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Protein SM1 of hemopoietic stem cell surface

A hematopoietic stem cell and protein technology, applied in the field of protein and body, can solve the problem that CD34-enriched cells cannot form a hematopoietic stem cell population

Inactive Publication Date: 2000-08-02
干细胞治疗公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these primitive cells do display certain properties associated with hematopoietic stem cells, such as high proliferative capacity and the ability to differentiate into a variety of hematopoietic cell lineages, it is arguable that CD34-enriched cells do not constitute truly pure hematopoietic stem cell population

Method used

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  • Protein SM1 of hemopoietic stem cell surface
  • Protein SM1 of hemopoietic stem cell surface
  • Protein SM1 of hemopoietic stem cell surface

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Embodiment Construction

[0026] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0027] One protein (SM1) was found and subsequently purified to be substantially free of other proteins. It has a molecular weight of approximately 230 KDa as determined by immunoprecipitation and SDS-PAGE. SM1 is present on the surface of hematopoietic stem cells and primitive precursor cells in humans and mice, but not on other cell types including: FDC-P1 myeloid precursor cells, EL4 T-cells, WEHI-3 myelomonocytic cells, and 70Z / 3 pre-B lymphoid cells, or on the surface of differentiated hematopoietic cells absent from human cord blood or mouse bone marrow. anti-SM1 antibody

[0028] In one embodiment, the invention relates to anti-SM1 antibodies. In addition to using them to enrich hematopoietic stem cells, such antibodies may also provide research and diagnostic tools in the study of hematopoietic factors and the development of antibody-conjugated therapeutics. In addition, pharmaceutical compositio...

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Abstract

A protein designated 'SM1' has a molecular weight of about 230 kDa protein, as measured by immunoprecipitation and SDS-PAGE. SM1 proteins are present on the surface of human and mouse hematopoietic stem cells, respectively, and on primitive progenitor cells, but are absent from the surfaces of other cells, such as FDC-P1 myeloid progenitor cells, EL4 T-cells, WEHI-3 myelomonocytic cells, and 70Z / 3 pre-B lymphoid cells, or from differentiated hematopoietic cells of human cord blood or mouse bone marrow. Anti-SM1 antibody can be employed in making a preparation that is enriched for hematopoietic stem cells.

Description

Background of the invention [0001] The present invention relates to a protein referred to herein as SM1 which exists substantially in a purified form free of other proteins. With a molecular weight of approximately 230 KDa by immunoprecipitation and SDS-PAGE, SM1 is present on the surface of human and mouse hematopoietic stem cells and primitive precursor cells, but not on other cells, including: FDC-P1 myeloid Precursor cells, EL4 T-cells, WEHI-3 myelomonocytic cells, and 70 Z / 3 pre-B lymphoid cells, or differentiated hematopoietic stem cells that are also not present in human cord blood or mouse bone marrow surface. The invention still further relates to methods of generating a hematopoietic stem cell-enriched population using anti-SM1 antibodies. [0002] All circulating blood cells are formed from pluripotent stem cells through the process of hematopoiesis. Hematopoietic stem cells are undifferentiated cells capable of self-renewal and differentiation into committed pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/705C07K16/28G01N33/569G01N33/68
CPCG01N33/56972G01N33/6872C07K16/28C07K14/705
Inventor P·M·C·王S-W·钟X·韩H·陈
Owner 干细胞治疗公司
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