Compound bacterium preparation for sow midwifery and preparation method and application thereof
A technology of complex bacteria and preparations, applied in the fields of application, food processing, animal husbandry, etc., can solve the problems of difficult balance of pig micro-ecosystem, susceptibility to pathogenic bacteria, increased mortality of newborn piglets, etc., and achieve improved prevention and control of pig-related problems Diseases, prevention of germ invasion, and promotion of piglet vitality recovery
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[0059] The present invention also provides the preparation method of the compound bacterial preparation described in the above technical scheme, comprising the following steps:
[0060] first mixing probiotics, prebiotics and yucca extract to obtain a first mixture;
[0061] The first mixture, the montmorillonite and the flavoring agent are mixed for a second time to obtain the composite bacterial preparation.
[0062] Before performing the first mixing, the present invention preferably further comprises separately pulverizing probiotics, prebiotics and yucca extracts to obtain probiotics powder, prebiotics powder and yucca extract powder, respectively. The particle size of the probiotic powder, prebiotic powder and yucca extract powder of the present invention is preferably 200 meshes. In the present invention, an ultrafine pulverizer is preferably used for the pulverization, and the source and model of the ultrafine pulverizer are not particularly limited in the present inv...
Embodiment 1
[0082] Screening conditions for Bacillus subtilis, Bacillus licheniformis, Bacillus natto, and Bacillus pumilus (Bacillus subtilis, Bacillus licheniformis, Bacillus natto, and Bacillus pumilus are all screened according to this method, and will not be repeated):
[0083] Weigh 5g of fresh pig manure and environmental samples, mix the pig manure and environmental samples into a 100mL triangular flask containing sterile saline, shake them evenly, and place them in a constant temperature water bath at 80°C for 15 minutes. minutes, a certain amount of the sample supernatant was added to 100 mL of LB broth medium, and incubated at 37° C., 200 r / min on a shaker for 24 hours.
[0084] Take 1mL of the above shaker culture medium and dilute it to 10 times with sterile normal saline. -1 , 10 -3 , 10 -5 100 μL of each diluted bacterial solution was taken and spread on LB solid medium respectively, and placed in an incubator at 37° C. for 24-36 hours. A single colony on the LB solid me...
Embodiment 2
[0105] A compound bacterial preparation for sow delivery is made up of the following components, and the following components are the bacterial powder of the corresponding bacterial species prepared in Example 1:
[0106] 1 part of Bacillus subtilis powder, 0.5 part of Bacillus natto powder, 0.5 part of Bifidobacterium animalis powder, 0.5 part of soybean oligosaccharide, 0.25 part of isomalto-oligosaccharide, 0.25 part of xylo-oligosaccharide, Yucca extract 0.1 part of material, 800 parts of sodium montmorillonite and 1 part of fragrance.
[0107] Specifically, Bacillus subtilis powder 125g, Bacillus natto powder 62.5g, Bifidobacterium animalis powder 62.5g, soybean oligosaccharide 62.5g, isomalt oligosaccharide 31.25g, xylo-oligosaccharide 31.25g, yucca Extract 12.5g, sodium montmorillonite 100kg and fragrance 125g.
[0108] The components in the same weight unit ratio are used in the following examples, and will not be repeated.
[0109] Preparation method: in an environm...
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