The present application relates to the technical field of biological
medicine preparation, and particularly relates to a recombinant
fusion protein of
semaglutide precursor
peptide,
polynucleotide,
recombinant expression plasmid, engineered host
cell and a method for preparing a target polypeptide. The recombinant
fusion protein of the present application is
fusion peptide-target polypeptide-(connecting
peptide-target polypeptide)n from N terminal to C terminal, wherein the
isoelectric point of the recombinant
fusion protein is 4.0-5.0, the average hydrophilicity of the recombinant fusion
protein is-0.500--0.905, the
fusion peptide is SEQ ID No. 4, the connecting
peptide is SEQ ID No. 11, and n is a positive integer not less than 1. The recombinant fusion
protein of the present application is expressed in the form of inclusion body, which simplifies the subsequent
processing technology, improves the expression amount of the recombinant fusion
protein and the yield of the target polypeptide, and has high
enzyme cutting efficiency, thereby reducing the cost from multiple dimensions.