Micro cuttage transplanting method for teakwood tissue cultivating seedlings

Abstract

The invention relates to a method for micro-cutting transplantation of teak tissue cultured seedlings, which is characterized in that tissue culture and cutting propagation are organically combined, and special treatment is performed on the tissue cultured seedlings to realize the cutting transplantation of the multiplied seedlings. The specific steps are as follows: 1. Seedlings: Proliferated seedlings are moved to the solar glass greenhouse when the height of the seedlings reaches 3cm indoors, and the seedlings are trained. 2. Pre-treatment: Take the seedlings that have been trained from the culture bottle, wash the medium, cut off the base of the seedlings and bring healing 3. Micro-cutting: learn from the cutting technology, dip the pre-treated seedlings in special rooting powder on the base, and then transplant them directly into the seedling bags. 4. Post-planting management: manage the transplanted seedlings around the clock. The present invention realizes the leap from research to production. With the application of the present invention, any tissue culture factory can transplant teak tissue culture seedlings into qualified afforestation bag seedlings, and the production nursery field can also successfully transplant the seedlings that have completed hardening. Reach more than 95%, save cost 0.1 yuan / plant.

Description

technical field

[0001] The invention relates to a method for micro-cutting transplantation of teak tissue culture seedlings, and belongs to the technical field of cultivation and transplantation of tropical forest trees, especially teak. Background technique

[0002] Teak (Tectona grandis L.F.) is native to India, Myanmar, Thailand, Laos and other countries. Teak wood has excellent wood properties and is one of the most famous and precious materials in the world. my country began to introduce teak in the 1960s, and has planted more than 3,000 hectares in Yunnan, Hainan, Guangxi and other places so far. However, due to its late maturity (more than 20 years) and late flowering and fruiting (more than 10 years), production and development are limited. Impact. The Tropical Forestry Research Institute of the Chinese Academy of Forestry has selected a large number of fast-growing and resistant excellent provenances and clones through more than 30 years of introduction and breeding...

Examples

Embodiment 1

[0033] Embodiment 1: the applicant adopts the present invention to produce 100,000 strains of teak tissue culture bag seedlings, with 100 bottles (7 strains / bottle) multiplication seedlings as base, by going out bottle qualified seedling 97%, nursery transplant survival rate 95% calculates, only The task of raising seedlings was completed in June. Specific steps are as follows:

[0034] 1) training seedlings: the multiplication seedlings are moved to the solar glass greenhouse when the height of the indoor cultivation reaches 3cm, and the seedling training is carried out. The temperature of the greenhouse is controlled at 30°C, and the light intensity is 7500lx. After 23 days of training, the seedlings are trained; The morphological characteristics of the seedlings are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0035] 2) Pre-treatment: Take out the seedlings that have complet...

Embodiment 2

[0041] Embodiment 2: concrete steps are as follows:

[0042] 1) training seedlings: the multiplication seedlings are moved to the solar glass greenhouse when the height of the indoor cultivation reaches 3cm, and the seedling training is carried out. The temperature of the greenhouse is controlled at 25° C., and the light intensity is 10000 lx. After training the seedlings for 25 days; after training the seedlings The morphological characteristics of the growth are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0043] 2) step to 4) step with embodiment 1.

Embodiment 3

[0044] Embodiment 3: concrete steps are as follows:

[0045] 1) training seedlings: the proliferating seedlings are moved to the sunlight glass greenhouse when the seedling height reaches 3cm indoors, and the seedling training is carried out. The temperature of the greenhouse is controlled at 35° C., and the light intensity is 4000 lx. After training the seedlings for 35 days; after training the seedlings The morphological characteristics of the seedlings are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0046] 2) step to 4) step with embodiment 1.

[0047] The active ingredients of the patented rooting powder used in Examples 1 to 3 of the present invention are indole butyric acid IBA, indole acetic acid IAA, and alpha naphthalene acetic acid NAA, and the concentration is 200-300PPm; Put it in a fresh-keeping box and put it in a refrigerator at 3-5°C for 2-3 months.

[0048] In ...

technical field

[0001] The invention relates to a method for micro-cutting transplantation of teak tissue culture seedlings, and belongs to the technical field of cultivation and transplantation of tropical forest trees, especially teak. Background technique

[0002] Teak (Tectona grandis L.F.) is native to India, Myanmar, Thailand, Laos and other countries. Teak wood has excellent wood properties and is one of the most famous and precious materials in the world. my country began to introduce teak in the 1960s, and has planted more than 3,000 hectares in Yunnan, Hainan, Guangxi and other places so far. However, due to its late maturity (more than 20 years) and late flowering and fruiting (more than 10 years), production and development are limited. Impact. The Tropical Forestry Research Institute of the Chinese Academy of Forestry has selected a large number of fast-growing and resistant excellent provenances and clones through more than 30 years of introduction and breeding...

Examples

Embodiment 1

[0033] Embodiment 1: the applicant adopts the present invention to produce 100,000 strains of teak tissue culture bag seedlings, with 100 bottles (7 strains / bottle) multiplication seedlings as base, by going out bottle qualified seedling 97%, nursery transplant survival rate 95% calculates, only The task of raising seedlings was completed in June. Specific steps are as follows:

[0034] 1) training seedlings: the multiplication seedlings are moved to the solar glass greenhouse when the height of the indoor cultivation reaches 3cm, and the seedling training is carried out. The temperature of the greenhouse is controlled at 30°C, and the light intensity is 7500lx. After 23 days of training, the seedlings are trained; The morphological characteristics of the seedlings are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0035] 2) Pre-treatment: Take out the seedlings that have complet...

Embodiment 2

[0041] Embodiment 2: concrete steps are as follows:

[0042] 1) training seedlings: the multiplication seedlings are moved to the solar glass greenhouse when the height of the indoor cultivation reaches 3cm, and the seedling training is carried out. The temperature of the greenhouse is controlled at 25° C., and the light intensity is 10000 lx. After training the seedlings for 25 days; after training the seedlings The morphological characteristics of the growth are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0043] 2) step to 4) step with embodiment 1.

Embodiment 3

[0044] Embodiment 3: concrete steps are as follows:

[0045] 1) training seedlings: the proliferating seedlings are moved to the sunlight glass greenhouse when the seedling height reaches 3cm indoors, and the seedling training is carried out. The temperature of the greenhouse is controlled at 35° C., and the light intensity is 4000 lx. After training the seedlings for 35 days; after training the seedlings The morphological characteristics of the seedlings are: the leaves of the seedlings are dark green, the leaves are wide, the stems of the seedlings are dark green, and they are initially lignified;

[0046] 2) step to 4) step with embodiment 1.

[0047] The active ingredients of the patented rooting powder used in Examples 1 to 3 of the present invention are indole butyric acid IBA, indole acetic acid IAA, and alpha naphthalene acetic acid NAA, and the concentration is 200-300PPm; Put it in a fresh-keeping box and put it in a refrigerator at 3-5°C for 2-3 months.

[0048] In ...