Streptomyces toxytricini BFS-1 and application thereof

A technology of Streptomyces toxin and BFS-1, which is applied in the field of agricultural microorganisms to achieve the effect of small impact on the ecological environment and wide antibacterial spectrum

Active Publication Date: 2022-05-03
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of biocontrol bacteria that have a control effect on the stalk rot of Alocasia sagitta. Therefore, it is necessary to find new bacterial strains for the prevention and treatment of the stalk rot of Alocasia sagitta. more efficient options

Method used

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  • Streptomyces toxytricini BFS-1 and application thereof
  • Streptomyces toxytricini BFS-1 and application thereof
  • Streptomyces toxytricini BFS-1 and application thereof

Examples

Experimental program
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Embodiment 1

[0039] The isolation and identification of embodiment 1 bacterial strain

[0040] 1. Isolation and identification of strains

[0041] The strain was isolated from the rhizosphere soil of the shrub Scherichia spp. and named BFS-1. After cultivating the bacterial strain on Gao's No. 1 medium for 48 hours, it was observed that the single colony was round, pink, powdery on the surface, opaque, slightly raised, with white edges and radial bands, and the results were as follows: figure 1 shown.

[0042] 2. 16S rDNA molecular biology identification:

[0043] The genomic DNA of the strain was extracted as a template, and 16S rDNA bacterial universal primers (27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; 1429R: 5'-GGTTACCTT GTTACGACTT-3') were used for PCR amplification. Denaturation for 3 minutes, denaturation at 94°C for 30 seconds, annealing at 55°C for 1 minute, extension at 72°C for 90 seconds, 30 cycles, and finally extension at 72°C for 10 minutes; PCR system (25 μL): Taq PCR MasterMix 12...

Embodiment 2

[0046] The activity of embodiment 2 bacterial strain BFS-1 culture to Alocasia sagitta stem-base rot pathogen

[0047] The activity of living bacteria and strain culture of Streptomyces toxins BFS-1 strain was determined by plate confrontation method, and Streptomyces toxins BFS-1 strain was inoculated in Gaoshi No. 1 medium and cultured at 28°C for 48 hours. Use a puncher with an inner diameter of 6mm to punch holes on the Streptomyces toxin BFS-1 bacterial strain cultivated by Gao Shi No. 1, take a 6mm Streptomyces toxin BFS-1 bacterial block, and inoculate it at a distance of 2cm from the bacterial block F. elaeidis. Each treatment was repeated 3 times, and after 5 days of culture, the width of the inhibition zone was measured.

[0048] It has been determined that the live bacterial block of the BFS-1 strain has a significant inhibitory effect on F.elaeidis, and the results are as follows: figure 2 As shown, a bacteriostatic zone with an average bandwidth of 8.5mm was pr...

Embodiment 3

[0049] The antimicrobial spectrum of embodiment 3 bacterial strain BFS-1 cultures

[0050] In order to detect the antibacterial spectrum of Streptomyces toxins BFS-1 strain, the present embodiment adopts following several flower plant pathogens: Fusarium oxysporum (Fusarium oxysporum), Jasper blight (Phytophthora sp.) and Jianlan tiller rot (Erwinia sp. .) as the object, the antibacterial spectrum of the BFS-1 strain was determined by the zone of inhibition method.

[0051] Streptomyces toxini BFS-1 strain was inoculated on Gaoshi No. 1 solid plate medium, and cultured at 28°C for 48h. Use a hole puncher with an inner diameter of 0.6 mm to punch holes on the Gaoshi No. 1 solid plate culture, and take BFS-1 bacterial blocks. Inoculate the bacterial blocks of Fusarium wilt and Jasper blight on the medium at a distance of 3 cm from the BFS-1 bacterial block; add 1 mL of Jianlan tiller rot bacterial suspension to the melted PDA medium, pour it on the plate, and wait for it to sol...

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Abstract

The invention discloses streptomyces toxytricini BFS-1 and an application of the streptomyces toxytricini BFS-1. The streptomyces toxtricini BFS-1 strain is obtained by screening and separating from rhizosphere soil of shrub schefflera octophylla, the strain is preserved in Guangdong Microbial Culture Collection Center on September 11, 2020, and the preservation number of the strain is GDMCC No: 61183. The invention further discloses a preparation method of the streptomyces toxtricini BFS-1 strain. The research shows that the streptomyces toxytricini BFS-1 strain has a strong inhibition effect on the stem rot of alocasia macrorrhiza, the source is environment-friendly and non-toxic, and the influence on the ecological environment is small; besides, the streptomyces toxytricini BFS-1 strain has low requirements on culture conditions, has a relatively good inhibition effect on flower pathogenic bacteria fusarium, phytophthora and Erwinia pathogenic bacteria, is wide in antibacterial spectrum, and can be used for preventing and treating various plant diseases in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms. More specifically, it relates to a strain of Streptomyces toxin BFS-1 and its application. Background technique [0002] Fusarium is an important plant pathogenic fungus, which can infect various plants such as crops, vegetables, fruit trees and flowers, causing root rot and wilt, and seriously affecting production. Fusarium usually infects the rhizomes of plants, causing the roots to rot, the vascular bundles to turn brown, and the plants to wither and die. More famous diseases such as banana wilt, cucumber wilt and orchid wilt and so on. The disease caused by Fusarium usually has a slow onset process, and the affected plants die in about 15 to 30 days. Pathogenic bacteria often live through the winter in the soil as hyphae or chlamydospores, and spread through soil, running water, and agricultural operations. Once the disease occurs, it is difficult to prevent and control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P1/00C12R1/465
CPCC12N1/20A01N63/28Y02A50/30
Inventor 张云霞麦章龙林雁虹林接英聂丽婷谭清玲
Owner ZHONGKAI UNIV OF AGRI & ENG
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