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Screening, preparation and application of a fermented barley protein with lipid-lowering activity

A barley protein and barley technology, which is applied to the preparation method of peptides, medical preparations containing active ingredients, fermentation, etc., can solve the problems of poor solubility, difficult absorption and utilization of barley protein, and achieve significant lipid-lowering functions and improve nutrition. Excellent performance and broad application prospects

Active Publication Date: 2022-08-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, barley protein has poor solubility and is difficult to be absorbed and utilized by the human body

Method used

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  • Screening, preparation and application of a fermented barley protein with lipid-lowering activity
  • Screening, preparation and application of a fermented barley protein with lipid-lowering activity
  • Screening, preparation and application of a fermented barley protein with lipid-lowering activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The present embodiment is to screen a fermented barley protein with lipid-lowering activity, which specifically includes the following steps:

[0044] S1: Preparation of fermented barley extract: pulverize the barley grains and pass through an 80-mesh sieve to obtain whole barley flour; degreasing the obtained barley flour with n-hexane, according to the mass-to-liquid ratio of 1:3, and magnetically stir at room temperature for 2 hours Then, suction filtration, repeat the above operation 2 to 3 times, and dry the obtained barley flour in a 37° C. oven to obtain defatted barley flour; the obtained defatted barley flour is subjected to liquid fermentation. According to the existing literature reports, fermented barley can improve the nutritional function of barley protein, but due to the variety of bacteria, and the fermentation effect and results of various fermenting bacteria are also affected by the amount added during fermentation, the ratio of material to liquid, and ...

Embodiment 2

[0056] The present embodiment is to prepare the fermented barley protein with lipid-lowering activity, which specifically includes the following steps:

[0057] 1) Determine the fermentation conditions: according to the screening method described in Example 1, the fermented barley protein with the best lipid-lowering ability is screened to determine the best fermentation conditions for its fermentation, extraction and purification preparation: the ratio of fermentation material to liquid is 1:7, The strain was Lactobacillus plantarum LP-1, the amount of Lactobacillus plantarum added per gram of barley flour was 7×107~9×107cfu, the initial pH value of fermentation was 5, the fermentation temperature was 31℃, and the time was 24h.

[0058] 2) Barley fermentation: carry out barley grain fermentation according to the determined optimal fermentation conditions for preparing lipid-lowering protein, and freeze-dry to obtain the total extract of fermented barley (specifically, the meth...

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Abstract

The invention discloses the screening, preparation and application of a fermented barley protein with lipid-lowering activity. First, the lipid-lowering activity is detected in combination with an oleic acid-induced HepG2 high-fat model, and fermented barley extracts obtained under different fermentation conditions are subjected to multi-stage separation Screening to obtain the fermented barley protein with the best lipid-lowering ability. Then, according to the screening results and the fermentation conditions and separation and purification conditions of the fermented barley protein with the best lipid-lowering ability, the fermented barley protein with lipid-lowering activity is prepared. The protein is used to prepare drugs or health food with lipid-lowering function, which further develops the nutritional function and medical value of barley, provides support for the further development and utilization of barley and the application of barley in the field of medical care, and has good economic and social value.

Description

technical field [0001] The invention relates to the screening, preparation and application of a fermented barley protein with lipid-lowering activity. Background technique [0002] Barley is one of the main crops grown in my country, and it is mainly used for beer brewing and as grass or roughage for livestock. Studies have found that barley is rich in functional proteins and polypeptides, covering most of human needs for amino acids, and is a valuable resource for developing healthy grain foods. [0003] However, barley protein has poor solubility and is difficult to be absorbed and utilized by the human body. Studies have found that the content of water-soluble protein in barley is increased through lactic acid bacteria fermentation, and the nutritional function of the fermented barley protein is improved. Studies have found that fermented barley protein can inhibit the synthesis of lipid droplets in adipocytes and reduce the differentiation rate of adipocytes. It can be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C07K14/415C07K1/36C07K1/30C07K1/18C07K1/16A23L33/185A61K38/16A61P3/06C12R1/25
CPCC07K14/415A23L33/185A61P3/06A23V2002/00A61K38/00A23V2200/3262A23V2250/5482Y02P60/87
Inventor 肖香张家艳赵延胜祝莹白娟邓欢
Owner JIANGSU UNIV