A probiotics microcapsule for feeding is characterized in that the probiotics in the probiotics microcapsule is composed of bifidobacterium animalis, enterococcus faecium, and lactobacillus casei. Theprobiotics microcapsule also comprises a protective agent, which comprises glycerin, skim milk powder, ascorbic acid, lycium barbarum polysaccharide, xylooligosaccharide, tryptophan, and sterile water. According to the preparation method, lycium barbarum polysaccharide, tryptophan, and xylooligosaccharide are combined, then the composition is combined with common protective agent components, through the cooperation among the components, the high activity of live bacteria in the probiotics microcapsule can be maintained, the live bacteria will not be destroyed or damaged by chemical componentsand low temperature during the processing process, and at the same time, after preservation, the live bacteria still have high proliferation activity.