Preparation method of 2-acetylqueloside A reference substance

A technology of reference substance, acetyl, which is applied in the field of preparation of 2-Acetyroside A reference substance, can solve problems such as the influence of extraction rate without mentioning it, and achieve high reuse rate, easy crystallization, and high extraction rate Effect

Active Publication Date: 2022-07-22
成都格利普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The literature does not mention the influence of other extraction parameters and extraction methods on the extraction rate of 2-acetyl wanglioliside A

Method used

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  • Preparation method of 2-acetylqueloside A reference substance
  • Preparation method of 2-acetylqueloside A reference substance
  • Preparation method of 2-acetylqueloside A reference substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A preparation method of 2-acetyl lilyside A reference substance, comprising the following steps: step 1, extracting lily liquid; immersing lily bulbs, filtering, and combining the extracts to obtain a lily extract. In step 2, the crude product of 2-acetyl lilyside A is prepared; the lily extract obtained in step 1 is subjected to centrifugation, polymer adsorption chromatography, gradient elution and concentration to obtain the crude product of 2-acetyl lilyside A. Step 3, the preparation of 2-Acetyl Lilily glucoside A crude product is separated and purified by liquid phase; the crude 2-Acetyl Lily glucoside A obtained in Step 2 is dissolved, filtered through a microporous membrane, prepared by liquid phase separation and purification, concentrated and crystallized. 2-Acetyl lilyside A crude crystals were obtained. Step 4, acquisition of 2-acetyl lilyside A; 2-acetyl lilyside A is obtained by dissolving and standing for crystallization.

[0066] The steps of lily extra...

Embodiment 2

[0076] refer to Figure 1-Figure 10 As shown in , a preparation method of 2-acetyl lilyside A reference substance includes the following steps: step 1, extracting lily liquid; immersing lily bulbs, filtering, and combining the extracts, and obtaining a lily extract. In step 2, the crude product of 2-acetyl lilyside A is prepared; the lily extract obtained in step 1 is subjected to centrifugation, polymer adsorption chromatography, gradient elution and concentration to obtain the crude product of 2-acetyl lilyside A. Step 3, the preparation of 2-Acetyl Lilily glucoside A crude product is separated and purified by liquid phase; the crude 2-Acetyl Lily glucoside A obtained in Step 2 is dissolved, filtered through a microporous membrane, prepared by liquid phase separation and purification, concentrated and crystallized. 2-Acetyl lilyside A crude crystals were obtained. Step 4, acquisition of 2-acetyl lilyside A; 2-acetyl lilyside A is obtained by dissolving and standing for crys...

Embodiment 3

[0087] A preparation method of 2-acetyl lilyside A reference substance, comprising the following steps: step 1, extracting lily liquid; immersing lily bulbs, filtering, and combining the extracts to obtain a lily extract. In step 2, the crude product of 2-acetyl lilyside A is prepared; the lily extract obtained in step 1 is subjected to centrifugation, polymer adsorption chromatography, gradient elution and concentration to obtain the crude product of 2-acetyl lilyside A. Step 3, the preparation of 2-Acetyl Lilily glucoside A crude product is separated and purified by liquid phase; the crude 2-Acetyl Lily glucoside A obtained in Step 2 is dissolved, filtered through a microporous membrane, prepared by liquid phase separation and purification, concentrated and crystallized. 2-Acetyl lilyside A crude crystals were obtained. Step 4, acquisition of 2-acetyl lilyside A; 2-acetyl lilyside A is obtained by dissolving and standing for crystallization.

[0088] The steps of lily extra...

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Abstract

The invention relates to a preparation method of a 2-acetylqueen lily glycoside A reference substance. The preparation method comprises the following steps: step 1, extracting lily liquid; lilium brownii bulbs are subjected to warm soaking and filtering, extracting solutions are combined, and a lilium brownii extracting solution is obtained; step 2, preparing a crude product of the 2-acetyl bulbus lilii glycoside A; 2, performing centrifugation, polymer adsorption chromatography, gradient elution and concentration on the lily extracting solution obtained in the step 1 to obtain a crude product of 2-acetylqueen lily glycoside A; step 3, preparing a crude product of the 2-acetyl bulbus lilii glycoside A, and separating and purifying a liquid phase; 2, dissolving the 2-acetyl bulbus lilioside A crude product obtained in the step 2, filtering by a microporous filter membrane, separating and purifying a prepared liquid phase, concentrating, and crystallizing to obtain a 2-acetyl bulbus lilioside A crude crystal; step 4, obtaining the 2-acetyl bulbus lilii glycoside A; and dissolving, standing and crystallizing to obtain the 2-acetyl king lily glycoside A. The overall extraction rate of the 2-acetylqueen lily glycoside A can be improved.

Description

technical field [0001] The invention relates to the field of reference substance preparation, in particular to a preparation method of a 2-acetyl lilyside A reference substance. Background technique [0002] Lily has the same origin as medicine and food, 10 varieties are edible, and 3 varieties are used for medicinal purposes, namely Lilium lancifolium Thunb., Lilium brownii F.E.Brown var. viridulum Baker, Lilium Lilium Dried fleshy scale leaves of pumilum DC. Its edible and medicinal parts are the bulbs of lily plants. Lily bulbs contain saponins, alkaloids, polysaccharides, phenolic glycerides, amino acids and other components. Lily phenolic acid glycerolipid compounds, including 1-O-p-coumaroyl-2-O feruloyl glyceride, lilyside A, lilyside B, lilyside C, lilyside D, lilyside E. Lily glucoside F, has anti-inflammatory, antioxidant and anti-viral activities (Luo Linming. Research progress on chemical constituents and pharmacological effects of Lily of traditional Chinese ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/06
CPCC07H1/08C07H15/06
Inventor 冉波阎志鹏黎先智张付先吴明春颜洪荣
Owner 成都格利普生物科技有限公司
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