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A method for the identification of dihydroquercetin in larch by capillary electrophoresis-mass spectrometry

A technology of dihydroquercetin and capillary electrophoresis, applied in the field of analysis and identification of dihydroquercetin, can solve the problems of high analysis cost, long detection time, and large sample usage, and achieve high experimental repeatability and sample usage The effect of small amount and high precision

Active Publication Date: 2019-03-12
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

[0007] Aiming at the shortcomings of the current dihydroquercetin identification methods, such as high analysis cost, large sample usage, and long detection time, the present invention provides a dihydroquercetin identification method using capillary electrophoresis-mass spectrometry, which can be highly efficient, Rapid identification of dihydroquercetin in larch

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  • A method for the identification of dihydroquercetin in larch by capillary electrophoresis-mass spectrometry
  • A method for the identification of dihydroquercetin in larch by capillary electrophoresis-mass spectrometry
  • A method for the identification of dihydroquercetin in larch by capillary electrophoresis-mass spectrometry

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specific Embodiment

[0029] (1) Using methanol as a solvent, prepare dihydroquercetin standard solutions at concentrations of 2000, 1000, 500, 250, and 125 μg / mL, respectively.

[0030] (2) Inject the sample in the capillary electrophoresis instrument, use 20mbar to inject the sample for 2s, and perform electrophoresis under the condition of 20kV. The ultraviolet wavelength is 214nm, the column temperature is 25°C, and the buffer salt is 20mmol ammonium acetate solution; / MS full scan, nitrogen flow rate 15psi, helium 7psi, gas temperature 300°C, separation voltage 3.0kV, sheath fluid using 50% methanol solution (containing 0.01% ammonium acetate), flow rate 100nL / min, pump flow rate 0.4L / min, the split ratio is 100:1, each concentration is loaded repeatedly 3 times, the peak area is integrated and calculated with Chemstation software, and the standard curve is drawn according to the obtained results. The results are shown in figure 1 , and measured the lowest detection limit of 15.6pmol.

[0031...

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Abstract

The invention provides a method for analyzing and identifying dihydroquercetin in larches by capillary electrophoresis-mass spectrometry (CE-MS). The method includes the steps: (1) preparing standardsolution of pure dihydroquercetin, injecting a sample into a capillary electrophoresis apparatus and performing analysis in a mass spectrometer to obtain a mass spectrum; (2) drawing a standard curveand calculating the lowest detection limit; (3) acquiring the mass spectrum of the sample by a method in the step (1) and calculating sample injection recovery rate; (4) identifying the dihydroquercetin in the larches according to sample chromatographic peak and characteristic daughter ion information; (5) quantitatively analyzing the dihydroquercetin in the larches according to the standard curve. The method has the advantages of simplicity in operation, time-saving property, high detection efficiency, good repeatability and the like. The needed sample is less in dosage, detection results areaccurate and reliable, and the method is an excellent method for qualitatively and quantitatively analyzing the dihydroquercetin in the larches.

Description

technical field [0001] The invention relates to an identification method for dihydroquercetin, in particular to an analysis and identification method for dihydroquercetin established by capillary electrophoresis-mass spectrometry (CE-MS). Background technique [0002] Dihydroquercetin (DHQ), also known as Taxifolin, Taxifolin, and Taxifolin, is a kind of dihydroflavonoids, and its structural formula is as follows. In nature, the content of dihydroquercetin is low, but the distribution is relatively wide, such as widely present in Pinaceae, Salicaceae, Rosaceae, Anacardiaceae, Lamiaceae and other plants, among which in the root neck of larch highest content. After larch was isolated, the content of dihydroquercetin decreased continuously with the increase of storage time. [0003] [0004] Dihydroquercetin was originally a kind of glucoside extracted by Japanese scholar Fukui, the molecular formula is C 15 h 12 o 7 , with a molecular weight of 304.25, is a colorless n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N27/62
CPCG01N27/4473G01N27/44743G01N27/44791G01N27/62
Inventor 盖颖王午豪代建容蒋湘宁
Owner BEIJING FORESTRY UNIVERSITY
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