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Notoginseng cell culture method by adding jasmone (acid) compound in culture medium

A technology of jasmonic acid and Panax notoginseng cells, which can be applied to plant cells, etc., and can solve the problems such as the inability to regulate the active ingredients of ginsenosides

Inactive Publication Date: 2002-03-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to disclose a method for culturing the Panax notoginseng cells that regulate the active ingredients of ginsenosides by adding jasmone (acid) compounds in the Panax notoginseng cell fermentation medium. At the same time, the technology of increasing the content of total ginsenosides can overcome the defect that the active ingredients of ginsenosides cannot be adjusted in the existing technology, and lay the foundation for the industrialized economic production of each monomer component of ginsenosides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The experiment used Panax notoginseng (Burk.) F.H.Chen cells induced by the roots of perennial Panax notoginseng plants. MS medium was used as control culture, and 2 mg / L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.7 mg / L 6-furfurylaminopurine (KT) and 30 / L sucrose were additionally added. The regulation of monomeric saponins adopts control medium to add dihydromethyl jasmonate (with the structure of (2) formula, wherein R=CH 3 ). Inoculate 2g of wet cells obtained by vacuum filtration into a 250ml Erlenmeyer shaker flask filled with 50ml of fresh medium, culture in the dark at 25°C, and weigh the cells after drying at 40°C. In addition, stem cells were extracted and processed according to the method of Ren and Chen (Ren and Chen, 1999), and the content of monomeric saponins was analyzed by high performance liquid chromatography. The results are shown in the table below (cultured for 12 days). Dihydromethyl jasmine dry weight saponin content (mg / 100ug dry weight)

[0...

Embodiment 2

[0020] The regulation of monomeric saponins adopts control medium to supplement methyl jasmonate (with the structure of (1) formula, wherein R=CH3 ), other conditions are the same as example 1. The results are shown in the table below (cultured for 15 days). Methyl Jasmone Saponin Content(mg / 100mg DW)

[0021] Dry weight (g / L) Rb: Rg ester addition amount Rg1 Re Rb1 Rd 0 7.97±0.01 0.32±0.02 0.43±0.03 0.37±0.02 0 0.49±0.06 20μM 7.93±0.10 0.83±0.15 0.78±0.12 1.67±0.23 0. 0.01 1.12±0.04 100μM 7.71±0.27 0.86±0.04 0.79±0.02 2.53±0.12 0.17±0.02 1.64±0.03 200μM 8.14±0.01 0.82±0.00 0.80±0.01 3.15±0.08 0.20±0.01 2.06±0.07 500μM 8.09±0.44 0.46±0.05 0.45 ±0.05 2.03±0.17 0.10±0.01 2.49±0.06 In the table: Rb: Rg=(Rb 1 +Rd) / (Rg 1 +Re).

[0022] As can be seen from the above results, compared with the control (without adding methyl jasmonate), after adding 200 μM methyl jasmonate, the content of saponins was greatly improved, and the ratio of each monomer also c...

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Abstract

The present invention discloses a notoginseng cell culture method, and is characterized by adding jasmone (jasmonic acid) compound in its culture medium, not only can promote synthesis of total saponin, but also, it is more important, can change the ratio between all monomer saponins so as to successfully implement regulation and control of monomer saponin synthesis, and lay foundation for large-scale production of directional monomer saponin.

Description

technical field [0001] The invention relates to a method for culturing Panax notoginseng cells, in particular to a method for cultivating a medium in which substances capable of regulating ginsenoside components are added. Background technique [0002] Panax notoginseng (Burk.) F.H.Chen, also known as Tianqi, Panax notoginseng, Panax notoginseng, etc., is a plant of the genus Panax of Araliaceae, mainly distributed in Yunnan, Guangxi and other places in southwest my country. "Compendium of Materia Medica" contains its medicinal effects, such as promoting blood circulation and removing blood stasis, calming and relieving pain, stopping bleeding and nourishing blood, and nourishing and strengthening. Modern medical research has revealed that triterpenoid ginsenosides are one of its main biologically active components. Ginsenosides are not a single component, they are of many types, and can be divided into dammarane-type tetracyclic triterpene saponins and oleanane-type pentacy...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 钟建江王威
Owner EAST CHINA UNIV OF SCI & TECH
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