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Liquid culture medium for culturing phellinus igniarius mycelium and culture method of phellinus igniarius mycelium

A technology of Phellinus linteus mycelium and liquid culture medium, applied in the field of microbial culture, can solve problems such as scarcity of Phellinus linteus, and achieve the effects of promoting rapid growth, short growth cycle, and maintaining vitality

Pending Publication Date: 2022-04-12
内蒙古科学技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies in the prior art, the present invention provides a liquid medium for cultivating Phellinus mycelium and its cultivation method. By adjusting the components and contents in the liquid medium and optimizing the cultivation method, the The rapid growth of the yellow mycelium provides the output of the Phellinus mycelium, which is conducive to solving the problem that the prior art is scarce to Phellinus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of Phellinus mycelia culture liquid culture medium, comprises the raw material of following parts by weight, in per liter:

[0027] Potato flour 70g, corn flour 50g, mulberry sawdust extract 15g, licorice extract 6g, glucose 4g, maltose 2g, yeast powder 8g, peptone 5g, potassium dihydrogen phosphate 2g, hydroxyapatite 3g, magnesium sulfate 1g, calcium carbonate 2g, vitamin B 0.02g, triammonium citrate 6g, magnesium acetate tetrahydrate 0.8g, and the balance is water.

[0028] Both the potato flour and corn flour are filtered through a 200-mesh sieve; the mulberry wood chip extract is prepared by the following preparation method. The mulberry wood chip is selected and crushed into 100 mesh mulberry wood chip powder, and ethanol with a mass concentration of 70% is added. The weight ratio of mulberry sawdust powder to ethanol was 1:10. After heating to 40°C, it was ultrasonically treated for 35 minutes, and the extraction was repeated 3 times. The extracts were comb...

Embodiment 2

[0036] A kind of Phellinus mycelia culture liquid culture medium, comprises the raw material of following parts by weight, in per liter:

[0037] Potato flour 60g, corn flour 40g, mulberry sawdust extract 10g, licorice extract 5g, glucose 6g, maltose 3g, yeast powder 8g, peptone 6g, potassium dihydrogen phosphate 4g, hydroxyapatite 3g, magnesium sulfate 2g, calcium carbonate 1g, vitamin B 0.05g, triammonium citrate 5g, magnesium acetate tetrahydrate 1.5g, and the balance is water.

[0038] Both the potato flour and corn flour are filtered through a 150-mesh sieve; the mulberry wood chip extract is prepared by the following preparation method. The mulberry wood chip is selected and ground into 80-mesh mulberry wood chip powder, and 60% ethanol is added. The weight ratio of mulberry sawdust powder to ethanol was 1:9. After heating to 50°C, it was ultrasonically treated for 30 minutes, and the extraction was repeated twice. The extracts were combined, concentrated and freeze-drie...

Embodiment 3

[0046] A kind of Phellinus mycelia culture liquid culture medium, comprises the raw material of following parts by weight, in per liter:

[0047] Potato flour 80g, corn flour 30g, mulberry sawdust extract 8g, licorice extract 6g, glucose 6g, maltose 4g, yeast powder 8g, peptone 10g, potassium dihydrogen phosphate 2g, hydroxyapatite 3g, magnesium sulfate 3g, calcium carbonate 2g, vitamin B 0.05g, triammonium citrate 4g, magnesium acetate tetrahydrate 1.2g, and the balance is water.

[0048] Both the potato flour and corn flour are filtered through a 150-mesh sieve; the mulberry sawdust extract is prepared by the following preparation method. The mulberry sawdust is selected and crushed into 80-mesh mulberry sawdust powder, and ethanol with a mass concentration of 65% is added. The weight ratio of mulberry sawdust powder to ethanol was 1:9. After heating to 40°C, ultrasonic treatment was performed for 35 minutes, and the extraction was repeated twice. The extracts were combined,...

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Abstract

According to the liquid culture medium for phellinus igniarius mycelium culture and the culture method of the phellinus igniarius mycelium culture, the corn flour and the potato powder are used as main nutrient substances, and the mulberry sawdust extract and the liquorice extract are added, so that rapid growth of phellinus igniarius mycelium can be promoted; the medicinal activity of the artificially cultured phellinus igniarius mycelium is equivalent to that of wild phellinus igniarius; meanwhile, functional components such as nano-scale hydroxyapatite, triammonium citrate and magnesium acetate tetrahydrate are added, so that the activity of phellinus igniarius strains is kept, and the growth of phellinus igniarius mycelia is promoted. Meanwhile, parameters such as the pH value, the culture temperature, the culture rotating speed and the amount of introduced air in the phellinus igniarius mycelium culture process are optimized, and rapid growth of the phellinus igniarius mycelium can be promoted. The phellinus igniarius mycelium culture method provided by the invention is simple to operate, has the advantages of short growth cycle and high fermentation mycelium yield, and can be used for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a liquid culture medium for Phellinus mycelia cultivation and a cultivation method thereof. Background technique [0002] Phellinus is a medicinal fungus belonging to the Basidiomycotina Phyllomycetes, also known as Phellinus baumannii, because its fruiting body has multiple functions such as anti-tumor and anti-oxidation, it has become a research hotspot at home and abroad. It is an internationally recognized fungus with the highest biological anti-cancer efficiency. [0003] In recent years, the increasing demand for Phellinus Phellinus in the market has made it impossible to eliminate the predatory exploitation of Phellinus Phellinus, and due to the particularity and complexity of its physiological ecology and the constraints of external conditions, the fruiting bodies formed in nature are very rare, wild and natural The resources of Phellinus Ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
Inventor 张秀娟侯亚光随洋
Owner 内蒙古科学技术研究院
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