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Immunochromatographic test paper for detecting Yersinia pestis infection and preparing process thereof

An immunochromatographic test strip, Yersinia technology, applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems such as the immunocolloidal gold technology that has not yet been seen, and achieve the effect of strong specificity

Inactive Publication Date: 2006-07-19
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the use of immunocolloidal gold technology for the detection of Yersinia pestis infection

Method used

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  • Immunochromatographic test paper for detecting Yersinia pestis infection and preparing process thereof
  • Immunochromatographic test paper for detecting Yersinia pestis infection and preparing process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1. Preparation of immunochromatographic test paper for detection of Yersinia pestis infection

[0037] 1. Materials

[0038] Chloroauric acid (HAuCl 4 .4H 2 O), analytically pure, Shanghai Reagent No. 1 Factory;

[0039] Trisodium citrate (Na 3 C 6 H 5 O 7 .2H 2 O), analytically pure, Beijing Chemical Plant;

[0040] Potassium carbonate (K 2 CO 3 ), analytically pure, Beijing Chemical Plant;

[0041] Staphylococcus aureus protein A (SPA), purchased from Amersham Pharmacia Biotech;

[0042] Yersinia pestis strain, (introduced from Russia, the strain name is E.V, and is now preserved by the strain library of the Institute of Microbiology and Epidemiology of the Chinese People's Liberation Army, and the deposit number is 410049);

[0043] Plague positive serum national standard, produced by the National Institute of Biological Products;

[0044] Plague positive and negative sera from guinea pigs, chinchillas, rabbits and humans, provided by the Bruc...

Embodiment 2

[0056] Example 2. Detection comparison between two kits of plague colloidal gold method for rapid diagnosis of immunochromatographic test paper and indirect hemagglutination method

[0057] 1. Materials

[0058] Same as Example 1.

[0059] 2. Method

[0060] 2.1. Plague colloidal gold method for rapid diagnosis of clinical serum samples by immunochromatographic test strips

[0061] The serum sample to be tested was diluted 1:40 with normal saline, and 120 μl (about 3-4 drops) was added to the detection hole of the plague colloidal gold method rapid diagnostic kit. The results were observed after 5 minutes, and the observation was terminated after 20 minutes. Result report: 1 red (quality control) precipitation line appears, which is negative for Yersinia pestis serological diagnosis, that is, no Yersinia pestis infection; 2 red (quality control and control) precipitation lines appear, which are Yersinia pestis serological diagnosis is positive, that is, Yersinia pestis infe...

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Abstract

This invention discloses a rat pestis Yersinia inflection test paper and its process method. The test paper comprises the following: water absorption paper; nitric acid film tightly connected with the absorption paper; albumen A mark glue gold probes pads with gold staphylococcus on the nitric acid film; and specimen pad tight to the gold pad. The said nitric acid fiber film has the test line and control line, wherein the said test line is the rat pestis Yersinia solvable albumen amylose compound antigen and the said control line is the antibody combined with the gold staphylococcus albumen A.

Description

technical field [0001] The invention relates to a test paper for detecting Yersinia pestis infection, in particular to an immunochromatographic test paper for detecting Yersinia pestis infection, and a preparation method thereof. Background technique [0002] Plague (plague), also known as the Black Death, is a typical natural foci disease, usually occurs in rodents (such as rats, mice, chinchillas). It is a deadly and transmissible bacterial disease caused by the bacterium Yersinia pestis. [0003] At present, more than 20 countries in the world have reports of local outbreaks of plague every year. Although it has been more than a century since the discovery of plague, there are still many difficulties in the laboratory diagnosis of clinically suspected patients, especially there are not many methods suitable for rapid screening at the grassroots level. The lack of early diagnosis and treatment of the disease is a major cause of transmission and death among people in poor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/544G01N33/532
Inventor 端青朱虹檀华何君苏裕心
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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