Biomimetic intestinal and liver microfluidic cell culture-drug screening integrated chip

Abstract

This application relates to the technical field of drug experiments. The application provides a bionic entero-liver microfluidic cell culture-drug screening integrated chip, which at least includes: a core plate, a bottom plate and a top plate; the core plate, Including the bionic intestinal cell area and the bionic hepatic cell area which are not connected to each other and placed on both sides of the chip; the bionic intestinal cell area includes the intestinal cell culture room and the vascular endothelial cell culture room; the intestinal cell culture room and the vascular endothelial cell culture room The extension direction of the chamber is on the same axis, the vascular endothelial cell culture chamber is located outside the intestinal cell culture chamber; a curved structure is set between the intestinal cell culture chamber and the vascular endothelial cell culture chamber; the bionic liver cell area includes: several There are two hepatocyte culture chambers arranged in a circumferential direction, and bionic capillary channels arranged between adjacent hepatocyte culture chambers. The scheme provided in this application can obtain more accurate data on the metabolism of drugs by organ cells.

Description

technical field [0001] The present application relates to the technical field of drug experiments, in particular, the present application relates to a bionic intestinal liver microfluidic cell culture-drug screening integrated chip. Background technique [0002] At present, one of the key issues in the fields of drug detection and toxicity screening, disease modeling, disease-related pathophysiology research, and intercellular interactions is how to construct biomimetic tissue structures in vitro. Taking drug screening as an example, it is to accurately simulate the subcellular structure and physiological response of cells in vivo, so as to accurately predict the toxic response of drugs in vivo. The development of new drugs usually includes preclinical drug safety evaluation, such as biochemical analysis, cytology experiments and animal toxicity experiments, and finally applied to the human body. However, most of the candidate new drugs are eliminated in phase III drug clin...

AI Technical Summary

Problems solved by technology

[0003] Moreover, although drug metabolism is completed in the liver, in reality, the absorption and metabolism of drugs is not completed by a single organ

Systemic absorption and metabolism of orally administered drugs mainly occurs in the small intestine and liver, however, in most in vitro studies, drug response studies in these two organs are still lacking for the toxicity of orally administered drugs

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