Preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases

A technique for cardiovascular and cerebrovascular diseases and dihydroquercetin, which is applied in the field of preparation of dihydroquercetin for anti-cancer, anti-oxidation, and cardiovascular and cerebrovascular diseases, can solve the problem that the purity is difficult to accurately determine, the extraction rate is low, and the dihydroquercetin issues such as differences in the purity of

Pending Publication Date: 2022-07-22
沈阳市丽晨生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In related technologies, there are certain differences in the purity of dihydroquercetin extracted under

Method used

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  • Preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases

Examples

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no. 1 example

[0030] The preparation method of Phellinus linteus extract provided in the first embodiment of the present invention comprises:

[0031] Provide larch powder with a particle size of 0.12nm and mix with deionized water at a ratio of 1:15 to obtain a mixed solution;

[0032] The enzyme with cellulase and the mixed solution were mixed with enzymolysis for 2 hours, and added to a centrifuge to be centrifuged at a rotational speed of 6000 r / min for 6 minutes;

[0033] After the centrifugation is completed, the supernatant is collected to obtain a larch enzymatic hydrolysate;

[0034] After the larch enzymatic hydrolysis solution and the 81% concentration ethanol solution are mixed in a ratio of 1:4, the obtained mixed solution is placed in an ultrasonic extractor for extraction for 67 minutes to obtain an extract;

[0035] Put the extract into the autoclave and pressurize it to 550Mpa;

[0036] After the extract was kept under pressure for 12 minutes, the pressure was reduced, an...

no. 2 example

[0041] The extraction method of dihydroquercetin provided in the second embodiment of the present invention comprises:

[0042] Provide larch powder with a particle size of 0.14nm and mix with deionized water at a ratio of 1:15 to obtain a mixed solution;

[0043] The enzyme with cellulase and the mixed solution were mixed with enzymolysis for 2 hours, and added to a centrifuge to be centrifuged at a rotational speed of 6000 r / min for 8 minutes;

[0044] After the centrifugation is completed, the supernatant is collected to obtain a larch enzymatic hydrolysate;

[0045] After the larch enzymolysis solution and the 81% concentration ethanol solution are mixed at a ratio of 2:9, the obtained mixed solution is placed in an ultrasonic extractor for extraction for 80 minutes to obtain an extract;

[0046] Put the extract into the autoclave and pressurize it to 570Mpa;

[0047] After the extract was kept under pressure for 12 minutes, the pressure was reduced, and the temperature ...

no. 3 example

[0052] The extraction method of dihydroquercetin provided in the third embodiment of the present invention comprises:

[0053] Provide larch powder with a particle size of 0.11nm and mix with deionized water at a ratio of 1:15 to obtain a mixed solution;

[0054] The enzyme with cellulase and the mixed solution were mixed with enzymolysis for 2 hours, and added to a centrifuge to be centrifuged at a rotational speed of 6000 r / min for 8 minutes;

[0055] After the centrifugation is completed, the supernatant is collected to obtain a larch enzymatic hydrolysate;

[0056] After using the larch enzymatic hydrolysis solution and the 81% concentration ethanol solution to mix at a ratio of 3:10, the obtained mixed solution is placed in an ultrasonic extractor for extraction for 75 minutes to obtain an extract;

[0057] Put the extract into the autoclave and pressurize it to 570Mpa;

[0058] After the extract was kept under pressure for 12 minutes, the pressure was reduced, and the ...

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Abstract

The invention discloses a preparation method of dihydroquercetin for resisting cancer, oxidation and cardiovascular and cerebrovascular diseases, which comprises the following steps: firstly, carrying out mixed enzymolysis on enzyme with cellulase and larch powder with predetermined granularity to obtain larch enzymatic hydrolysate; mixing the larch enzymatic hydrolysate and an ethanol solution with the concentration of 81% according to the proportion of (1-5): (3-10), and extracting the obtained mixed solution in an ultrasonic extraction instrument for 62-100 minutes to obtain extract liquor; then putting the extract liquor into a high-pressure kettle, pressurizing and heating, and crystallizing the extract liquor to obtain dihydroquercetin; and finally, measuring the crystallized dihydroquercetin through an infrared spectrum, and screening the dihydroquercetin with the purity of more than 90% to obtain a refined dihydroquercetin product. Therefore, the purity of dihydroquercetin extracted under different conditions can be accurately determined, the extraction rate is further improved, and the purity can be accurately determined.

Description

technical field [0001] The invention relates to the technical field of dihydroquercetin, in particular to a method for preparing dihydroquercetin for anti-cancer, anti-oxidation, and cardiovascular and cerebrovascular diseases. Background technique [0002] Dihydroquercetin is a kind of natural medicine with significant antioxidant effect. It can be widely used in medicines and health functional foods as the main and auxiliary raw materials of medicine and food bioactive additives. It is widely used in medicine, industry and agriculture. Dihydroquercetin belongs to dihydroflavonoids, vitamin P group, is a strong antioxidant, the adsorption of free radicals in natural antioxidants can reach 32μmol / mg (dihydroquercetin purity 95%), dihydroquercetin As a natural antioxidant, hydroquercetin plays a significant role in scavenging free radicals and prolonging cell life. It can be expected that dihydroquercetin has strong biological activity and medicinal value. In the related ar...

Claims

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Application Information

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IPC IPC(8): C12P17/06C07D311/32C07D311/40
CPCC12P17/06C07D311/32C07D311/40
Inventor 刘丽丽
Owner 沈阳市丽晨生物医药科技有限公司
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