Simple and fast light microscope detection technology for blood and lymph of live shrimp and crab

A detection technology, a hemolymph technology, is applied in the field of simple and rapid detection technology of live shrimp and crab hemolymph, which can solve the problems of complex Giemsa staining, difficulty in promotion and use, and time-consuming, so as to facilitate real-time physiological detection and reduce blood loss. Contamination chance, effect of easy blood collection volume

Inactive Publication Date: 2002-05-22
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0028] The hemolymph of shrimps and crabs is thicker and more likely to coagulate than the blood of higher animals. The dyeing methods of the above higher animal blood are often ineffective; the conventional Giemsa staining method is complex, cumbersome, tim

Method used

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  • Simple and fast light microscope detection technology for blood and lymph of live shrimp and crab
  • Simple and fast light microscope detection technology for blood and lymph of live shrimp and crab
  • Simple and fast light microscope detection technology for blood and lymph of live shrimp and crab

Examples

Experimental program
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Embodiment 1

[0046] Embodiment 1, with reference to figure 1 , figure 2 : Clean the shrimp and crab in vitro, and use 70% alcohol cotton ball to wipe the joint of the abdominal segment of the shrimp or the base of 3-4 pairs of legs of the crab twice for disinfection. Use a 1mL syringe to draw 0.2-0.3mL of alcohol in the alcohol cotton ball, and draw back and forth in the syringe to disinfect the inside of the syringe. Push out the alcohol in the tube, wipe the needle with an alcohol cotton ball, pierce the shrimp and crab at the sterilized part of the shrimp and crab by 7-10 mm, and draw the required amount of blood.

[0047] Preparation and staining of blood smear

[0048] Take about 0.05mL of hemolymph, drop it on a clean glass slide, and immediately add an equal amount of 4% formaldehyde fixative to the drop

[0049] After the drops are dried naturally, put them into Giemsa staining solution and stain for 15-20 minutes

[0050] After rinsing with water, the stained slides can be us...

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Abstract

The prevent invention relates to a simple and quick detectino technique of shrimp-crab intravital blood lymph light microscope, including shrimp and crab living body blood-taking method: cleaning shrimp and crab body, disinfecting by using alcohol, using injector to needle into 7-10 mm of shrimp or crab body to draw blood; blood film preparation and staining method, using 0.05 ml of blood-lymph fluid, dropping on glass slide, adding 4% formaldehyde fixing fluid, drying, staining for 15-20 min. in Giemsa staining solution, washing film with water and making observation by using light microscope, covering with glass plate and making observation by using oil microscope. Said invention adopts meta-acid water flushing scheme to can quickly detect pathogen RLO (Rickettsia-like organism), therefore it possesses high practical value for culturing shrimp and crab.

Description

technical field [0001] The invention relates to a detection technology in aquaculture, in particular to a simple and rapid detection technology of live shrimp and crab hemolymph light microscopy. Background technique [0002] In the aquaculture industry, it is necessary to frequently check the health status of shrimp and crabs in order to prevent and control diseases, or use the test results as quality indicators for commercial shrimps and crabs. Among them, the light microscope detection technology of living shrimp and crab blood lymph is the most commonly used detection technology, including blood collection technology and smear staining technology. The existing blood collection technology is to drill a small hole in the heart area of ​​the carapace of shrimps and crabs, and insert the pericardial sinus with a straw to draw hemolymph. Another way of blood collection is to take blood from the feet. The former method of blood collection is lethal destruction; the latter is...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N21/00G01N33/48G01N33/50
Inventor 王文
Owner NANJING NORMAL UNIVERSITY
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