Application of exogenous selenium-molybdenum composition in relieving chromium stress of tobacco
A technology of exogenous selenium and composition, applied in the field of agriculture, can solve the problems of cigarette product safety threats, harm to the health of smokers, and unclear mechanism, etc., to improve the antioxidant system, enhance the ability to resist heavy metal stress, and alleviate photosynthesis inhibitory effect
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[0033] Selection of raw materials: Select Yunyan 87, a tobacco variety widely planted across the country. Potassium dichromate is used as chromium, and the concentration used is 50 μmol / L.
[0034] Sources of reagents: Sodium selenite, sodium molybdate, and potassium dichromate solid powders were purchased from Zhengzhou Acme Chemical Co., Ltd., with a content of 99% analytically pure reagents.
[0035] Cultivation method: (1) pre-treatment of tobacco seeds: first sterilize with 10% sodium hypochlorite solution for ten minutes, then rinse repeatedly with ultra-pure deionized water, and soak the seeds for 8 hours; (2) point the treated tobacco seeds into the containing net In the deionized water seedling tray of grid sponge, 2-3 seeds are placed on each grid, and then placed in an incubator at 28°C for dark germination; (3) About three weeks after the seeds germinate, use tweezers to germinate The tobacco seedlings of 2 cotyledons were transplanted into commercially available ...
Embodiment 1
[0050] The effect of the application of exogenous selenium and molybdenum composition on the biomass of tobacco seedlings under chromium stress, see figure 1 . The plant height, root length, leaf dry weight and root dry weight of the above five groups of tobacco seedlings were measured respectively by using the above-mentioned method of measuring agronomic traits and biomass and measuring the fresh weight of each part of the tobacco sample.
[0051] Depend on figure 1 It can be seen that Cr stress significantly reduced the plant height, root length, leaf dry weight and root dry weight, while the application of Se, Mo and Se+Mo all alleviated the inhibitory effect of Cr on the growth of tobacco seedlings. Compared with CK, Cr treatment significantly reduced the plant height and root length by 16.8% and 42.6%, and the dry weight of leaves and roots of tobacco seedlings decreased by 28.0% and 38.9%, respectively. The addition of Se and Mo significantly promoted the growth of to...
Embodiment 2
[0054] The effect of the application of exogenous selenium and molybdenum composition on the content of chlorophyll in tobacco seedlings under Cr stress, see figure 2 . Using the above physiological and biochemical assay methods, the chlorophyll content of the above five groups of tobacco was measured respectively.
[0055] Photosynthesis and other physiological processes are highly sensitive to heavy metals, and low concentrations of Cr can also inhibit plant photosynthesis. Such as figure 2 It was shown that chromium stress significantly reduced the chlorophyll content of tobacco seedlings by 41.0%, 30%, and 38%, respectively. Studies have shown that the addition of selenium and molybdenum alone can increase the content of chlorophyll under chromium stress. The addition of Se and Mo can significantly increase the content of chlorophyll a by 26.7%, 36.2%, and the content of chlorophyll b by 34.7% and 101.1%. The content of T increased significantly by 38.1% and 33.9%, re...
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