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Biochip compatible with 96 pore plate operating system

A biochip and operating system technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc., can solve the problems of time consumption, errors, expensive chips and operating systems, liberate manpower, improve Accuracy and stability, the effect of convenient marketing

Inactive Publication Date: 2003-01-15
GENETECH BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, although the above-mentioned biochip has greatly improved the efficiency compared with a single operation, it still requires a large number of repeated operations from sample preparation to sample addition, reaction, and cleaning, which not only consumes time, but also causes errors caused by human factors.
Although the Affymetrics company in the United States has developed a reaction workstation, it is limited to the gene chip developed by the company. This chip can only measure one sample. The preparation of the sample also needs to be completely manual, and the chip and operating system are expensive.

Method used

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  • Biochip compatible with 96 pore plate operating system
  • Biochip compatible with 96 pore plate operating system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] as attached figure 1 And attached figure 2 , the biochip compatible with the 96-well microplate operating system of the present invention is composed of a solid substrate 3 and a frame structure 1 with holes, and the solid substrate is activated by aldylation or amination, biotin, streptavidin, etc. Group treatment, which can be combined with a glass slide of biomolecules or biological tissue blocks; the frame structure with holes is consistent with the length, width, height, hole spacing and the distance from the center of the hole to the edge of the 96-well plate. The solid base and the perforated frame together form small pores 2 that are separated from each other but do not leak.

[0017] In this embodiment, the solid substrate is a glass sheet measuring 12 cm x 8 cm x 0.096 cm. The frame with holes is made of polystyrene, the shape is the same as that of a 96-well plate, the holes are 1 cm deep, there are no bottoms, and the number of small holes is 12×8, a tota...

Embodiment 2

[0020] The components are as in the first embodiment, the frame with holes is designed as a square hole, and the side length of the hole is 0.9 cm.

Embodiment 3

[0022] The solid substrate consists of 4 standard glass slides, each 7.5 cm x 2.5 cm x 0.096 cm.

[0023] The frame with holes is composed of a support part and a grid part. The support part is made of polystyrene and has the same length, width and height as a 96-well plate. The bracket of the solid base, the grid part is made of polystyrene or rubber, the size is consistent with the solid base, with round holes or square holes, with glue on one side, and can be glued to the solid base. After gluing, the hole spacing and hole The distance from the center to the edge of the support is consistent with that of a 96-well plate.

[0024] The production and use methods of this embodiment are as follows: first, the grid part is glued to the solid substrate, and placed on the corresponding part of the support part of the frame support part with holes. After sampling, it can be directly placed in the sample adding, reacting, washing After the operation is completed, the glued grid par...

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Abstract

A biochip compatible with the automatic operating system of 96-hole enzyme-linked board is composed of solid substrate and perforated frame structure. Said solid substrate is a glass plate, which has been aldehydized or ammonified and treated by biotin and chain avidin for binding biologic moleculae or biologic tissue blocks. Said perforated frame has the sizes same as those of 96-hole enzyme-linked board.

Description

technical field [0001] The biological chip compatible with the operating system of the 96-well microplate plate of the present invention relates to a biological chip capable of adding samples, reacting, cleaning and the like in the operating system of the 96-well plate. A biochip is a device that arranges biological samples in an orderly manner on a solid substrate to achieve parallel detection, identification, identification, and diagnosis of biomolecules or tissues. It can be applied to biology, medicine and related fields. Background technique [0002] The research and development of biochips has become a hot topic in biology and medical research at home and abroad. How to achieve high integration, parallelization, automation, and miniaturization to complete biological reactions is a particularly concerned issue in this topic. [0003] At present, the biochips that have been developed and applied more are mainly for spotting and processing directly on the solid support, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/25G01N33/535
Inventor 张涛李宾彭永济任一萍
Owner GENETECH BIOTECH SHANGHAI
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