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Method for reducing blood sugar and blood fat of hyperlipemia rats

A technology for hyperlipidemia and blood lipids, applied in the field of hyperlipidemia, can solve the problems of liver and kidney damage, elevated blood lipids, high morbidity, improve the degree of degeneration, control blood sugar well, reduce liver weight and epididymal circumference The effect of fat weight

Pending Publication Date: 2022-05-17
内蒙古自治区综合疾病预防控制中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] A high-fat diet can lead to elevated blood lipids. In severe cases, it can cause a series of glucose and lipid metabolism diseases, such as hyperlipidemia. Long-term consumption of a high-fat diet can cause abnormal accumulation of body fat and imbalance of intestinal microbiota. Hyperlipidemia is Metabolic diseases caused by lipid deposition, characterized by elevated blood cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) Its main feature is the reduction of blood pressure, high incidence and many complications. In recent years, the number of patients with hyperlipidemia has gradually increased. Hyperlipidemia has become an important risk factor for cardiovascular and cerebrovascular diseases. It is a chemical drug, although its curative effect is remarkable, but it has certain damage to the liver and kidney

Method used

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  • Method for reducing blood sugar and blood fat of hyperlipemia rats
  • Method for reducing blood sugar and blood fat of hyperlipemia rats
  • Method for reducing blood sugar and blood fat of hyperlipemia rats

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Embodiment 1

[0025] A method for reducing blood sugar and blood fat in hyperlipidemic rats, comprising the following steps:

[0026] S1. Prepare experimental animals, experimental materials and experimental instruments for backup;

[0027] S2. Take 5g of potato resistant starch, add hydrochloric acid-potassium chloride buffer, add 1mL of pepsin solution, shake at 40°C for 1h, adjust pH=6.0, add 1mL of high temperature resistant α-amylase solution, and then add glucose Amylase solution 1mL, shake at 70°C for 1h, hydrolyze digestible starch into glucose, cool at room temperature, centrifuge, discard supernatant, 3 times, add 5mol / L potassium hydroxide solution 5mL, add glucoamylase solution 1mL, Shake at 60°C for 1 hour, cool at room temperature, centrifuge, combine supernatants, and dilute to 100mL;

[0028] S3. Determination of reducing sugar content in potato resistant starch by sulfuric acid-phenol method, the calculation formula for the yield of potato resistant starch is as follows: ...

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Abstract

The invention relates to the technical field of hyperlipemia, and discloses a method for reducing blood sugar and blood fat of a hyperlipemia rat, at present, medicines commonly used clinically are chemical medicines and have obvious curative effects but have certain damage to the liver and kidney, S1, experimental animals, experimental materials and experimental instruments are prepared for standby application, S2, 5g of potato resistant starch is taken, and S3, the potato resistant starch is added into the experimental animals, the experimental materials and the experimental instruments are added into the experimental animals, the experimental materials and the experimental instruments for standby application. Adding a hydrochloric acid-potassium chloride buffer solution, then adding 1mL of a pepsin solution, oscillating at 40 DEG C for 1 hour, adjusting the pH value to 6.0, adding 1mL of a high-temperature-resistant alpha-amylase solution, then adding 1mL of a glucamylase solution, oscillating at 70 DEG C for 1 hour, hydrolyzing digestible starch into glucose, cooling at room temperature, centrifuging, and discarding supernate. The potato resistant starch has good effects of controlling blood sugar, reducing serum TC, TG, LDL-C and AI levels of hyperlipemia rats, and reducing liver weight and epididymal fat weight, not only can effectively improve the degree of fat degeneration, but also can effectively prevent and treat hyperlipemia.

Description

technical field [0001] The invention relates to the technical field of hyperlipidemia, in particular to a method for reducing blood sugar and blood fat in rats with hyperlipidemia. Background technique [0002] A high-fat diet can lead to elevated blood lipids. In severe cases, it can cause a series of glucose and lipid metabolism diseases, such as hyperlipidemia. Long-term consumption of a high-fat diet can cause abnormal accumulation of body fat and imbalance of intestinal microbiota. Hyperlipidemia is Metabolic diseases caused by lipid deposition, characterized by elevated blood cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) Its main feature is the reduction of blood pressure, high incidence and many complications. In recent years, the number of patients with hyperlipidemia has gradually increased. Hyperlipidemia has become an important risk factor for cardiovascular and cerebrovascular di...

Claims

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Application Information

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IPC IPC(8): A61K31/718A61K36/81A61P3/10A61P3/06A61K49/00A01K67/02
CPCA61K31/718A61K36/81A61P3/10A61P3/06A61K49/0008A01K67/02
Inventor 吴建美丁玉林曲琳尹幸念樊树理尉迟邈
Owner 内蒙古自治区综合疾病预防控制中心
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