Method for synthesizing L-fructose through whole-cell transformation

A whole-cell transformation and fructose technology, applied in the biological field, can solve the problems of difficult separation and purification of products, low conversion rate, etc., and achieve the effects of low price of raw materials, improved conversion rate, and reduced cost.

Pending Publication Date: 2022-03-22
DONGTAI HIRYE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the thermodynamic equilibrium involved, the conversion rate is low, and the product is difficult to separate and purify

Method used

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  • Method for synthesizing L-fructose through whole-cell transformation
  • Method for synthesizing L-fructose through whole-cell transformation
  • Method for synthesizing L-fructose through whole-cell transformation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method utilizing whole cells to transform and synthesize L-fructose, using glycerol as a substrate (glycerol concentration is 500mM), adding L-glyceraldehyde (addition amount is 40mM), pyrophosphate (addition amount is 80mM) and recombinant Escherichia coli After the induced wet bacteria (the final concentration of the wet bacteria reaction system is 100g / L), adjust the pH to 7.0, control the reaction temperature to 30°C, and catalyze the reaction for 12 hours (the above-mentioned entire reaction system is 10mL). After the end, the supernatant was collected by centrifugation for 10 minutes, and the solution containing L-fructose was obtained.

[0021] Wherein the recombinant Escherichia coli is a recombinant Escherichia coli simultaneously expressing acid phosphatase PhoN, phosphoglycerol oxidase GPO and L-rhamnosan-1-phosphate aldolase RhaD.

[0022] Preparation of wet cells:

[0023] 1) Construction of the recombinant plasmid pET28a-rhaD: Using the genome of Escher...

Embodiment 2

[0033] The difference from Example 1 is that the reaction temperature is 25°C.

Embodiment 3

[0035] The difference from Example 1 is that the reaction temperature is 45°C.

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Abstract

The invention relates to the technical field of biology, in particular to a method for synthesizing L-fructose by utilizing whole-cell transformation, a reaction system in the method comprises substrate glycerol, L-glyceraldehyde, pyrophosphoric acid and wet thalli of induced recombinant escherichia coli, and the L-fructose can be obtained after the reaction system is subjected to catalytic reaction. By utilizing the method, the yield of the L-fructose can be improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for synthesizing L-fructose by transforming whole cells. Background technique [0002] Rare ketohexoses are a class of monosaccharides and their derivatives that exist in nature but at very low levels and have important physiological functions. The L-configuration includes L-sorbose, L-fructose and L-tagatose, etc. It has the advantages of low calorie and natural sweetness, and can be used as a functional sweetener. It also has anti-cancer, anti-obesity, neuroprotection, Physiological activities such as scavenging free radicals have broad application prospects in the fields of life medicine, cosmetics, and diet. Therefore, it is of great significance to improve the rare sugar synthesis system. [0003] There are two methods for producing L-type rare ketohexose, chemical synthesis and biotransformation. The traditional chemical synthesis method has cumbersome steps, many s...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12P19/24C12N15/70C12N15/53C12N15/55C12N15/60
CPCC12P19/02C12P19/24C12N15/70C12N15/52C12N9/16C12N9/0006C12N9/88C12Y301/03002C12Y101/03021C12Y401/02019
Inventor 朱理平李子杰陈科才高晓冬陈洲胡扬帆
Owner DONGTAI HIRYE BIOTECH CO LTD
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