A nucleic acid detection kit and detection method

A detection kit and detection method technology, applied in the fields of biotechnology and medical detection, can solve the problems of low detection efficiency and complex nucleic acid detection process, achieve high detection efficiency, reduce the influence of nucleic acid double-stranded staining, ensure specificity and The effect of accuracy

Active Publication Date: 2022-05-03
BEIJING MICVIC BIOTECH CO LTD
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Problems solved by technology

[0004] In order to overcome the problems of complex nucleic acid detection process and low detection efficiency, the present invention provides a nucleic acid detection kit and detection method, which can accurately and quantitatively detect multiple nucleic acid target fragments in a relatively short period of time, fast, efficient, simple and safe

Method used

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  • A nucleic acid detection kit and detection method
  • A nucleic acid detection kit and detection method
  • A nucleic acid detection kit and detection method

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Embodiment 1

[0034] Example 1: To detect the HIV core fragment (residues 207 to 331), HCV core region gene fragment (C70-140), TP core fragment (Fla B1) and HBsAg core fragment (C protein) in the sample to be tested as an example.

[0035] 1. Materials and instruments

[0036] 1.1 Microfluidic chip

[0037] The composition of the chip card: including a sample loading area, a microchannel and a waste liquid area surrounded by a cover sheet and a substrate. The microchannel includes a detection area and a quality control area. The upper end of the sample hole is fixed with a nucleic acid dye SYBR Green I and a paired internal reference. Arabidopsis thaliana nucleic acid single strand, the detection area is set sequentially along the liquid flow direction T1 (coated with HIV core fragment nucleic acid single strand), T2 (coated with HCV core region gene fragment nucleic acid single strand), T3 (coated with TP Nucleic acid single strand of core fragment) and T4 (nucleic acid single strand co...

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Abstract

The invention proposes a nucleic acid detection kit and a detection method. The kit uses a microfluidic chip as a carrier, and includes a sample injection area, a microchannel and a waste liquid area. There are multiple T lines and C lines in the microchannel. The T line is coated with nucleic acid single strands containing different target fragments, and the C line is coated with internal reference single strands. After the nucleic acid in the sample to be tested is amplified and denatured into nucleic acid single strands, the nucleic acid single strands are mixed with nucleic acid dyes. Under ice bath conditions, it combines with the corresponding nucleic acid single strand coated on the T line to form a nucleic acid double strand stained with nucleic acid dye, thereby generating fluorescence and realizing the detection of multiple target fragments. This method is simple, convenient and efficient.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and medical detection, and in particular relates to a nucleic acid detection kit and a detection method. Background technique [0002] Nucleic acid detection has been widely used in many fields, including criminal investigation, detection of pathogenic microorganisms, and disease diagnosis. Under normal circumstances, the target nucleic acid content in environmental samples or physiological samples is very small. In order to make the results more accurate, a small amount of target fragments are often amplified, and then the amplified products are subjected to constant voltage electrophoresis. After the electrophoresis, the gel is taken out and placed Observe in the ultraviolet imager, take pictures and record the test results, and compare the size of the target fragment with the standard reference object, so as to obtain a negative or positive test result. This analysis method is mainly con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12Q1/6844
CPCC12Q1/6844C12Q2563/159
Inventor 李子熹
Owner BEIJING MICVIC BIOTECH CO LTD
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