Method for accurately detecting liquid-based fungi by using pelleter

A technology for detecting liquid and tableting machine, which is applied in the field of medical testing, can solve the problems of cumbersome operation, long process time, many reagents, etc., and achieves the effect of solving cumbersome operation, simple preparation method and good fluorescence effect.

Inactive Publication Date: 2022-05-10
HUBEI KANGQIANG MEDICAL DEVICES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for accurately detecting liquid-based fungi using a tablet machine. The purpose is that the preparation method is simple, and the prepared fungal flakes are clear and observable, so as to realize accurate detection of fungi and effectively solve the problems in the prior art. Existing problems such as cumbersome operation, long process, and many reagents required

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for accurately detecting liquid-based fungi using a tablet machine, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal specimen, the volume ratio of the sample and the special digestive solution is 1:1, and turning the bottom of the bottle into an inverted shape. Put the cone-shaped sample bottle into the shaker and vibrate for 5 minutes, let it stand for 10 minutes, with the tip facing down, fungi with a large specific gravity will settle at the bottom of the tip, shake for 5 minutes, insert a disposable pipette into the bottom of the sample bottle, suck 1mL of the sample, add to In the film production chamber, the film production chamber is installed on the film production frame equipped with glass slides, and the film production frame is suspended into the centrifugal liquid-based cell film maker, and centrifuged at 800r / min for 3min to make liquid-based thin slices. After the liquid-based thin section...

Embodiment 2

[0030] A method for accurately detecting liquid-based fungi using a tablet machine, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal specimen, the volume ratio of the sample and the special digestive solution is 1:1, and turning the bottom of the bottle into an inverted shape. Put the cone-shaped sample bottle into the shaker and vibrate for 10 minutes, let it stand for 30 minutes, with the tip facing down, fungi with a larger specific gravity will settle at the bottom of the tip, shake for 10 minutes, insert a disposable pipette into the bottom of the sample bottle, suck 2mL of the sample, add to In the film production chamber, the film production chamber is installed on the film production rack equipped with glass slides, and the film production rack is suspended into the centrifugal liquid-based cell film maker, and centrifuged at 1000r / min for 5min to make liquid-based thin slices; After the liquid-based thin secti...

Embodiment 3

[0036] A method for accurately detecting liquid-based fungi using a tablet machine, comprising the following steps: adding a special digestive solution into a sample bottle containing a fungal specimen, the volume ratio of the sample and the special digestive solution is 1:1, and turning the bottom of the bottle into an inverted shape. Put the cone-shaped sample bottle into the shaker and vibrate for 6 minutes, let it stand for 15 minutes, with the tip facing down, fungi with a larger specific gravity will settle at the bottom of the tip, shake for 6 minutes, insert a disposable pipette into the bottom of the sample bottle, suck 1mL of the sample, add to In the film production chamber, the film production chamber is installed on the film production rack equipped with glass slides, and the film production rack is suspended into the centrifugal liquid-based cell film maker, and centrifuged at 850r / min for 4min to make liquid-based thin slices; After the liquid-based thin section ...

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PUM

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Abstract

According to the method for accurately detecting the liquid-based fungi by using the pelleter, the special coloring agent is added with phycocyanobilin or phycoerythrin with excellent fluorescence, is red, purple blue and blue protein with strong fluorescence, is derived from algae substances such as blue-green algae or red algae, is natural and non-toxic, and can be used for accurately detecting the liquid-based fungi. Compared with a common staining agent, the fluorescent staining agent is better in fluorescent effect, safer, free of carcinogenesis and suitable for staining for fungus detection; based on a liquid-based flaking technology of a centrifugal method, a centrifugal oscillation method is adopted, fungi are separated into single fungal cells without adhesion, most of human cells and other bacteria in leucorrhea are subjected to enzymolysis after digestion, only the fungal cells are left on a slice after thin-layer filtration, and the cells are in a suspension state after oscillation, so that the liquid-based flaking is realized. Cells dyed by the dyeing agent are clear and distinguishable, and microscopic observation is facilitated.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a method for accurately detecting liquid-based fungi using a tablet machine. Background technique [0002] Fungi belong to eukaryotes, without plastids, and their nutritional mode is absorption without phagocytosis. The cell wall contains chitin and β-glucan. It can cause human and animal diseases, called fungal diseases, and can also cause plant diseases, human allergic diseases and mycotoxin poisoning. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors and diabetes; large doses of X-ray irradiation; immunosuppressants, etc. lead to low immune function and resistance; The reaction is mostly suppressed, and it is also easy to be infected with fungi; in addition, organ transplantation, catheter intubation, radiotherapy and other applications cause local tissue damage, which provides conditions...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N21/64
CPCG01N1/2813G01N1/30G01N21/6458G01N2001/302
Inventor 刘国华
Owner HUBEI KANGQIANG MEDICAL DEVICES CO LTD
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