widthaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a test strip for quantitatively detecting GDF-15, and belongs to the technical field of immunobiology. The test strip for quantitatively detecting GDF-15 provided by the invention comprises a sample pad, a chromatography membrane and absorbent paper, a fluorescent microsphere labeled mouse anti-human GDF-15 monoclonal antibody and a fluorescent microsphere labeled rabbit IgG (immunoglobulin G) polyclonal antibody are sprayed on the sample pad; the chromatography membrane comprises a T detection line and a C quality control line, the T detection line is coated with a mouse anti-human GDF-15 polyclonal antibody, and the C quality control line is coated with a goat anti-rabbit IgG polyclonal antibody. The linear range of the test strip provided by the invention is 50-2000pg / mL, the lowest detection limit is 50pg / mL, the precision is not greater than 15%, the relative deviation between a test value and a theoretical value is within + / -15%, and the reagent can meet the performance parameters of the linear range, the lowest detection limit, the precision and the accuracy after being stored at 4-30 DEG C for 18 months.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to a test strip and a kit for quantitatively detecting GDF-15. Background technique [0002] Growth differentiation factor-15 (growth differentiation factor-15, GDF-15) is a distal branch of transforming growth factor β (transforming growth factor β, TGF-β) superfamily members, GDF-15 is a stress response protein, physiological Under normal circumstances, GDF-15 is highly expressed in the prostate and placenta, and weakly expressed in most other tissues, including heart tissue. However, under pathological and environmental stress conditions, such as ischemia / reperfusion injury, high pressure load on the heart, heart failure and atherosclerosis, GDF-15 is abundantly expressed in cardiomyocytes, and has a significant effect on cardiomyocyte structure and apoptosis program. Play a regulatory role, and the expression of GDF-15 also increases significantly under special physio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/558G01N33/577G01N33/58G01N33/543
CPCG01N33/6854G01N33/558G01N33/577G01N33/582G01N33/54306G01N2333/475
Inventor 孙长贵雷铮宇王德健曹志平陈汝彬陈珊珊赵彦孔佳能沈林
Owner HUNAN YONGHE YANGGUANG SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com