Primer and probe combination and kit for methylation detection of cervical cancer and use method of kit

Abstract

The invention discloses a primer and probe combination and a kit for methylation detection of cervical cancer and a use method of the kit. The primer and the probe are used for detecting gene combinations of PAX1, EPB41L3 and FAM19A4; the primer and probe combination comprises a PAX1 forward primer F, a PAX1 reverse primer R, a PAX1 probe P, an EPB41L3 forward primer F, an EPB41L3 reverse primer R, an EPB41L3 probe P, an FAM19A4 forward primer F, an FAM19A4 reverse primer R and an FAM19A4 probe P; the nucleotide sequences of the primers are respectively shown as SEQ ID NO: 1-SEQ ID NO: 9. The probe adopted by the invention has the advantages of high specificity and high sensitivity, and can accurately and stably detect 5% methylation rate as low as 1ng / mu L; and the probe has a single base resolution ratio, and can specifically distinguish methylated DNA from unmethylated DNA.

Description

technical field [0001] The invention relates to the technical field of tumor gene detection, in particular to a combination of primers and probes, a kit and a use method for detecting cervical cancer methylation. Background technique [0002] Cervical cancer is one of the common gynecological malignancies. The peak age of the disease is 40 to 60 years old. In recent years, the age of onset of cervical cancer has shown a younger trend. Studies have shown that the induction of cervical cancer is related to the pathogen of human papillomavirus (HPV). After high-risk HPV infection, the occurrence of cervical cancer needs to go through multiple steps: from persistent high-risk HPV infection, to precancerous lesions (CIN1→CIN2→CIN3), and finally to invasive cancer. The occurrence and development of cervical cancer, especially precancerous lesions to invasive cancer, most patients need to go through a long period of time. High-risk HPV infection can progress to CIN2 and CIN3 stag...

AI Technical Summary

Problems solved by technology

The specificity of cytological examination is high, but the sensitivity is low, and the judgment of cytological results requires professional pathologists to interpret under the microscope. The results are subject to subjective influence, and it is easy to miss diagnosis and misdiagnosis. Moreover, due to the shortage of pathologists, low Resource areas are difficult to popularize

The sensitivity of HPV detection is high, but the specificity is poor, and its false positive rate is relatively high. Most HPVs are transient infections and will not develop into cervical precancerous lesions or cervical cancer. Positive results of HPV have caused unnecessary harm to women. Panic and waste of medical resources

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