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Method of purifying high content DHA, EPA from fish oil

A high-content, fish oil technology, applied in fatty acid production, fatty acid preparation/refining, fatty acid esterification, etc., can solve the problems of unsatisfactory separation effect of vacuum fractionation method, difficult mass production, complicated operation, etc., and improve people's health. level, elimination of side effects, and the effect of high product purity

Inactive Publication Date: 2005-10-19
天津市医药科学研究所
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But, the method in the above prior art can not easily obtain high-purity DPA, DHA product because of higher cost or complicated operation.
For example, the separation effect of the vacuum fractionation method is not ideal, and the product is easily overheated and oxidized; the cost of the molecular distillation equipment is relatively high, generally 20-30 kg per hour for the refining and separation of fish oil, and the cost is about 1.3 million yuan; the cost of the supercritical extraction method equipment The cost is greater; the urea inclusion method has strict restrictions on the experimental conditions, and the operation is cumbersome. Generally, under low temperature conditions (-30°C), the nitrate solution extraction method is not easy to carry out mass production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Materials and instruments, physical and chemical indicators and testing methods

[0018] 1.1 Materials and reagents Crude fish oil (provided by Zhoushan Fishery Company): absolute ethanol (AR Tianjin Reagent Factory II): silver nitrate (AR Tianjin Reagent Factory No. 1): concentrated sulfuric acid (AR Tianjin Reagent Factory II): n-hexane ( AR Beijing Chemical Factory)

[0019] 1.2. Experimental Instruments

[0020] Separating funnel: electric stirrer: magnetic stirrer: round bottom bottle: triangular flask: rotary thin film evaporator: water circulation pump: vacuum oil pump

[0021] 1.3. Product determination method DHA, EPA content determination: gas chromatography

[0022] 2. Experimental part Using domestic crude fish oil as raw material, first ethyl esterification, and then through Ag + The characteristics of forming complexes with unsaturated double bonds make DHA, EPA and Ag containing unsaturated double bonds in fish oil + The reaction generates a comple...

Embodiment 2

[0036] 1. Materials and instruments, physical and chemical indicators and testing methods

[0037] 1.1 Materials and reagents Crude fish oil (provided by Zhoushan Fishery Company): absolute ethanol (AR Tianjin Reagent Factory II): silver nitrate (AR Tianjin Reagent Factory No. 1): concentrated sulfuric acid (AR Tianjin Reagent Factory II): n-hexane ( AR Beijing Chemical Factory)

[0038] 1.2. Experimental Instruments

[0039] Separating funnel: electric stirrer: magnetic stirrer: round bottom bottle: triangular flask: rotary thin film evaporator: water circulation pump: vacuum oil pump

[0040] 1.3. Product determination method DHA, EPA content determination: gas chromatography

[0041] 2. Experimental part Using domestic crude fish oil as raw material, first ethyl esterification, and then through Ag + The characteristics of forming complexes with unsaturated double bonds make DHA, EPA and Ag containing unsaturated double bonds in fish oil + The reaction generates a comple...

Embodiment 3-5

[0058] From the above results, it can be concluded that at room temperature, AgNO 3 - Separation of AgNO from DHA and EPA by water method 3 The optimal concentration is 70%, the purity of the extraction is high, and the product is a colorless and transparent solution.

[0059] We have also confirmed through pharmacological experiments that at an appropriate dose, fish oil can not only significantly increase the level of serum high-density lipoprotein cholesterol (HDL-G), but also significantly reduce serum total cholesterol (TC) and triglyceride (TG). ) and low-density lipoprotein cholesterol (LDL-C) levels, indicating that fish oil has a significant effect of lowering blood fat in rats with experimental hyperlipidemia. The results also confirmed that high-content fish oil and American fish oil had basically the same effect on lowering fat in rats with experimental hyperlipidemia.

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Abstract

A process for extracting DHA and EPA from fish fat and purifying them features that the AgNO3 solution method is used to separate the ethylated product of fish fat and purify it. Its advantages are high purity (95%) for DHA and / or EPA, low toxic by-effect, and high stability of DHA and EPA.

Description

technical field [0001] The present invention relates to a method for obtaining fat, oil or fatty acid from fat, oil or fatty acid derived therefrom through chemical modification. More specifically, the present invention relates to a method for purifying high-content DHA and EPA from crude fish oil. Background technique [0002] It was discovered long ago that in patients with cardiovascular and cerebrovascular diseases, C in red blood cells 20 -C 22 The content of unsaturated fatty acids (Polyunsaturated fatty acids PUFAS) is significantly lower than that of normal people, and supplementing C 20 -C 22 PUFAS, after a few days, the patient’s membrane lipid composition changed significantly, PUFAS increased significantly, and blood lipids decreased accordingly. In the mid-1960s, Gunning et al. further affirmed that the ratio of PUFAS in the diet and the decrease in serum total cholesterol had a very significant regression relationship (r= 0.9), until 1978 when Dyerberg et a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11C1/08C11C3/04
Inventor 陶遵威解红武周运琴刘惠芝
Owner 天津市医药科学研究所