Protein-chip for prenated diagnosis and its preparing process

Abstract

A protein chip for prenatal diagnosis features that the protein or polypeptide array is sequentially arranged on solid substrate for testing, recognizing, discriminating and diagnosing the protein. It is prepared by loading the dot matrix of the antigens of Toxoplasma, rubella, cytomegalovirus and herpes simplex viruses A and B, positive reference and negative reference in a same cavity on biochip. Its advantages are one-step reaction for different results, high speed and sensitivity, and high stability and reliability.

Description

technical field [0001] The invention relates to a protein chip for prenatal diagnosis and a manufacturing method, relating to biochip technology. A protein chip is an array of proteins or polypeptides arranged in an orderly manner on a solid substrate to achieve parallel detection, identification, identification and diagnosis of proteins. It can be applied to biology, medicine and related fields. Background technique [0002] Toxoplasma, Rubella, Cytomegalovirus (CMV), Herpes Simplex Virus (HSV, type 1 and / or type 2) are five important indicators for prenatal examination of pregnant women , referred to as ToRCH. ToRCH is a group of widely spread pathogenic microorganisms. Due to endocrine changes and decreased immunity, pregnant women are prone to primary infection and recurrent infection, and can be transmitted to the fetus or newborn through the placenta, birth canal or breast milk. Fetal infection can lead to miscarriage, premature birth, stillbirth or fetal growth ret...

AI Technical Summary

Problems solved by technology

[0004] There are currently two types of ToRCH ELISA kits, one is item-by-item inspection, that is, 10 kits are required to detect all IgM and IgM, and each reaction can only detect one indicator, which is slow and inefficient. ;Another kit mixes five IgG or IgM indicators together. As long as the final color comparison exceeds the negative control, it is judged as an infection, but it is impossible to determine which indicator is abnormal. If a diagnosis is required, it must be re-tested item by item

Therefore, the ELISA method for ToRCH diagnosis is time-consuming and laborious. In addition, its sensitivity is also limited. The substrates used in the experiment are also highly toxic chemicals, which pose a threat to the experimenters.

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