Rolling type cell culture instrument

A cell culture and rolling technology, applied in tissue cell/virus culture devices, biochemical instruments, chemical instruments and methods, etc., can solve the problems of time-consuming and laborious, inability to add reagents, increase the area of ​​the inner wall, etc., and achieve distribution Uniformity, convenient cell separation, and the effect of improving work efficiency

Pending Publication Date: 2022-05-10
蓝莲(杭州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the roller bottles on the market only increase the area of ​​the inner wall, and cannot realize automatic reagent addition, cell separation, bottle cleaning and other operations, which are time-consuming and labor-intensive. The market needs a rolling cell culture instrument that can realize automatic production.

Method used

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  • Rolling type cell culture instrument
  • Rolling type cell culture instrument

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Embodiment Construction

[0025] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] The rolling cell culture apparatus includes: a cell culture flask 1, a rotating shaft assembly that runs through the cell culture flask 1 and drives the cell culture flask 1 to rotate, a reagent delivery tube 4 connected to the cell culture flask 1, and connected to the reagent delivery tube 4 The multi-directional water pump 5 is connected to the connecting pipe 6 of the multi-directional water pump 5, the reagent pipe 7 arranged on the connecting pipe 6, the connecting valve 8 arranged between the reagent pipe 7 and the connecting pipe 6, is connected to the multi-directional water pump 5 The collection pipeline 9. As a preference, the multi-directional water pump 5 is a three-way water pump; the connecting valve 8 is an electronic flow valve.

[0027] The rotating shaft assembly includes: a rotating shaft 2 , a rotating motor driving...

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PUM

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Abstract

The invention discloses a rolling type cell culture instrument which comprises a cell culture bottle, a rotating shaft assembly which is arranged on the cell culture bottle in a penetrating mode and drives the cell culture bottle to rotate, a reagent conveying pipe connected to the cell culture bottle, a multidirectional water pump connected to the reagent conveying pipe, a connecting pipe connected to the multidirectional water pump and a reagent pipe arranged on the connecting pipe. The connecting valve is arranged between the reagent pipe and the connecting pipe; the collecting pipeline is connected to the multidirectional water pump; the device can realize a series of operations such as automatic liquid adding, cleaning, disinfection and cell separation, so that the working efficiency is improved, and the labor is reduced.

Description

technical field [0001] The invention relates to the field of cell culture equipment, in particular to a rolling cell culture apparatus. Background technique [0002] Animal cell culture is the removal of the relevant tissue from the animal body, dispersing it into individual cells (using trypsin or collagenase) and then placing them in a suitable medium to allow these cells to grow and proliferate. [0003] Animal cells are generally attached to culture flasks, so in actual production, cylindrical cell culture roller bottles used to expand adherent or suspension growing cells have many advantages. The advantages of using roller bottle culture are low cost, mature technology and easy operation. In production, the number of roller bottles can be increased as needed to increase the amount of cell culture. [0004] However, the roller bottles on the market only increase the area of ​​the inner wall, and cannot realize automated addition of reagents, cell separation, bottle cle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/02C12M3/04C12M1/24C12M1/10C12M1/12C12M1/02C12M1/38C12M1/36C12M1/04B08B3/02
CPCC12M23/08C12M23/06C12M23/38C12M27/12C12M33/14C12M29/06C12M29/00C12M33/04C12M37/00C12M39/00C12M41/18C12M41/48C12M41/44B08B3/02
Inventor 杨罕闻
Owner 蓝莲(杭州)生物科技有限公司
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