65 results about "Vasodilator agents" patented technology

The invention provides a mesenchymal stem cell preparation for promoting knee joint repair as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading transforming growth factor-beta1 and interleukin-6 on chitosan microspheres, then preparing a chitosan-oxidized starch-carboxymethyl-beta-cyclodextrin bracket loaded with sustained release microspheres, loading luteolin and quercetin, carrying out mixed culture with mesenchymal stem cells, adding a traditional Chinese medicine extract, and carrying out freeze-drying to obtain the luteolin-quercetin-modified mesenchymal stem cells. The mesenchymal stem cell preparation for promoting knee joint repair is prepared. The preparation method of the carbon anode plate for fluorine preparation is simple, the generation of polarization of the carbon anode plate is prevented, and the carbon anode plate has the advantages of low porosity, low ash content, high mechanical strength, high volume density, obviously prolonged service life, low resistivity and the like, and has a wide application prospect.

The invention aims to provide a preparation method and application of a metal-organic nano complex for combined treatment of tumors, belongs to the technical field of antitumor drugs, and adopts a one-pot method to prepare copper-quercetin nanoparticles with uniform morphology. The obtained metal organic complex CQT nanoparticles can be used for X-ray induced sensitization radiotherapy / chemical power combined treatment to promote tumor cell apoptosis, meanwhile, due to introduction of copper ions, the steady state of the copper ions in tumor cells is broken, tumor cell copper death is induced, interaction is formed between copper death and the radiotherapy biological effect, and the tumor cell apoptosis is promoted. The synergistic tumor treatment effect is realized. The novel CQT nano complex for three-in-one treatment of sensitization radiotherapy / chemodynamic therapy / copper death has the function of obviously delaying the growth speed of tumors. The system can maintain good stability and biocompatibility, provides an innovative and efficient treatment potential scheme for intractable tumors clinically lacking specific targets, and improves the long-term prognosis of early metastatic tumors.

The present invention provides an enhanced NK cell, its preparation method and application, belonging to the field of biomedical technology. The present invention uses a compound of quercetin, paclitaxel and resveratrol as a culture medium induction additive to pre-treat NK cells, successfully enhancing the killing toxicity of NK cells against human lung cancer cells and effectively inhibiting tumor growth, opening up a new way for the treatment of lung cancer and having important research value and potential clinical application prospects in the field of tumor immunotherapy.

The invention discloses a nucleic acid tetrahedron DNA-quercetin compound and application of the nucleic acid tetrahedron DNA-quercetin compound in treatment of retinal neovascular diseases. In the nucleic acid tetrahedron DNA-quercetin compound disclosed by the invention, a quercetin molecule is connected to one single-stranded DNA of four single-stranded DNAs of a nucleic acid tetrahedron through an azide coupling reaction. The compound is stable in physical property, quantitatively carried quercetin molecules guarantee the stability of the treatment effect of the compound, generation of new vessels can be effectively prevented, remodeling of retina non-perfusion area vessels can be promoted, retina vascular injury can be recovered, a new strategy is provided for treatment and prevention of retina neovascular diseases, and the compound is worthy of popularization and application. Meanwhile, compared with tFNAs-QUE obtained through an existing electrostatic adsorption method, the tFNAs-QUE synthesized through the azide coupling method has a better treatment effect, pathological neovascularization is greatly reduced, and meanwhile generation of physiological blood vessels in a non-vascular region is obviously promoted.

The invention provides an application of dihydroquercetin in preparation of an antidepressant drug. The dihydroquercetin is found to be capable of effectively improving the depressive symptom induced by IBD for the first time, and compared with a traditional treatment method, the medicine (TAX) shows a more excellent effect in the aspect of improving the depressive symptom of IBD mice. Through behavioral tests, such as a forced swimming test and an open-field test, the TAX obviously reduces the immobility time and the floating time of a mouse under a medium dose (50mg / kg), and shows an obvious anti-depression effect. Therefore, the dihydroquercetin has a great application prospect in preparation of the antidepressant drugs.

The invention discloses a traditional Chinese medicine composition for treating chronic obstructive pulmonary disease as well as a preparation and application thereof, and belongs to the technical field of chronic obstructive pulmonary disease medicines. The traditional Chinese medicine composition consists of a qi tonifying component, a blood activating component and a phlegm reducing component, or consists of the qi tonifying component and the blood activating component, or consists of the qi tonifying component and the phlegm reducing component, wherein the qi-tonifying component is selected from any one or a combination of more of ginsenoside Rg1, ginsenoside Rb1, astragaloside and schizandrin; the blood activating component is selected from any one or combination of more of ligustrazine and ferulic acid; the phlegm reducing component is selected from any one or combination of more of glucosinolate and quercetin. The compatibility and combination of the medicines have obvious effects in the aspects of improving lung inflammatory response and inhibiting pathological remodeling of pulmonary blood vessels.

The invention relates to the technical field of traditional Chinese medicine preparation, and discloses a production process for clearing away lung-heat, nourishing lung and removing nodules, which comprises the following steps: classifying and pretreating medicinal materials, namely dividing the medicinal materials into a low-temperature extraction group, a medium-temperature extraction group and a high-temperature extraction group according to chemical properties of components, crushing the medicinal materials to 60-80 meshes, and mixing the medicinal materials according to the ratio of the weight of the medicinal materials to the volume of water of 1: 6-1: 10, and soaking for 1-2 hours at the temperature of 10-20 DEG C. The invention further provides a formula for clearing away the lung-heat, nourishing the lung and removing nodules, and the medicine comprises a lung-heat-clearing and detoxifying group, a drug delivery group, a drug delivery group and a drug delivery group, the lung moistening and nourishing group comprises lily, radix ophiopogonis and bulbus fritillariae cirrhosae; and the phlegm-reducing and stagnation-resolving group comprises bromelain, polyunsaturated phosphatidylcholine and kelp. By optimizing an extraction process, multi-step separation and purification, a dynamic balance technology and a granulation preparation process, the comprehensive effects of high extraction efficiency, excellent component purity, high preparation stability and remarkable curative effect are achieved.

The present disclosure provides a preparation method of a mesenchymal stem cell (MSC) and an application thereof in a knee product. The additive composition including NOG and quercetin provided by the present disclosure includes components that are combined scientifically and reasonably and play a synergistic role, and has a stable efficacy.

The invention discloses an oxidative stress response nano preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing sodium cocoyl glutamate and 2-amino-2-methyl-1-propanol with water, slowly dropwise adding isopropyl triisopropoxy silane, adding a mixed solution of N-(3-trimethoxysilylpropyl) urea, sodium selenide and isopropyl triisopropoxy silane, and stirring to form nanoparticles; (2) dispersing the nano-particles in a buffer solution containing lysozyme and esterase, and oscillating and incubating to obtain organic silicon nano-particles with hollow structures; and (3) dispersing the organic silicon nanoparticles in a dihydroquercetin solution, filtering, washing uncombined substances, and carrying out vacuum drying to obtain the oxidative stress response nano preparation. The preparation disclosed by the invention can trigger drug release through ROS in an oxidative stress environment, so that the targeting property of the drug in pathological tissues such as tumors and the treatment effect of the drug are improved, the influence on normal tissues is reduced, and the treatment safety of the drug is enhanced.

The invention belongs to the field of biological medicine, and relates to a preparation method of a dihydroquercetin preparation and application of the dihydroquercetin preparation in bedsore treatment, and the method comprises the following specific steps: dissolving ferric trichloride hexahydrate in methanol, uniformly mixing, carrying out ultrasonic treatment, dropwise adding into a polyvinylpyrrolidone methanol solution, and uniformly mixing to obtain a mixed solution; dissolving dihydroquercetin in methanol, carrying out ultrasonic treatment, dropwise adding into the mixed solution, reacting at room temperature, dialyzing the reacted solution in a dialysis bag, and freeze-drying the dialyzed solution to obtain iron-dihydroquercetin nanoparticles; the preparation method comprises the following steps: dissolving iron-dihydroquercetin nanoparticles in an oxidized dextran aqueous solution, adding H2O2, and then mixing with an aminated gelatin aqueous solution to obtain the FTH-NO hydrogel. The hydrogel is good in photo-thermal stability, has good self-repairing performance, swelling property and degradation effect, has a good hemostatic effect, can accelerate healing of bedsores, and provides a new strategy for treatment of bedsore wounds, and the bacteriostasis rate of the hydrogel to escherichia coli and staphylococcus aureus is higher than 98%.

The invention provides an enhanced NK cell as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Quercetin, paclitaxel and resveratrol are compounded to serve as a culture medium induction additive to pretreat the NK cells, the killing toxicity of the NK cells on human lung cancer cells is successfully improved, tumor growth is effectively inhibited, a new way is opened up for lung cancer treatment, and the method has a wide application prospect. The invention has important research value and potential clinical application prospect in the field of tumor immunotherapy.

The invention discloses a method, a system and equipment for evaluating the treatment effect of an anti-aging drug Senlytics. According to the invention, it is found for the first time that the anti-aging drug Senlytics composed of dasatinib and quercetin plays an anti-aging role by inhibiting the level of JPX, BRD4, p-p65 / p65 and / or JPX-BRD4-p65 compounds, and on the basis of this, the anti-aging effect of the anti-aging drug Senlytics composed of dasatinib and quercetin is achieved. The invention provides a method, a system and equipment for evaluating the treatment effect of an anti-aging drug Senlytics composed of dasatinib and quercetin based on the expression level of JPX, BRD4, p-p65 / p65 and / or JPX-BRD4-p65 compounds for the field, and the method, the system and the equipment have wide application prospects in the aspect of evaluation of the treatment effect of cardiovascular disease drugs.

The invention discloses an application of 8-methyl quercetin in preparation of a TLR7 (toll-like receptor 7) immunomodulator. According to the invention, it is found that 8-methyl quercetin can be used as a ligand of TLR7, and the 8-methyl quercetin has strong binding and interaction with swine TLR7 protein. Therefore, the 8-methyl quercetin can be used as an immunomodulator or a lead compound acting on the TLR7. As the TLR7 protein is conservative in evolution, the application of the 8-methyl quercetin is not limited to pigs, and the 8-methyl quercetin can also be applied to other species.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a drug delivery carrier based on dunaliella salina cells, and the method comprises the following steps: selecting dunaliella salina for standardized culture to obtain dunaliella salina living cells; the target drug and the dunaliella salina living cells are loaded into the dunaliella salina living cells through an endocytosis method; free drug molecules are removed through centrifugation, and the dunaliella salina cell drug delivery carrier is obtained. Efficient drug loading is achieved by activating an endocytosis pathway, the highest quercetin loading rate reaches 92%, and the quercetin loading rate is remarkably superior to that of a co-incubation method and an electroporation method; the dunaliella salina cell carrier is excellent in biocompatibility, and the cell survival rate is gt; no organ toxicity exists in a mouse experiment; the medicine can be slowly released in intestinal tracts, the retention time is prolonged, the anti-tumor effect in a colorectal cancer model is remarkable, and the immunoregulation effect can be enhanced; the preparation process is simple and controllable, special equipment is not needed, and a new scheme is provided for constructing an efficient delivery system for natural drugs.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

The present application relates to the technical field of skin mucosa repair, and particularly relates to a pharmaceutical preparation for repairing skin mucosa injury. The preparation takes kaempferol, quercetin, 3-p-coumaroyl-1, 4, 6'-triacetyl sucrose, 3-p-coumaroyl-2', 3', 6'-triacetyl sucrose, (+)-syringaresinol and vitamin B12 extracted from Prunus tomentosa as active ingredients, so as to solve the problems of poor curative effect, long repair period and easy scarring of the existing skin mucosa injury repair preparation, and realize efficient, safe and high-quality skin mucosa injury repair effect.

The invention relates to the technical field of senescence delaying medicines, and discloses a senescence delaying preparation which comprises the following components: resveratrol, dihydroquercetin, curcumin, coenzyme Q10, bromelain, glutathione, roxburgh rose SOD and nicotinamide ribose NR. A preparation method of the preparation capable of delaying senescence comprises the following steps: step 1, weighing the resveratrol, the dihydroquercetin, the curcumin, the coenzyme Q10, the bromelain, the glutathione, the roxburgh rose SOD and the nicotinamide ribose NR in parts by weight for later use; and 2, mixing the raw materials, and further grinding. Compared with the prior art, the invention has the following advantages: experiments prove that after the product is taken for 10 weeks, the human body is kept in a vigorous state, vigorous energy, full brain and relatively vigorous state, age pigments and pigmented spots are gradually eliminated, the skin is in a whitening state, the immune function of the human body can be improved, and an excellent anti-aging effect can be shown.

The invention relates to a double-layer buccal tablet for preventing and treating oral ulcer and a preparation method of the double-layer buccal tablet. The double-layer buccal tablet is prepared from a quick release layer containing isoquercetin and a slow release layer containing selenium polysaccharide, wherein the weight part ratio of the dosage of the isoquercetin to the dosage of the selenium-containing polysaccharide is (7.5-20): (17.5-5), and the total weight part of the dosage of the isoquercetin to the dosage of the selenium-containing polysaccharide is 25. By controlling the quick release of the isoquercetin, the use amount of the selenium polysaccharide and the release sequence of slow release adhesion, the selenium polysaccharide forms an adhesion layer on the wound surface pretreated by the isoquercetin, so that the advantages of improving the curative effect on the oral ulcer, regulating the balance of oral flora and preventing the recurrence of the oral ulcer can be realized.

The present invention belongs to the field of biopharmaceutical technology and specifically relates to a notoginsenoside Fe composition and its use in preparing PTGS2 protein inhibitors and drugs for preventing and treating PTGS2 diseases. The notoginsenoside Fe composition provided by the present invention is composed of notoginsenoside Fe, ginsenoside Rg5, ginsenoside Rk1, quercetin, and eriodictyol. The present invention is the first to discover a novel PTGS2 inhibitor, notoginsenoside Fe, and prepare it into a notoginsenoside Fe composition, providing a new solution for the research and development of drugs for preventing and treating PTGS2-related diseases. The notoginsenoside Fe composition provided by the present invention can reduce the levels of inflammatory factors in mice in colitis, gastritis, and arthritis models, improve colon length, and alleviate foot swelling.

The present invention discloses the use of luteolin, isoquercetin and febuxostat in the preparation of a medicament for preventing and / or treating hyperuricemia and gout. In the composition of luteolin and isoquercetin, the mass ratio of luteolin to isoquercetin is 1:5 to 100. In the solution of the present invention, it is found that luteolin and isoquercetin are used in combination with febuxostat in a specific ratio. On the one hand, it has the effects of inhibiting XOD oxidase and reducing uric acid, and on the other hand, it can antagonize the EP4 receptor to reduce inflammation. Therefore, the dosage of febuxostat can be reduced, the inflammatory factors during the occurrence of gouty arthritis can be inhibited, the acute attack of gouty arthritis can be relieved, and it has a significant effect on the treatment of gouty arthritis without obvious toxic and side effects.

The invention provides a vaginal packing with an antibacterial function and a preparation method and application thereof, and relates to the technical field of vaginal packing, the vaginal packing with the antibacterial function comprises dihydroquercetin, citric acid, galactooligosaccharide, isomaltooligosaccharide, sodium lactate, sodium hyaluronate and chitosan. The dihydroquercetin has a relatively strong antibacterial function; the permeation enhancer chitosan can prolong the residence time of the dihydroquercetin at the absorption part, improve the local concentration gradient and promote the vaginal mucosa to absorb the dihydroquercetin; sodium hyaluronate can improve the solubility and stability of dihydroquercetin, enhance the absorption efficiency of dihydroquercetin, further improve the antibacterial performance, control the release progress, realize slow release, accelerate the permeation of water into the interior and enhance the vaginal filling and imbibition speed. The isomaltooligosacharide and the sodium lactate can be mixed with the chitosan to form an adsorption material which is used for absorbing secretions, providing prebiotics support and adjusting the pH value.

The present invention discloses a dual-responsive quercetin-caffeic acid phenethyl ester lipid composite gel and its preparation method and application. The present invention first uses microemulsion-lipid cascade drug delivery technology to co-load quercetin and caffeic acid phenethyl ester into liposomes, and then distributes the liposomes in a dual-responsive gel composed of chitosan, poloxamer 407 and poly-N-isopropylacrylamide to construct a dual-responsive quercetin-caffeic acid phenethyl ester lipid composite gel. The composite gel has a temperature-sensitive and pH-sensitive dual-responsive performance, and after administration, quercetin can be preferentially released to achieve rapid anti-inflammatory effects, and then caffeic acid phenethyl ester is released to promote osteogenic differentiation in the periodontal part, which can synergistically play the therapeutic effects of eliminating periodontal tissue inflammation and promoting alveolar bone repair, and provides a new preparation for the treatment of periodontitis.

The present application relates to the field of biotechnology, in particular to astaxanthin and dihydroquercetin co-loaded liposome and its application in preparation of uric acid-lowering products. According to the steps of "in vitro function screening - optimal ratio determination - co-loaded liposome construction - surface coating and stabilization treatment - uric acid-lowering application verification", the obtained system is verified through physicochemical characterization, stability test and animal experiment. The results show that the co-loaded liposome can reduce the serum uric acid level of high uric acid model mice, inhibit the activity of liver xanthine oxidase, and improve the related indexes of kidney function and histopathological damage.

The application belongs to the technical field of biological medicine, and particularly relates to an anti-tumor composition, a medicine for preventing and / or treating tumors and application. The anti-tumor composition provided by the application comprises dihydroquercetin and beta-carotene; the dihydroquercetin and beta-carotene combined preparation can inhibit the proliferation and growth of various tumor cells of breast cancer, liver cancer, gastric cancer, prostate cancer, lung cancer and colorectal cancer, has an application prospect for preventing and / or treating various cancers, and meanwhile, taking breast cancer and colorectal cancer as examples, it is proved that the dihydroquercetin and beta-carotene can inhibit the tumor growth and metastasis of breast cancer and colorectal cancer. It can be seen that the composition provided by the application can significantly inhibit the proliferation, growth and metastasis of tumors, and can be used for preparing a medicine for preventing and / or treating primary and metastatic tumors.

This invention belongs to the field of pharmaceutical technology and discloses a cocrystal of dihydroquercetin and pyridinecarboxylic acid, its preparation method, composition, and uses. Specifically, this invention discloses a novel dihydroquercetin and pyridinecarboxylic acid cocrystal using dihydroquercetin (as shown in formula a) as the active pharmaceutical ingredient and pyridinecarboxylic acid (as shown in formula b) as the cocrystal ligand; a method for preparing the dihydroquercetin and pyridinecarboxylic acid cocrystal; and the application of the dihydroquercetin and pyridinecarboxylic acid cocrystal as the active pharmaceutical ingredient in the preparation of antioxidants, free radical scavengers, cell protectants, and antivirals.

The present application relates to a kind of double-layered lozenge for preventing and treating oral ulcer and its preparation method, the double-layered lozenge is prepared from immediate-release layer containing isoquercitrin and sustained-release layer containing selenium polysaccharide;Wherein, the weight ratio of the dosing amount of isoquercitrin and selenium polysaccharide is (7.5-20):(17.5-5), and the total weight of dosing amount is 25.The present application controls the dosage of isoquercitrin and selenium polysaccharide and the release sequence of sustained-release adhesion, forms an adhesion layer on the wound surface pretreated by isoquercitrin, can realize the advantages of improving the efficacy on oral ulcer, and adjusting oral flora balance, preventing the recurrence of oral ulcer.

The invention relates to an anti-cancer composition based on natural active ingredients and a medicine thereof, and belongs to the technical field of medicine compositions. The anticancer composition based on the natural active ingredients is prepared from the following components in percentage by mass: 1 to 10 percent of ginsenoside Rg3, 5 to 20 percent of dihydroquercetin, 20 to 40 percent of lucid ganoderma extract, 15 to 30 percent of astragalus extract and 15 to 30 percent of coix seed extract. In the anti-cancer composition based on the natural active ingredients, ginsenoside Rg3 has the effect of inhibiting tumor angiogenesis; the dihydroquercetin has remarkable antioxidant and anti-inflammatory activity; ganoderma lucidum polysaccharide can enhance the immune function of the body. Astragaloside has an immunoregulation effect; coix seed ester has the anti-tumor metastasis activity, the anti-cancer effect is remarkably enhanced through the synergistic effect of multiple components, toxic and side effects are low, the composition is suitable for long-term taking, and the product quality is stable.

The application discloses a preparation method of dihydroquercetin self-assembled nanoparticles and application of the nanoparticles in liver protection, and the preparation method of the nanoparticles is as follows: betaine and dihydroquercetin are respectively dissolved in methanol; the methanol solutions of the betaine and the dihydroquercetin are mixed in a 1:1 molar ratio, and the pH is adjusted to 7.0-7.5 by using a sodium hydroxide solution; the mixture is added into preheated PBS under stirring, sealed and kept at 55-65 DEG C for 20-40 minutes; after being cooled to room temperature, the mixture is subjected to ultra-pure water dialysis by using a dialysis bag, and the BTNPs, i.e. dihydroquercetin self-assembled nanoparticles, are obtained after freeze-drying. The nanoparticles have good ALI treatment effect, high safety, can simultaneously solve the defects of low solubility and poor bioavailability of dihydroquercetin, and the defects of betaine, such as easy occurrence of gastrointestinal adverse reactions and existence of dose-dependent safety risks; meanwhile, the nanoparticles can also regulate intestinal flora disorder caused by alcohol and prevent endotoxemia.

The invention discloses a quercetin pill for reducing hypertension by improving the vascular endothelial function, and relates to the technical field of quercetin pharmaceutical preparations. Comprising quercetin, eucommia ulmoides extract, hydroxypropyl-beta-cyclodextrin and hydroxypropyl methylcellulose. The quercetin pill can be used for preparing medicines for preventing and / or treating vascular endothelial dysfunction related cardiovascular diseases, especially hypertension closely related to a vascular endothelial injury mechanism. Quercetin promotes synthesis and release of nitric oxide of vascular endothelial cells, and NO is a powerful vasodilator and can diffuse to vascular smooth muscle cells, activate guanylate cyclase and increase cyclic guanosine monophosphate in the cells, so that the vascular smooth muscles are relaxed, blood vessels are expanded, and the vascular endothelial function is improved. In addition, quercetin can inhibit expression of inflammatory adhesion molecules and recruitment of inflammatory cells, so that inflammatory response of vascular endothelium is relieved. The problem that the use of quercetin in the aspect of reducing hypertension is limited in the prior art is solved.

The present application relates to a new use of Dihydroquercetin for preventing and treating cardiac toxicity induced by arsenic trioxide, and belongs to the technical field of medicine. In order to solve the problem that the prior art lacks effective treatment means for cardiac toxicity induced by ATO, the present application provides a new use of Dihydroquercetin for preventing and treating cardiac toxicity induced by arsenic trioxide, that is, the application of Dihydroquercetin in the preparation of a medicine for preventing and treating cardiac toxicity induced by arsenic trioxide. The present application proves through experiments that Dihydroquercetin can alleviate cardiac toxicity induced by ATO in multiple ways, including alleviating ATO-induced apoptosis, ferroptosis, oxidative stress, mitochondrial dysfunction and DNA damage. The present application finds that Dihydroquercetin has an excellent effect of preventing and treating cardiac toxicity induced by arsenic trioxide, provides a new strategy for improving the safety of arsenic trioxide, provides an embedding point for preparing a new medicine for preventing and treating cardiac toxicity induced by arsenic trioxide, and has an important clinical application prospect.