Culture medium and method for inducing one-step seedling formation of cremastra appendiculata pseudobulb plants

A culture medium formula and culture medium technology, applied in botany equipment and methods, horticultural methods, applications, etc., can solve problems such as unstable inheritance of good traits, wildly different biological yields per plant, and restrictions on large-scale production. Achieve the effects of improving domestication survival rate, simplifying production steps, increasing differentiation and rooting rate

Active Publication Date: 2022-06-24
SOUTHWEST FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, because Rhododendron orchid has many origins and varieties, the medicinal efficacy and biological yield of a single plant vary widely among different origins and varieties; at the same time, the seeds are all obtained through hybridization, and genetic instability may occur when high-quality varieties are selected for propagation. On the other hand, the existing tissue culture technology needs to germinate seeds into protocorms, protocorms proliferate and differentiate into seedlings with buds, leaves and other organs, and then the seedlings take root. The process is relatively complicated; moreover, under the existing technical conditions, the differentiation rate is low, which greatly limits the large-scale production during tissue culture and increases the production cost

Method used

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  • Culture medium and method for inducing one-step seedling formation of cremastra appendiculata pseudobulb plants
  • Culture medium and method for inducing one-step seedling formation of cremastra appendiculata pseudobulb plants
  • Culture medium and method for inducing one-step seedling formation of cremastra appendiculata pseudobulb plants

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:

[0028] 1. Explant selection and cleaning: Choose Rhododendron orchid, transplant it indoors, irrigate the roots with 75% (v / v) 500 times of carbendazim every 3 days, and irrigate the roots continuously for 4 times; After the root is completed, take the underground pseudobulb, remove the stems and leaves on the ground and the root system on the surface, rinse with saturated soapy water solution for 10 minutes, rinse with tap water 3 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 2 drops of Tween- 80, shake for 15 minutes, then rinse with tap water for 60 minutes;

[0029] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 20s, rinse with sterile water for 2 times, and disinfect with saturated bleach solution for 18min, steri...

Embodiment 2

[0034] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:

[0035] 1. Explant selection and cleaning: Choose Rhododendron orchid, transplant it indoors, irrigate the roots with 75% (v / v) 500 times of carbendazim every 3 days, and irrigate the roots continuously for 5 times; After the root is completed, take the underground pseudobulb, remove the stems and leaves on the ground and the root system on the surface, rinse it with saturated soapy water solution for 12 minutes, rinse it with tap water 4 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 3 drops of Tween- 80, shake for 13 minutes, then rinse with tap water for 70 minutes;

[0036] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 25s, rinse with sterile water for 3 times, disinfect with saturated bleaching powder solution for ...

Embodiment 3

[0042] A method for utilizing Rhododendron orchid pseudobulbs to induce plants to grow into seedlings in one step, the steps comprising:

[0043] 1. Selection and cleaning of explants: Choose Rhododendron orchid, transplant them indoors, irrigate the roots with 500 times of 75% (v / v) carbendazim every 3 days, and irrigate the roots continuously for 5 times; After irrigating the roots, take the underground pseudobulbs, remove the stems, leaves and surface roots, rinse with saturated soapy water solution for 15 minutes, rinse with tap water 4 times, scrape off the raised and depressed parts of the epidermis with a knife, and then add 3 drops of Tween -80, shake for 15 minutes, and then rinse with tap water for 80 minutes.

[0044] 2. Disinfection of explants: transfer the washed explants to an ultra-clean workbench, disinfect with 75% (v / v) alcohol for 25s, rinse with sterile water for 3 times, disinfect with saturated bleaching powder solution for 20min, sterile Washing with w...

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Abstract

The invention discloses a method for inducing plants to form seedlings in one step by utilizing cremastra appendiculata pseudobulb and application. The method comprises the following steps: selecting and cleaning explants: selecting underground pseudobulb, rinsing with saturated soapy water and tap water, scraping skin bulges and pits, adding Tween-80, shaking, and showering with tap water; disinfection of explants: disinfecting the explants with alcohol, saturated bleaching powder, bromogeramine and a mercuric chloride solution in sequence; protocorm induction culture: cutting the protocorm into small blocks with epidermis, and inoculating the small blocks into a protocorm induction culture medium; and one-step seedling culture: cutting the protocorm into small blocks, and inoculating the small blocks into a one-step seedling culture medium for culture. The protocorm is subjected to one-step seedling culture, complete plants can be directly obtained, commercial seedlings are formed, and the plant differentiation rate is 100%; during differentiation, rooting and pseudobulb formation are realized, the rooting rate can reach 98%, and the pseudobulb formation rate can reach 92%; the differentiation rate and the rooting rate are high, the production cost is greatly saved, the pseudobulb formation rate is high, and the domestication survival rate is greatly increased.

Description

technical field [0001] The invention relates to the field of tissue culture of medicinal plants and Chinese medicinal materials, in particular to a method and application for inducing plants to grow into seedlings in one step by using pseudobulbs of Rhododendron orchid. Background technique [0002] The dried pseudobulb of Rhododendron orchid is used as medicine, and its medicinal material is called Mushroom, which is one of the source plants of Mushroom in the Chinese Pharmacopoeia. Shanci Mushroom has the functions of clearing away heat and detoxifying, resolving phlegm and dispersing knots. It is mainly used for the treatment of carbuncle and furuncle poisoning, scrofula and phlegm nucleus, snake and insect bites, lameness and other diseases. Modern medical research shows that the mushroom contains 7-hydroxy-2,4-dimethoxyphenanthrene and other phenanthrene compounds, 3,5,3'-trihydroxybibenzyl and other bibenzyl compounds, cinnamic acid, Aromatic compounds such as tea aci...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 向建英曾祥飞邓国宾张媛王德新张文娟
Owner SOUTHWEST FORESTRY UNIVERSITY
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