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Novel coronavirus antigen detection kit and preparation method thereof

A coronavirus and antigen detection technology, applied in the field of novel coronavirus antigen detection kits and their preparation, can solve the problem of slow detection speed, poor accuracy of results, and inability to meet the rapid screening of large-scale close contacts and asymptomatic infections, etc. problems, to achieve the effect of overcoming the long detection cycle

Pending Publication Date: 2022-03-01
江苏晶红生物医药科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The accurate detection of the new coronavirus in the current society still relies heavily on nucleic acid detection. This type of detection method is accurate, but the detection speed is slow, the cost is high, and a large number of professionally trained personnel are required to operate the test equipment, so large-scale rapid detection cannot be achieved. At present, most of the kits that can obtain test results at a faster speed rely on antibody detection. Although the cost is low and the operation is simple, the accuracy of the results is poor, and there is a high probability of false negatives and false positives. The situation, and can only be detected after 8-15 days of infection after the disease produces antibodies, which cannot meet the rapid screening of large-scale close contacts and asymptomatic infections

Method used

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  • Novel coronavirus antigen detection kit and preparation method thereof
  • Novel coronavirus antigen detection kit and preparation method thereof
  • Novel coronavirus antigen detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the preparation of colloidal gold solution

[0043] 1. Cleaning of glassware

[0044] The cleanliness of the glassware is directly related to the quality of the colloidal gold solution. If the utensils are not clean, the prepared colloidal gold solution will have uneven particle size, floating solids or turbidity. Immerse all the glassware required for the preparation of colloidal gold in 0.1mol / L sodium hydroxide solution for 2 hours, then rinse the glassware with purified water until neutral, then rinse the glassware with ultrapure water for three Put the rinsed utensils in an orderly manner into a blast drying oven, bake at 65°C for 8.5 hours, and put them in a clean finishing box for later use.

[0045] 2. Preparation of Colloidal Gold Solution

[0046] Colloidal gold solution was prepared by trisodium citrate reduction method; 100mL of ultrapure water was added to the conical flask, and 1.3mL of chloroauric acid solution with a mass concentration of...

Embodiment 2

[0049] Embodiment 2: Material preparation and assembly of colloidal gold kit

[0050] 1. Antibody labeling

[0051] Take 1mL of the above colloidal gold solution and place it in a 2mL EP tube, add 9μL of 0.2M potassium carbonate solution dropwise into the colloidal gold solution to adjust the pH of the solution, and then slowly and evenly add 15μg of mouse anti-SARS-Cov-2 to the solution NP monoclonal antibody 1, placed in the mixer at room temperature and mixed for 30 minutes; after mixing, quickly add 100 μL of 10% bovine serum albumin solution to the solution, placed in the mixer and continued to mix for 20 minutes, Take it out and let it stand at room temperature for 30 minutes; after standing, transfer the solution into a centrifuge and centrifuge at a speed of 13,000 rpm for 30 minutes; separate the precipitate after centrifugation, and dissolve the precipitate in 700 μL of colloidal gold resuspension buffer to obtain Resuspend the antibody in colloidal gold and store a...

Embodiment 3

[0061] Embodiment 3: Optimization of detection sample diluent

[0062] 1) Prepare different sample dilutions

[0063] Sample diluent a: configure a solution containing 0.05% Proclin300 and 0.9% sodium chloride;

[0064] Sample diluent b: prepare a solution containing 0.9% sodium chloride, 1% Tween 20 and 0.05% Proclin300;

[0065] Sample diluent c: prepare a solution of 0.01M PB containing 0.05% Proclin300 and pH7.4;

[0066] Sample diluent d: configure a solution containing 1% Tween 20, 0.05% Proclin300 and 0.01M PBS with pH7.4;

[0067] Sample diluent e: configure 0.01M PBS solution containing 0.5% Triton, 0.05% Proclin300 and pH7.4;

[0068] Sample diluent f: configure a solution containing 0.5% Triton, 0.3% EDTA and 0.01M PBS at pH 7.4;

[0069] Sample diluent g: prepare a solution containing 0.5% Triton, 0.05% SDS, 0.3% EDTA, 0.05% Proclin300 and 0.01M PBS with pH7.4.

[0070] 2) Use the sample diluent to detect and select the test card

[0071] a. First use the sam...

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Abstract

The invention discloses a novel coronavirus antigen detection kit and a preparation method thereof. The novel coronavirus antigen detection kit is composed of a card shell and a test strip located in the card shell, the test strip is composed of a bottom plate (1), a sample pad (2), a colloidal gold pad (3), a nitrocellulose membrane (4) and water absorption filter paper (5), and the sample pad (2), the colloidal gold pad (3), the nitrocellulose membrane (4) and the water absorption filter paper (5) are sequentially pasted on the bottom plate (1) from bottom to top. A test line T line (41) is arranged on the side, close to the colloidal gold pad (3), of the nitrocellulose membrane (4), and a quality control line C line (42) is arranged on the side, close to the water absorption filter paper (5), of the nitrocellulose membrane (4). The kit can directly detect whether a sample contains the novel coronavirus or not, and can be used for early diagnosis of diseases; nucleic acid detection is effectively assisted, and an effective scientific weapon is provided for the global combat for resisting epidemic situations.

Description

technical field [0001] The invention relates to the technical field of virus detection, in particular to a novel coronavirus antigen detection kit and a preparation method thereof. Background technique [0002] Up to now, the new type of coronavirus pneumonia is still raging and spreading around the world. Although my country has a good control of the epidemic situation, as the epidemic situation abroad is getting worse, the pressure on epidemic prevention in our country is also increasing. An important part of prevention and control has also attracted more and more attention. [0003] The accurate detection of the new coronavirus in the current society still relies heavily on nucleic acid detection. This type of detection method is accurate, but the detection speed is slow, the cost is high, and a large number of professionally trained personnel are required to operate the test equipment, so large-scale rapid detection cannot be achieved. At present, most of the kits that c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/558G01N33/548G01N33/58G01N33/532
CPCG01N33/56983G01N33/577G01N33/558G01N33/548G01N33/587G01N33/532G01N2333/165G01N2469/10
Inventor 张彦玉郑涛洪春花叶晗
Owner 江苏晶红生物医药科技股份有限公司
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