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Biological enzyme prepared from pigs died of diseases and preparation method thereof

A technology of biological enzymes and dead animals, applied in microbial treatment, combined biological treatment, preparation of organic fertilizers, etc., can solve the problems of easy generation of foul gas and sewage, short time consumption, high cost, and achieve rich varieties, product safety, To achieve the effect of recycling

Pending Publication Date: 2021-12-28
SICHUAN JIENENG DRYING EQUIP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can completely eliminate pathogenic bacteria and takes a short time, but a large amount of unpleasant gas will be generated during the incineration process, which consumes a lot of energy, and it is not possible to recycle sick and dead pigs
3. The chemical method is to use steam pressure or dry heat pressure to treat sick and dead pig carcasses in a closed container. The specific treatment time is short, the treatment capacity is large, and the treatment is simple; but the cost is high, and it is easy to produce foul-smelling gas and sewage
This method has low cost, simple operation, good sterilization effect, and the compost product can be used as organic fertilizer, but the treatment cycle is often as long as several months, and a large amount of malodorous gas will be generated during the treatment process.

Method used

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  • Biological enzyme prepared from pigs died of diseases and preparation method thereof
  • Biological enzyme prepared from pigs died of diseases and preparation method thereof
  • Biological enzyme prepared from pigs died of diseases and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one: microbial screening in composite bacterial agent

[0047] 1. The selection of microorganisms in the compound microbial agent A.

[0048] (1) Screening for protein degradation ability. Set up 8 treatment groups, and in each group, use a meat grinder to crush the lean meat of sick and dead pigs to 50 mesh, add sterilized water according to the mass ratio of pork and water at 1:1, and use high temperature and high pressure to sterilize the crushed materials at 133 °C. Bacteria treatment for 90min. Centrifuge at 10,000 rpm at 4°C, remove the fat solids in the upper part, and make the remaining part into a 200g system, and then sterilize at 121°C for 20min to obtain pork culture medium. Add Bacillus amyloliquefaciens ( figure 1 Bacteria 1), Bacillus thuringiensis ( figure 1 Bacteria 2), Paenibacillus polymyxa ( figure 1 Bacteria 3), Bacillus subtilis ( figure 1 Bacteria 4), Pseudomonas aeruginosa ( figure 1 Bacteria 5), ​​Saccharomyces cerevisiae ( fi...

Embodiment 2

[0062] Example 2: Preparation and effect display of microbial compound bacterial agent

[0063] 1. Prepare compound microbial agent A. Each microorganism in the compound microbial agent A was activated and cultured with beef extract peptone medium until the concentration of viable bacteria was 3×10 8 CFU / mL. Composite microbial agent A is configured in the manner shown in Table 3. Each numerical value in Table 2 is the volume ratio (i.e. live bacteria ratio) of each microorganism bacterium liquid; For example, group 4 refers to by bacterium liquid volume ratio (or by live bacteria ratio), Pseudomonas aeruginosa: Bacillus amyloliquefaciens: subtilis Bacillus = 1:2:2. Among them, the commercially purchased Pseudomonas aeruginosa comes from Shanghai Fuxiang Biotechnology Co., Ltd. (ATCC15442); the commercially purchased Bacillus amyloliquefaciens comes from Weifang Ruichen Biotechnology Co., Ltd.; the commercially purchased Bacillus subtilis comes from Shanghai Fuxiang Biotech...

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Abstract

The invention belongs to the field of microbial complex microbial inoculants, and particularly relates to an enzyme for biological fermentation of pigs died of diseases and a preparation method thereof. According to the specific technical scheme, dead animals serve as fermentation substrates, aerobic fermentation is conducted for 3-5 days firstly, and then anaerobic fermentation is conducted for 3-5 days. The fermentation process provided by the invention is simple to operate, low in energy consumption, safe in product and free of secondary pollution, realizes recycling of resources on the basis of harmless treatment of the pigs died of diseases, and has a very good popularization prospect.

Description

technical field [0001] The invention belongs to the field of microbial compound bacterial agents, and in particular relates to a biological enzyme prepared by using sick and dead pigs and a preparation method thereof. Background technique [0002] In pig breeding, the mortality rate of pigs is about 5% to 10%. Faced with such a large number of sick and dead pigs, if timely, effective and scientific treatment is not carried out, it may cause the spread of the disease, which will affect the public health environment and the safety belt of livestock and poultry. a serious threat. [0003] The harmless treatment of sick and dead pigs refers to the process of decomposing the dead pig carcasses and killing the viruses carried by the pigs themselves through physical, chemical or biological methods. The methods commonly used in my country at this stage include: 1. Deep burial. This method is the most commonly used, using soil to isolate and prevent the spread of the virus, and usi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F1/00C05F17/10C05F17/20C05F17/50
CPCC05F1/00C05F17/10C05F17/20C05F17/50Y02A40/20Y02W30/40
Inventor 闫志英蒋心茹何光赞吕青阳毕列锋许力山张云祥高胜黄亮唐颖玲
Owner SICHUAN JIENENG DRYING EQUIP CO LTD
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