Method for reducing blood sugar and blood fat of hyperlipemia rats
A technology for hyperlipidemia and blood lipids, applied in the field of hyperlipidemia, can solve the problems of liver and kidney damage, elevated blood lipids, high morbidity, improve the degree of degeneration, control blood sugar well, reduce liver weight and epididymal circumference The effect of fat weight
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[0025] A method for reducing blood sugar and blood fat in hyperlipidemic rats, comprising the following steps:
[0026] S1. Prepare experimental animals, experimental materials and experimental instruments for backup;
[0027] S2. Take 5g of potato resistant starch, add hydrochloric acid-potassium chloride buffer, add 1mL of pepsin solution, shake at 40°C for 1h, adjust pH=6.0, add 1mL of high temperature resistant α-amylase solution, and then add glucose Amylase solution 1mL, shake at 70°C for 1h, hydrolyze digestible starch into glucose, cool at room temperature, centrifuge, discard supernatant, 3 times, add 5mol / L potassium hydroxide solution 5mL, add glucoamylase solution 1mL, Shake at 60°C for 1 hour, cool at room temperature, centrifuge, combine supernatants, and dilute to 100mL;
[0028] S3. Determination of reducing sugar content in potato resistant starch by sulfuric acid-phenol method, the calculation formula for the yield of potato resistant starch is as follows: ...
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