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A kind of pcr instrument with multi-temperature control module asynchronous optional channel and detection method thereof

A temperature control module and asynchronous technology, applied in the direction of chemical instruments and methods, biochemical equipment and methods, instruments, etc., can solve the problems of inexperienced and high cost, and achieve the effect of improving detection capabilities

Active Publication Date: 2022-04-22
SUZHOU MOLARRAY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the more channels configured, the higher the cost
From the perspective of reducing customer costs, the detection channel has an "optional" requirement; and how to realize the above-mentioned "optional" concept, there is currently no experience to refer to

Method used

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  • A kind of pcr instrument with multi-temperature control module asynchronous optional channel and detection method thereof
  • A kind of pcr instrument with multi-temperature control module asynchronous optional channel and detection method thereof
  • A kind of pcr instrument with multi-temperature control module asynchronous optional channel and detection method thereof

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Experimental program
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Embodiment 1

[0095]

[0096]

[0097] combined with Figure 1-Figure 4 As shown, a PCR instrument with multiple temperature control modules asynchronously optional channels, which includes: m thermal cover modules 100, m temperature control modules 200, m reaction pool assembly modules 300, main control board 400, fluorescence detection Component 500; wherein, m is a natural number greater than or equal to 2.

[0098] Wherein, the heat cover module 100 is used to seal the upper opening of the reaction cell assembly module 300 to increase the pressure and boiling point of the reagent; at the same time, it can also heat the reaction cell assembly module 300 .

[0099] Wherein, the temperature control module 200 is used for temperature control (heating and cooling) of the reaction pool assembly module 300; Heating is performed on the top of the reaction pool assembly module, so that the reagents in the reaction pool assembly module have a more uniform temperature field when the temperatu...

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Abstract

The invention discloses a PCR instrument with multiple temperature control modules asynchronously selectable channels and a detection method thereof; it belongs to the field of medical inspection and testing instruments and molecular diagnosis and detection instruments; its technical gist is that it includes: m temperature control modules, m A reaction pool component module and a fluorescence detection component; the fluorescence detection module is an excitation light-receiving light integrated module structure; the optical fiber fixing plate is arranged with through holes, the number of the through holes, optical fibers, and reaction pools is the same, and the optical fiber and fluorescence detection module They are respectively located on both sides of the optical fiber fixing plate; one end of the optical fiber is fixedly communicated with the through holes one by one, and the other end is inserted into the reaction pool. The present invention aims to provide a PCR instrument with multiple temperature control modules asynchronously optional channels and a detection method thereof. A set of fluorescence detection system can collect the fluorescence signals of m PCR reaction pools without interfering with each other; the channels are optional and can be To better meet the needs of users and reduce costs, the universal enhancement of equipment.

Description

technical field [0001] The present invention relates to the field of a medical examination instrument and a molecular diagnosis instrument, and more specifically relates to a PCR instrument with multiple temperature control modules asynchronously selectable channels and a detection method thereof. Background technique [0002] Fluorescence detection is a common detection method. [0003] Document 1: "Pang Lili, Bai Aiying, Qing Yu, et al. Establishment of immunofluorescence detection method for human H group rotavirus [J]. Acta Virology, 2020, 36(6):7", which reported the use of fluorescence detection for detection Rotavirus. [0004] Document 2: "Wu Haoxing, Liu Baihong, Liu Xuewei, et al. Establishment and performance evaluation of nano-fluorescent test strips for swine fever virus antibody [J]. Biotechnology Bulletin, 2020, 36(5):6", reported that fluorescence detection For the detection of swine fever virus. [0005] Document 3: "Bao Yaofei, Xue Qianru, Wu Haiping, Zo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/00G01N21/64
CPCB01L7/52G01N21/6428
Inventor 李晓刚陈振陈凯苏世维王野王威聂晶
Owner SUZHOU MOLARRAY CO LTD
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