Application of Prrc2b in preparation of medicine for treating demyelination disease

A technology of demyelination and myelination, applied in the field of biomedicine, which can solve the problems of few research results and so on

Active Publication Date: 2022-05-06
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the role of mRNA methylation modification in oligodendrocyte development has gradually become a research hotspot, but there are still relatively few research results on it. It is used

Method used

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  • Application of Prrc2b in preparation of medicine for treating demyelination disease
  • Application of Prrc2b in preparation of medicine for treating demyelination disease
  • Application of Prrc2b in preparation of medicine for treating demyelination disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 m 6 A binding protein detection

[0054] 1. Main experimental materials

[0055] C57BL / 6J mice, HA-prrc2b transgenic mice (provided by Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences), Protein A / G magnetic beads (Bimake), Pierce TM Anti-HA-tag magnetic beads (Thermo Scientific), TRIzol (Invitrogen), BCA kit (Beiyuntian Biotechnology), RNasin (Promega), proteinase K (Roche), MNase (Biolabs).

[0056] 2. Experimental method

[0057] 1) In vitro RNA pull-down

[0058] Brain tissue of embryonic E16 mice (C57BL / 6J mice) was isolated, protein was extracted and BCA was quantified. Protein lysate pretreatment: incubate magnetic beads with lysate at 4°C for 1 h to remove non-specific binding; streptomycin-linked magnetic (ThermoScientific) pretreatment: mix magnetic beads with 0.2 mg / ml tRNA (Sigma) and 0.2 mg / ml BSA was incubated at 4°C for 1 h to block the non-specific sites on the magnetic beads. The pretreated protein lysate and...

Embodiment 2

[0070] Example 2 Physiological function research of Prrc2b

[0071] 1. Experimental materials

[0072] C57BL / 6J mice, Prrc2b conditional knockout mice, MBP antibody (Abcam), Olig2 antibody (Millipore), Pdgfrα antibody (BD Bioscience), Trizol (Invitrogen), BCA kit (Beyond Biotech), Black-GoldⅡ Myelin staining kit (Millipore), CC1 antibody (Millipore).

[0073] 2. Experimental method

[0074] 1) Breeding Prrc2b conditional knockout mice

[0075] Prrc2b f / f The mouse (constructed by Dr. Wang Fengchao, Beijing Institute of Life Sciences, donated by Dr. Zhang Jinhua, Beijing Jiaotong University) and the Olig2-Cre mouse (gifted by Prof. Xiao Bo, Sichuan University, Stock No. 011103, JacksonLaboratory) were crossed to obtain Prrc2b f / + ; Olig2-Cre mice. Prrc2b f / f Mice and Prrc2b f / +; Olig2-Cre mice were crossed to obtain offspring Prrc2b f / f , Prrc2b f / + ; Olig2-Cre and Prrc2b knockout of the Prrc2b gene in the Olig2-expressing oligodendrocyte lineage f / f ; Olig2-Cre.

[...

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PUM

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Abstract

The invention discloses an application of Prrc2b in preparation of a medicine for treating a demyelination disease. Experiments prove that Prrc2b is a novel m6A binding protein and regulates and controls the oligodendrocyte fate decision, and Prrc2b gene knockout can cause insufficient myelination or demyelination, so that Prrc2b can be used as a drug target for treating the demyelination disease. In addition, the invention also discloses an application of Prrc2b in construction of a myelin injury or demyelination animal model.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of Prrc2b in the preparation of medicines for treating demyelinating diseases. Background technique [0002] Myelin is a layer of myelin protein wrapped around the nerve fibers, which can wrap the axons to insulate the axons from each other and avoid interference; the Ranvier knot formed between the myelin sheaths can make the action potential "jump" on the axons Conduction" to speed up signal transmission; in addition, myelin wrapping around axons can also play a protective role. [0003] Can help axon regeneration in case of axon injury. When the myelin sheath is damaged or underdeveloped, it will cause some myelin sheath-related diseases, such as multiple sclerosis, optic neuromyelitis, etc., and studies have found that a variety of neurological diseases are accompanied by myelin sheath loss, such as Alzheimer's disease, depression syndrome and brain trauma. The cell...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K45/00A61P25/00G01N33/53C12Q1/6869
CPCA01K67/0276G01N33/5308C12Q1/6869A61K45/00A61P25/00A01K2217/15A01K2227/105A01K2267/03C12Q2535/122C12Q2537/164
Inventor 袁增强王树坤张莹李硕硕孔祥熙吴荣
Owner ACADEMY OF MILITARY MEDICAL SCI
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