The invention belongs to the field of
probiotic microcapsule preparations, and particularly relates to a
probiotic microcapsule preparation based on a water-in-water
emulsion structure and a preparation method thereof. According to the method, a water-phase continuous phase in which a particle stabilizer is dispersed and a water-phase dispersed phase containing probiotics are subjected to shear emulsification, a stable water-in-water
Pickering emulsion loaded with the probiotics is prepared, and the
probiotic microcapsule preparation can be obtained after the
emulsion loaded with the probiotics is dried. According to the water-in-water
emulsion system constructed by the method, an oil-water interface barrier existing in a traditional oil-water emulsion
system is broken through, free migration of water in the
drying process after embedding of the probiotics is broken through, combination of embedding and
drying of the water-in-water
Pickering emulsion is achieved, and particularly, the probiotic
solid preparation can be directly prepared through
spray drying. According to the prepared probiotic preparation, the tolerance of probiotics is improved, and the
survival rate can still reach 97% or above after the probiotic preparation is stored for six months under the normal temperature condition.