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Preparation method and application of keratitis animal model

An animal model, keratitis technology, applied in the field of animal models, can solve the problems of many complications and other factors, complicated surgery, etc., and achieve the effect of small surgical trauma, simple nursing and short operation time

Active Publication Date: 2019-04-05
成都合拓创展生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To improve the problem of complex surgery and many other complications caused by the ocular branch injury model of the mouse trigeminal nerve

Method used

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  • Preparation method and application of keratitis animal model
  • Preparation method and application of keratitis animal model
  • Preparation method and application of keratitis animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The embodiment of the present application provides a method for preparing an animal model of keratitis.

[0074] 1. Exposure of the ophthalmic branch of the trigeminal nerve

[0075] a, anesthesia: weigh the quality of the male cyanotic blue rabbit to be 2.6kg, give the rabbit intravenous injection of 5.2mL of 1% pentobarbital sodium solution;

[0076] b. Opening: press the zygomatic fossa at the corner of the rabbit’s eye to slightly protrude the eyeball to expose the rectus oculi muscle, and use ophthalmic scissors to pull the fascia at the fornix between the lateral rectus muscle and the inferior rectus muscle on the lower edge of the rabbit’s eyeball to form an opening;

[0077] c. Remove obstacles: Use ophthalmic forceps to bluntly separate the fascia between the lateral rectus muscle and the inferior rectus muscle to make the lacrimal gland visible, and gently pull the lacrimal gland to the outside of the rabbit’s eyeball to make the inner canthal venous plexus in...

Embodiment 2

[0084] The embodiment of the present application provides a method for preparing an animal model of keratitis.

[0085] 1. Exposure of the ophthalmic branch of the trigeminal nerve

[0086] a, anesthesia: weigh the quality of the male cyanotic blue rabbit to be 2.4kg, inject 4.3mL of pentobarbital sodium solution with a mass fraction of 0.12% to the rabbit;

[0087] b. Opening: press the zygomatic fossa at the corner of the rabbit’s eye to slightly protrude the eyeball to expose the rectus oculi muscle, and use micro-scissors to pull the fascia at the fornix between the lateral rectus muscle and the inferior rectus muscle on the lower edge of the rabbit’s eyeball to form an opening;

[0088] c. Remove obstacles: Use micro-tweezers to bluntly separate the fascia between the lateral rectus muscle and the inferior rectus muscle to make the lacrimal gland visible, and gently pull the lacrimal gland to the outside of the rabbit’s eyeball to make the inner canthal venous plexus insi...

Embodiment 3

[0095] The embodiment of the present application provides a method for preparing an animal model of keratitis.

[0096] 1. Exposure of the ophthalmic branch of the trigeminal nerve

[0097] a, anesthesia: weigh the quality of the male cyanotic blue rabbit to be 2.7kg, inject 5.9mL of pentobarbital sodium solution with a mass fraction of 0.08% to the rabbit;

[0098] b. Opening: press the zygomatic fossa at the corner of the rabbit’s eye to slightly protrude the eyeball to expose the rectus oculi muscle, and use micro-scissors to pull the fascia at the fornix between the lateral rectus muscle and the inferior rectus muscle on the lower edge of the rabbit’s eyeball to form an opening;

[0099] c. Remove obstacles: Use micro-tweezers to bluntly separate the fascia between the lateral rectus muscle and the inferior rectus muscle to make the lacrimal gland visible, and gently pull the lacrimal gland to the outside of the rabbit’s eyeball to make the inner canthal venous plexus insi...

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Abstract

The invention relates to a preparation method and an application of a keratitis animal model and belongs to the technical field of animal models. The preparation method of the keratitis animal model comprises the following steps: forming an opening by pulling fascia between lateral rectus and inferior rectus on an inferior margin of a rabbit eye; clearing away or pulling barriers, and then keepingtrigeminal eye branch visible; separating the fascia surrounding the trigeminal eye branch, thereby exposing the trigeminal eye branch; transferring a NaOH solution into the exposed trigeminal eye branch. According to the invention, the opening is formed by pulling fascia between lateral rectus and inferior rectus on the inferior margin of the rabbit eye, so that the problems of large injury andmore complications of craniotomy can be avoided; operation time is short; suture is not required after operation; wound healing time is short; wound infection probability is small; the model has injury consistency. The invention also relates to the application of the keratitis animal model acquired according to the preparation method of the keratitis animal model in screening drugs for treating neurotrophic keratitis.

Description

technical field [0001] The present application relates to the technical field of animal models, and in particular to a preparation method and application of an animal model of keratitis. Background technique [0002] The distribution of the ophthalmic branch of the trigeminal nerve is quite different between humans and other animals (such as rats, monkeys, and dogs). There are many clinical diseases caused by damage to the ophthalmic branch of the trigeminal nerve, and neurotrophic keratitis is one of them. [0003] The ophthalmic branch of trigeminal nerve injury model is commonly made in mice, and the ophthalmic branch of trigeminal nerve is exposed by craniotomy, and the trigeminal nerve is injured by electric shock. This method involves craniotomy, and damages the cranial brain, which causes great damage to the animal's brain, and has many complications (such as changes in intracranial pressure), sutures are required after the operation, the wound healing time is long, t...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61K49/00
CPCA61K33/00A61K49/0008
Inventor 孙效容陈玥苏丹程勇
Owner 成都合拓创展生物科技有限公司
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