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Method for detecting new coronavirus SARS-CoV-2 antigen by combining two-dimensional plane with SERS (Surface Enhanced Raman Scattering)

A two-dimensional plane, sars-cov-2 technology, applied in the direction of measuring devices, material excitation analysis, instruments, etc., can solve the urgent need to improve the sensitivity and specificity of antigen detection, achieve fast detection speed, good stability, preparation low cost effect

Pending Publication Date: 2022-07-12
XIAMEN UNIV
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Problems solved by technology

However, the sensitivity and specificity of antigen detection urgently need to be improved

Method used

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  • Method for detecting new coronavirus SARS-CoV-2 antigen by combining two-dimensional plane with SERS (Surface Enhanced Raman Scattering)
  • Method for detecting new coronavirus SARS-CoV-2 antigen by combining two-dimensional plane with SERS (Surface Enhanced Raman Scattering)

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Embodiment Construction

[0036] In order to explore the feasibility of detecting the novel coronavirus SARS-CoV-2 virus antigen, pseudotyped novel coronavirus SARS-CoV-2 virus (PSV) purchased from Fubaiao (Suzhou) Biomedical Technology Co., Ltd. -2S protein lentivirus as a target. This PSV, which has been widely used to mimic SARSCoV-2 pseudovirion, is a retrovirus that integrates the envelope glycoprotein of another different virus, thereby forming an envelope with an exogenous virus, while A virus whose genome retains the genome characteristics of the retrovirus itself. The surface of the virus envelope contains the spike glycoprotein (S protein) of the new coronavirus, which can simulate the new coronavirus particle.

[0037] 1) Preparation of nano-gold sol: take 200 mL of chloroauric acid solution with a mass fraction of 0.01% and heat it to boiling under magnetic stirring; then take 4.5 mL of a 1% sodium citrate solution and add it to the boiling chloroauric acid solution , and the reaction was ...

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Abstract

The invention discloses a method for detecting a new coronavirus SARS-CoV-2 antigen by combining a two-dimensional plane with SERS (Surface Enhanced Raman Scattering), and belongs to the technical field of biology. According to the detection method disclosed by the invention, the principle of specific recognition of a new coronavirus SARS-CoV-2S protein-nucleic acid aptamer is utilized, after specific immunization is carried out on S protein and a two-dimensional plane of immune gold nanoparticles attached with the new coronavirus SARS-CoV-2S protein, the immune gold nanoparticles are combined with immune gold signal nanoparticles, and a sandwich structure is constructed; then, the SERS signal can be directly detected through a portable Raman spectrometer, and the ultrahigh detection sensitivity of the SARS-CoV-2S protein as low as 1TU / ml to the new coronavirus SARS-CoV-2S protein is realized. According to the method, nucleic acid extraction or antibody-dependent detection is not needed, the novel coronavirus SARS-CoV-2 protein can be specifically and ultrasensitively detected in a short time, and a favorable and effective way is created for early screening of pathogens infected with the novel coronavirus SARS-CoV-2S.

Description

technical field [0001] The invention relates to a method for detecting a novel coronavirus SARS-CoV-2 antigen in combination with a two-dimensional plane and SERS. Background technique [0002] The global pandemic of the novel coronavirus pneumonia epidemic has had a major impact on global public health, social and economic operations. When drug development is delayed and the effectiveness of vaccines has not been fully verified, large-scale rapid screening of the population is carried out to find potential infected persons (especially mild and asymptomatic patients), and centralized isolation is carried out to cut off the transmission route And protecting vulnerable populations is a top priority. Therefore, early diagnosis is particularly important for the new coronavirus SARS-CoV-2 infection. [0003] The clinical diagnosis of SARS-CoV-2 relies on previous exposure history, clinical manifestations and laboratory tests, including swab nucleic acid / antigen testing, serolog...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N21/01
CPCG01N21/658G01N21/01
Inventor 李剑锋关鹏程顾曼曼
Owner XIAMEN UNIV
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