39 results about "Vasodilator agents" patented technology

The invention discloses an oxidative stress response nano preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing sodium cocoyl glutamate and 2-amino-2-methyl-1-propanol with water, slowly dropwise adding isopropyl triisopropoxy silane, adding a mixed solution of N-(3-trimethoxysilylpropyl) urea, sodium selenide and isopropyl triisopropoxy silane, and stirring to form nanoparticles; (2) dispersing the nano-particles in a buffer solution containing lysozyme and esterase, and oscillating and incubating to obtain organic silicon nano-particles with hollow structures; and (3) dispersing the organic silicon nanoparticles in a dihydroquercetin solution, filtering, washing uncombined substances, and carrying out vacuum drying to obtain the oxidative stress response nano preparation. The preparation disclosed by the invention can trigger drug release through ROS in an oxidative stress environment, so that the targeting property of the drug in pathological tissues such as tumors and the treatment effect of the drug are improved, the influence on normal tissues is reduced, and the treatment safety of the drug is enhanced.

The invention belongs to the field of biological medicine, and relates to a preparation method of a dihydroquercetin preparation and application of the dihydroquercetin preparation in bedsore treatment, and the method comprises the following specific steps: dissolving ferric trichloride hexahydrate in methanol, uniformly mixing, carrying out ultrasonic treatment, dropwise adding into a polyvinylpyrrolidone methanol solution, and uniformly mixing to obtain a mixed solution; dissolving dihydroquercetin in methanol, carrying out ultrasonic treatment, dropwise adding into the mixed solution, reacting at room temperature, dialyzing the reacted solution in a dialysis bag, and freeze-drying the dialyzed solution to obtain iron-dihydroquercetin nanoparticles; the preparation method comprises the following steps: dissolving iron-dihydroquercetin nanoparticles in an oxidized dextran aqueous solution, adding H2O2, and then mixing with an aminated gelatin aqueous solution to obtain the FTH-NO hydrogel. The hydrogel is good in photo-thermal stability, has good self-repairing performance, swelling property and degradation effect, has a good hemostatic effect, can accelerate healing of bedsores, and provides a new strategy for treatment of bedsore wounds, and the bacteriostasis rate of the hydrogel to escherichia coli and staphylococcus aureus is higher than 98%.

The invention discloses a method, a system and equipment for evaluating the treatment effect of an anti-aging drug Senlytics. According to the invention, it is found for the first time that the anti-aging drug Senlytics composed of dasatinib and quercetin plays an anti-aging role by inhibiting the level of JPX, BRD4, p-p65 / p65 and / or JPX-BRD4-p65 compounds, and on the basis of this, the anti-aging effect of the anti-aging drug Senlytics composed of dasatinib and quercetin is achieved. The invention provides a method, a system and equipment for evaluating the treatment effect of an anti-aging drug Senlytics composed of dasatinib and quercetin based on the expression level of JPX, BRD4, p-p65 / p65 and / or JPX-BRD4-p65 compounds for the field, and the method, the system and the equipment have wide application prospects in the aspect of evaluation of the treatment effect of cardiovascular disease drugs.

The invention discloses an application of 8-methyl quercetin in preparation of a TLR7 (toll-like receptor 7) immunomodulator. According to the invention, it is found that 8-methyl quercetin can be used as a ligand of TLR7, and the 8-methyl quercetin has strong binding and interaction with swine TLR7 protein. Therefore, the 8-methyl quercetin can be used as an immunomodulator or a lead compound acting on the TLR7. As the TLR7 protein is conservative in evolution, the application of the 8-methyl quercetin is not limited to pigs, and the 8-methyl quercetin can also be applied to other species.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a drug delivery carrier based on dunaliella salina cells, and the method comprises the following steps: selecting dunaliella salina for standardized culture to obtain dunaliella salina living cells; the target drug and the dunaliella salina living cells are loaded into the dunaliella salina living cells through an endocytosis method; free drug molecules are removed through centrifugation, and the dunaliella salina cell drug delivery carrier is obtained. Efficient drug loading is achieved by activating an endocytosis pathway, the highest quercetin loading rate reaches 92%, and the quercetin loading rate is remarkably superior to that of a co-incubation method and an electroporation method; the dunaliella salina cell carrier is excellent in biocompatibility, and the cell survival rate is gt; no organ toxicity exists in a mouse experiment; the medicine can be slowly released in intestinal tracts, the retention time is prolonged, the anti-tumor effect in a colorectal cancer model is remarkable, and the immunoregulation effect can be enhanced; the preparation process is simple and controllable, special equipment is not needed, and a new scheme is provided for constructing an efficient delivery system for natural drugs.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

The present application relates to the technical field of skin mucosa repair, and particularly relates to a pharmaceutical preparation for repairing skin mucosa injury. The preparation takes kaempferol, quercetin, 3-p-coumaroyl-1, 4, 6'-triacetyl sucrose, 3-p-coumaroyl-2', 3', 6'-triacetyl sucrose, (+)-syringaresinol and vitamin B12 extracted from Prunus tomentosa as active ingredients, so as to solve the problems of poor curative effect, long repair period and easy scarring of the existing skin mucosa injury repair preparation, and realize efficient, safe and high-quality skin mucosa injury repair effect.

The invention relates to a double-layer buccal tablet for preventing and treating oral ulcer and a preparation method of the double-layer buccal tablet. The double-layer buccal tablet is prepared from a quick release layer containing isoquercetin and a slow release layer containing selenium polysaccharide, wherein the weight part ratio of the dosage of the isoquercetin to the dosage of the selenium-containing polysaccharide is (7.5-20): (17.5-5), and the total weight part of the dosage of the isoquercetin to the dosage of the selenium-containing polysaccharide is 25. By controlling the quick release of the isoquercetin, the use amount of the selenium polysaccharide and the release sequence of slow release adhesion, the selenium polysaccharide forms an adhesion layer on the wound surface pretreated by the isoquercetin, so that the advantages of improving the curative effect on the oral ulcer, regulating the balance of oral flora and preventing the recurrence of the oral ulcer can be realized.

The present invention belongs to the field of biopharmaceutical technology and specifically relates to a notoginsenoside Fe composition and its use in preparing PTGS2 protein inhibitors and drugs for preventing and treating PTGS2 diseases. The notoginsenoside Fe composition provided by the present invention is composed of notoginsenoside Fe, ginsenoside Rg5, ginsenoside Rk1, quercetin, and eriodictyol. The present invention is the first to discover a novel PTGS2 inhibitor, notoginsenoside Fe, and prepare it into a notoginsenoside Fe composition, providing a new solution for the research and development of drugs for preventing and treating PTGS2-related diseases. The notoginsenoside Fe composition provided by the present invention can reduce the levels of inflammatory factors in mice in colitis, gastritis, and arthritis models, improve colon length, and alleviate foot swelling.

The present application relates to the field of biotechnology, in particular to astaxanthin and dihydroquercetin co-loaded liposome and its application in preparation of uric acid-lowering products. According to the steps of "in vitro function screening - optimal ratio determination - co-loaded liposome construction - surface coating and stabilization treatment - uric acid-lowering application verification", the obtained system is verified through physicochemical characterization, stability test and animal experiment. The results show that the co-loaded liposome can reduce the serum uric acid level of high uric acid model mice, inhibit the activity of liver xanthine oxidase, and improve the related indexes of kidney function and histopathological damage.

The application belongs to the technical field of biological medicine, and particularly relates to an anti-tumor composition, a medicine for preventing and / or treating tumors and application. The anti-tumor composition provided by the application comprises dihydroquercetin and beta-carotene; the dihydroquercetin and beta-carotene combined preparation can inhibit the proliferation and growth of various tumor cells of breast cancer, liver cancer, gastric cancer, prostate cancer, lung cancer and colorectal cancer, has an application prospect for preventing and / or treating various cancers, and meanwhile, taking breast cancer and colorectal cancer as examples, it is proved that the dihydroquercetin and beta-carotene can inhibit the tumor growth and metastasis of breast cancer and colorectal cancer. It can be seen that the composition provided by the application can significantly inhibit the proliferation, growth and metastasis of tumors, and can be used for preparing a medicine for preventing and / or treating primary and metastatic tumors.

This invention belongs to the field of pharmaceutical technology and discloses a cocrystal of dihydroquercetin and pyridinecarboxylic acid, its preparation method, composition, and uses. Specifically, this invention discloses a novel dihydroquercetin and pyridinecarboxylic acid cocrystal using dihydroquercetin (as shown in formula a) as the active pharmaceutical ingredient and pyridinecarboxylic acid (as shown in formula b) as the cocrystal ligand; a method for preparing the dihydroquercetin and pyridinecarboxylic acid cocrystal; and the application of the dihydroquercetin and pyridinecarboxylic acid cocrystal as the active pharmaceutical ingredient in the preparation of antioxidants, free radical scavengers, cell protectants, and antivirals.

The present application relates to a kind of double-layered lozenge for preventing and treating oral ulcer and its preparation method, the double-layered lozenge is prepared from immediate-release layer containing isoquercitrin and sustained-release layer containing selenium polysaccharide;Wherein, the weight ratio of the dosing amount of isoquercitrin and selenium polysaccharide is (7.5-20):(17.5-5), and the total weight of dosing amount is 25.The present application controls the dosage of isoquercitrin and selenium polysaccharide and the release sequence of sustained-release adhesion, forms an adhesion layer on the wound surface pretreated by isoquercitrin, can realize the advantages of improving the efficacy on oral ulcer, and adjusting oral flora balance, preventing the recurrence of oral ulcer.

The application discloses a preparation method of dihydroquercetin self-assembled nanoparticles and application of the nanoparticles in liver protection, and the preparation method of the nanoparticles is as follows: betaine and dihydroquercetin are respectively dissolved in methanol; the methanol solutions of the betaine and the dihydroquercetin are mixed in a 1:1 molar ratio, and the pH is adjusted to 7.0-7.5 by using a sodium hydroxide solution; the mixture is added into preheated PBS under stirring, sealed and kept at 55-65 DEG C for 20-40 minutes; after being cooled to room temperature, the mixture is subjected to ultra-pure water dialysis by using a dialysis bag, and the BTNPs, i.e. dihydroquercetin self-assembled nanoparticles, are obtained after freeze-drying. The nanoparticles have good ALI treatment effect, high safety, can simultaneously solve the defects of low solubility and poor bioavailability of dihydroquercetin, and the defects of betaine, such as easy occurrence of gastrointestinal adverse reactions and existence of dose-dependent safety risks; meanwhile, the nanoparticles can also regulate intestinal flora disorder caused by alcohol and prevent endotoxemia.

The invention discloses a quercetin pill for reducing hypertension by improving the vascular endothelial function, and relates to the technical field of quercetin pharmaceutical preparations. Comprising quercetin, eucommia ulmoides extract, hydroxypropyl-beta-cyclodextrin and hydroxypropyl methylcellulose. The quercetin pill can be used for preparing medicines for preventing and / or treating vascular endothelial dysfunction related cardiovascular diseases, especially hypertension closely related to a vascular endothelial injury mechanism. Quercetin promotes synthesis and release of nitric oxide of vascular endothelial cells, and NO is a powerful vasodilator and can diffuse to vascular smooth muscle cells, activate guanylate cyclase and increase cyclic guanosine monophosphate in the cells, so that the vascular smooth muscles are relaxed, blood vessels are expanded, and the vascular endothelial function is improved. In addition, quercetin can inhibit expression of inflammatory adhesion molecules and recruitment of inflammatory cells, so that inflammatory response of vascular endothelium is relieved. The problem that the use of quercetin in the aspect of reducing hypertension is limited in the prior art is solved.

The present application relates to a new use of Dihydroquercetin for preventing and treating cardiac toxicity induced by arsenic trioxide, and belongs to the technical field of medicine. In order to solve the problem that the prior art lacks effective treatment means for cardiac toxicity induced by ATO, the present application provides a new use of Dihydroquercetin for preventing and treating cardiac toxicity induced by arsenic trioxide, that is, the application of Dihydroquercetin in the preparation of a medicine for preventing and treating cardiac toxicity induced by arsenic trioxide. The present application proves through experiments that Dihydroquercetin can alleviate cardiac toxicity induced by ATO in multiple ways, including alleviating ATO-induced apoptosis, ferroptosis, oxidative stress, mitochondrial dysfunction and DNA damage. The present application finds that Dihydroquercetin has an excellent effect of preventing and treating cardiac toxicity induced by arsenic trioxide, provides a new strategy for improving the safety of arsenic trioxide, provides an embedding point for preparing a new medicine for preventing and treating cardiac toxicity induced by arsenic trioxide, and has an important clinical application prospect.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses an icodextrin preparation for peritoneal dialysis and a preparation method of the icodextrin preparation. According to the icodextrin preparation for peritoneal dialysis provided by the invention, the first independent solution and the second independent solution are respectively prepared by adopting a double-chamber structure, and the taurine, the quercetin and the hyperbranched polyglycerol are added into the first independent solution; the icodextrin peritoneal dialysis solution effectively solves the risks of metabolic disorder and peritoneal injury caused by a traditional glucose dialysis solution and peritoneal fibrosis and pipeline blockage existing in an existing icodextrin dialysis solution, the stability of the icodextrin peritoneal dialysis solution is remarkably improved, the probability of adverse reactions is reduced, and the icodextrin peritoneal dialysis solution is excellent in viscosity resistance and osmotic pressure stability and can be used for preparing peritoneal dialysis solutions. The peritoneal dialysis solution can well replace the existing peritoneal dialysis solution, is simple in preparation process and good in repeatability, is easy to realize industrial mass production, and has relatively high popularization and application values in peritoneal dialysis treatment of patients with end-stage nephropathy.

The invention provides a soothing composition for improving skin tolerance and application of the soothing composition. The composition is prepared from schizandrin A, dihydroquercetin, jonquil glucoside and panthenol. The raw materials in the composition cooperate with each other to synchronously intervene four major pathways of nerve-immunity-blood vessel-barrier, so that the vicious circle of sensitive skin is broken, and the comprehensive effect of quickly relieving symptoms to fundamentally improve the symptoms for a long time is realized.

The invention provides a preparation for promoting regeneration of skin fibroblasts and application of the preparation, and belongs to the technical field of cell biology. The preparation comprises two parts: a first culture medium for adherent culture, which contains resveratrol, melatonin, quercetin and other protective components; and the second culture medium is used for amplification and subculture and contains regeneration stimulants such as Angiotensin 1-7 and PDGF (Platelet Derived Growth Factor). Experimental results show that compared with a traditional serum-containing culture medium, the preparation provided by the invention can significantly improve the proliferation and migration of skin fibroblasts and the expression ability of collagen I. Therefore, the invention provides a cell culture scheme with definite components and excellent performance, and the cell culture scheme can be used for preparing medicines or products in the fields of skin repair, aging resistance and the like.

This invention belongs to the field of nanobiomedicine technology, specifically relating to a mitochondrial-targeting copper-based nanoparticle (Qu@Cu-SS31) and its application in the preparation of drugs for treating myocardial infarction. The nanoparticles consist of quercetin (Que) and copper ions (Cu²⁺). + The nanoparticles, consisting of a self-assembly of the mitochondrial-targeting peptide SS31, exhibit a uniform, near-spherical morphology with an average particle size of 17.2 ± 1.6 nm. These nanoparticles specifically target cardiomyocyte mitochondria, responsively releasing Cu²⁺. + Furthermore, Qu@Cu-SS31 restores cardiomyocyte copper homeostasis by downregulating the copper metabolism negative regulator COMMD1, the copper uptake protein CTR1, and the copper chaperone protein CCS. Based on this, Qu@Cu-SS31 exhibits remarkable SOD-like and CAT-like enzyme activities, efficiently scavenging various reactive oxygen species / reactive nitrogen species and significantly reducing oxidative stress. Simultaneously, it inhibits NLRP3 inflammasome activation and Caspase-1-mediated pyroptosis, regulates the Bcl-2 / Bax balance to inhibit apoptosis, and promotes vascular endothelial growth factor expression and angiogenesis.

The present application provides a kind of drug composition and its application for targeting neuroinflammation, the composition is composed of active ingredient and natural nanometer delivery carrier for encapsulating active ingredient, the active ingredient includes fisetin, pyrrole quinoline quinone, resveratrol, bauhinia A, apigenin, saffron, quercetin and hair monkey element;The natural nanometer delivery carrier is the plant source extracellular vesicle of surface modification brain targeting molecule.The composition of the present application realizes the intervention to vascular cognitive impairment by multi-component scientific compatibility, combined with two-stage brain targeting delivery design, solves the pain points of low blood-brain barrier penetration rate and poor bioavailability of natural active ingredient, has the effect of strong anti-inflammatory and significantly improving cognitive function, excellent safety, can be used for preparing the medicine for preventing or adjuvant therapy neuroinflammation related vascular cognitive impairment, has very high clinical conversion value.

This invention discloses a dihydroquercetin cocrystal, its preparation method, and its applications. The dihydroquercetin cocrystal is prepared by combining dihydroquercetin extracted from species such as Siberian larch with small molecule substances, including betaine, L-proline, benzamide, nicotinamide, or isonicotinine. The dihydroquercetin cocrystal of this invention exhibits high thermal stability and higher apparent solubility and dissolution rate compared to dihydroquercetin itself. This facilitates the full utilization of dihydroquercetin's various pharmacological effects, such as antioxidant, antitumor, anti-apoptotic, anti-inflammatory, cardiomyocyte protection, hypoglycemic, improvement of vascular endothelial cell function, antiplatelet aggregation, and influence on bone metabolism. Furthermore, the preparation method is simple, allowing for large-scale industrial production and offering significant economic benefits.

The invention relates to the technical field of skin mucosa repair, in particular to a pharmaceutical preparation for repairing skin mucosa injury. According to the preparation, kaempferol, quercetin, 3-p-coumaroyl-1, 4, 6 '-triacetyl cane sugar, 3-p-coumaroyl-2', 3 ', 6'-triacetyl cane sugar, (+)-clove resinol and vitamin B12 which are extracted from Nanking cherries are used as active ingredients, the problems that an existing skin mucous membrane injury repairing preparation is poor in curative effect, long in repairing period, prone to scar remaining and the like are solved, and the efficient, safe and high-quality skin mucous membrane injury repairing effect can be achieved.

The application belongs to the field of drug delivery and particularly relates to a composite porous stent, a composite porous drug delivery system and application. The composite porous stent comprises crosslinked oxidized hyaluronic acid, methacrylated xanthan gum, gelatin and sodium alginate; the composite porous drug delivery system is constructed by loading hyaluronic acid-coated quercetin and ginsenoside Rh2 nanoparticles on the composite porous stent, and can precisely and targetedly deliver the loaded traditional Chinese medicine components to a lesion site, so as to achieve the purpose of high-efficiency, safe and targeted treatment of breast cancer.

The invention relates to the technical field of preparation of drug-resistant drugs, and discloses a pharmaceutical composition for reducing drug resistance of liver cancer cells, which is prepared from the following components in parts by mass: 10 to 20 parts of oxymatrine, 5 to 15 parts of curcumin, 8 to 15 parts of ginsenoside Rg38, 3 to 8 parts of sorafenib, 5 to 12 parts of quercetin and 3 to 8 parts of oridonin. Different components in the pharmaceutical composition have a synergistic effect and jointly play a role in reducing the drug resistance of the liver cancer cells, so that the drug resistance of the liver cancer cells can be effectively reduced, and the safety is high.

The invention relates to a dihydroquercetin nano preparation and application thereof in treatment of pulmonary nodules, the dihydroquercetin nano preparation is in an irregular spherical shape, C, O, N and Ce elements coexist, the molar ratio of Ce < 4 + > to Ce < 3 + > is 1.57, and the dihydroquercetin nano preparation has enzyme-like activity of superoxide dismutase, catalase, peroxidase and glutathione and can realize enzyme cascade reaction; the preparation method of the dihydroquercetin nano preparation comprises the following steps: step 1, respectively dissolving Ce (NO3) 3.6 H2O and dihydroquercetin in a beaker filled with methanol; and 2, adding the two solutions into a beaker filled with a PEG 8000 methanol solution, starting an assembly process, and stirring at room temperature to obtain qualified CeDHQNCs, namely the dihydroquercetin nano preparation. The dihydroquercetin nano preparation shows excellent enzyme-like activity and high active oxygen scavenging ability, can inhibit pulmonary nodule growth and inflammation infiltration, and provides a new means for pulmonary nodule treatment.

The invention discloses radix astragali and radix ginseng palpitation relieving granules and a quality detection method thereof. The optimal forming process of the radix astragali and radix ginseng palpitation relieving granules is screened out through a large number of experiments. According to the invention, the optimal thin-layer chromatography development condition is screened out, and qualitative identification research is carried out on salvia miltiorrhiza, radix astragali preparata and ligusticum wallichii in the quality detection method of the radix astragali, radix astragali and radix astragali palpitation relieving granules. According to the present invention, the optimal fluidity composition, the gradient elution mode and other chromatographic conditions are screened through a large number of experiments, the fingerprint detection method with characteristics of high precision, good stability and high accuracy is established, and the contents of the effective components such as calycosin-7-glucoside, ferulic acid, dihydroquercetin, ammonium glycyrrhizinate, nardosinone and the like can be simultaneously determined; and a quality detection method is provided for ensuring the safety and the effectiveness.

The invention belongs to the technical field of biological medicines, and particularly relates to a preparation method and application of an anti-hepatic fibrosis nano-drug. The drug is a macrophage membrane coated dihydroquercetin loaded PLGA nanoparticle, PLGA-PEG is used as a nano-carrier to load an anti-hepatic fibrosis active component dihydroquercetin, and the outer layer is coated with a macrophage membrane to construct a bionic drug delivery system. Results of embodiments prove that the nano-drug provided by the invention can be selectively enriched in hepatic fibrosis focuses, can accurately release dihydroquercetin, cooperatively intervenes the hepatic fibrosis process through multiple ways such as oxidation resistance, inflammation resistance, hepatic stellate cell activation inhibition and immune microenvironment regulation, has small influence on normal hepatic tissues, and can be used for preparing a drug for treating hepatic fibrosis. Good clinical transformation potential is realized.

The invention relates to the technical field of biological medicines, in particular to a pharmaceutical composition with a synergistic anti-aging effect as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition specifically consists of dihydroquercetin and fisetin. According to the invention, two core anti-aging mechanisms of oxidation resistance and senescence cell removal are organically integrated for the first time, and multi-target collaborative intervention on the senescence process is realized. Compared with a single component, the composition not only can effectively repair oxidative damage, but also can selectively remove accumulated senescent cells, plays a role from the source and the result at the same time, overcomes the defects that a traditional single strategy is single in action path and limited in effect, and has a good application prospect. A more comprehensive and efficient new scheme is provided for delaying senescence and preventing and treating senescence-related diseases.

This invention relates to the field of biomedical technology and provides a method for preparing a multifunctional nanocomposite material and its applications. The material exhibits good biocompatibility and can be used as a safe agent for tumor treatment; its preparation is simple, green, and pollution-free; it expands the application of quercetin in anti-tumor immunotherapy; it possesses excellent fluorescence / computed tomography dual-mode imaging performance, which can be used to determine tumor boundaries and achieve precise treatment guidance; it has excellent photothermal and photothermally enhanced catalytic properties, possessing advantages such as minimally invasive and highly efficient treatment with few and controllable toxic side effects; it can effectively treat primary, metastatic, or recurrent head and neck squamous cell carcinoma by activating immunity; and by inducing tumor cells to release 2'3'-cGAMP and preventing its degradation, it efficiently activates the cGAS-STING signaling pathway, ultimately enhancing the anti-tumor immune effect.