Probiotics microcapsule for feeding and preparation method and applications thereof

A technology of probiotics and microcapsules, which is applied in the field of feed probiotic microcapsules and its preparation, can solve the problems of difficulty in meeting the rapid development needs of the breeding industry and the lack of product types, and achieve low destruction rate, high pass rate and high proliferation active effect

Inactive Publication Date: 2018-10-19
九江牧威利元科技中心(普通合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, based on the above existing literature reports and patent literature publications, it can be known that the development of feed probiotic microcapsules is still in its infancy, and there are few types of industrialized products, which are difficult to meet the rapid development needs of the breeding industry.

Method used

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  • Probiotics microcapsule for feeding and preparation method and applications thereof
  • Probiotics microcapsule for feeding and preparation method and applications thereof
  • Probiotics microcapsule for feeding and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: a kind of preparation method of probiotic microcapsules for feeding comprises the steps:

[0030] Step 1: respectively activate and cultivate Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei to obtain bacterial fluids of Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei, then centrifuge to obtain sludge sediments, wash with PBS The sludge was precipitated twice, and after centrifugation, the sludge of Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei were obtained;

[0031] Step 2: Weigh the bifidobacterium animal sludge, Enterococcus faecium sludge and Lactobacillus casei sludge prepared in step 1 according to the mass ratio of 1:2:1, mix them evenly to obtain a mixed sludge, and mix the mixed sludge with the protective Suspend the mixed bacteria slime into the protective agent according to the mass volume ratio of 1:5, and shake and mix evenly to obtain the mixed bacteria liquid;

[0032] S...

Embodiment 2

[0039] Embodiment 2: a kind of preparation method of feeding probiotic microcapsules, comprises the steps:

[0040] Step 1: respectively activate and cultivate Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei to obtain bacterial fluids of Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei, then centrifuge to obtain sludge sediments, wash with PBS The sludge was precipitated 3 times, and after centrifugation, the sludge of Bifidobacterium animalis, Enterococcus faecium and Lactobacillus casei were obtained;

[0041] Step 2: Weigh the bifidobacterium animal sludge, Enterococcus faecium sludge and Lactobacillus casei sludge prepared in step 1 according to the mass ratio of 1:2:1, mix them evenly to obtain a mixed sludge, and mix the mixed sludge with the protective Suspend the mixed bacteria slime into the protective agent according to the mass volume ratio of 1:5, and shake and mix evenly to obtain the mixed bacteria liquid;

[0042] St...

Embodiment 3

[0049] Embodiment 3: the setting of comparative embodiment

[0050] Since the key to the relevant effects of probiotic preparations lies in the live bacteria content of probiotics, and during processing, the activity of probiotics is easily affected by the external environment, resulting in the death of probiotics. Therefore, it is necessary to ensure the activity of probiotics in the production process It is extremely important. Therefore, during the research and development process, the inventor focused on the research on the relevant components of the protective agent, and based on a large number of experiments, screened and obtained the best protective agent of the present invention. The following records the preferred protective agent components in the research and development process of the present invention. Although its protective effect is slightly worse than that of the present invention, it also gave the inventors a lot of inspiration. It is discussed together here, ...

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Abstract

A probiotics microcapsule for feeding is characterized in that the probiotics in the probiotics microcapsule is composed of bifidobacterium animalis, enterococcus faecium, and lactobacillus casei. Theprobiotics microcapsule also comprises a protective agent, which comprises glycerin, skim milk powder, ascorbic acid, lycium barbarum polysaccharide, xylooligosaccharide, tryptophan, and sterile water. According to the preparation method, lycium barbarum polysaccharide, tryptophan, and xylooligosaccharide are combined, then the composition is combined with common protective agent components, through the cooperation among the components, the high activity of live bacteria in the probiotics microcapsule can be maintained, the live bacteria will not be destroyed or damaged by chemical componentsand low temperature during the processing process, and at the same time, after preservation, the live bacteria still have high proliferation activity.

Description

Technical field: [0001] The invention belongs to the cross field between the field of feed and the field of biotechnology, and in particular relates to a probiotic microcapsule for feeding and a preparation method and application thereof. Background technique: [0002] With the continuous improvement of people's quality of life, the development of animal husbandry has gradually improved, and the safety of livestock and poultry products has also received widespread attention. Due to the large and unreasonable use of antibiotics, chemical drugs, and hormones in the production of livestock and poultry in my country, the safe production of livestock and poultry products has been seriously damaged. Especially for feed antibiotics, the application of feed antibiotics has a history of nearly 70 years, and more than 60 kinds of antibiotics have been used in animal husbandry successively, playing an important role in the prevention and control of animal diseases, improving feed utili...

Claims

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Application Information

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IPC IPC(8): A23K10/18
CPCA23K10/18
Inventor 张磊曾和华陈瑞
Owner 九江牧威利元科技中心(普通合伙)
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