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Fluorescence diagnostic reagent for gastric cancer

A diagnostic reagent and fluorescence technology, applied in biological testing, material inspection products, etc., can solve problems such as missed opportunities for early detection of gastric cancer, damage, etc., to achieve the effect of improving the speed of diagnosis, simplifying the diagnosis procedure, and reducing pain.

Inactive Publication Date: 2003-09-17
李莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a common disease, gastric cancer seriously endangers people's health and life safety. At present, in the process of diagnosis and treatment of gastric cancer, it is necessary to use gastroscopy and physiological slices to diagnose gastric cancer. Some patients need to undergo physiological sectioning to diagnose gastric cancer. During the sectioning process, the gastric tissue of the patient will inevitably be damaged to varying degrees. This is an unnecessary pain for ordinary patients with gastric diseases. Can only observe the surface of the stomach so may overlook some less obvious differences and miss the opportunity for early detection of gastric cancer

Method used

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  • Fluorescence diagnostic reagent for gastric cancer
  • Fluorescence diagnostic reagent for gastric cancer
  • Fluorescence diagnostic reagent for gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment one: the phosphoric acid of 0.1 gram, the disodium hydrogen phosphate of 19 grams, the boric acid of 1 gram, sodium tetraborate of 2 grams, 0.3 gram of tartaric acid, 0.1 gram of fluorescamine, 0.05 gram of anilinonaphthalene sulfonate, dipotassium amine Mix 0.01 g of naphthalenesulfonyl chloride and 0.2 g of sodium fluorescein.

Embodiment 2

[0017] Embodiment two: 1 gram of phosphoric acid, 0.1 gram of disodium hydrogen phosphate, 4 grams of boric acid, 30 grams of sodium tetraborate, 2.5 gram of tartaric acid, 0.5 gram of fluorescamine, 0.1 gram of anilinonaphthalene sulfonate, 0.3 gram of di Mix potassium aminonaphthalenesulfonyl chloride and 0.8 g sodium fluorescein.

Embodiment 3

[0018] Embodiment three: 5 grams of phosphoric acid, 10 grams of disodium hydrogen phosphate, 2 grams of boric acid, 10 grams of sodium tetraborate, 1 gram of tartaric acid, 0.3 gram of fluorescamine, 0.08 gram of anilinonaphthalene sulfonate, 0.2 gram of di Mix potassium aminonaphthalenesulfonyl chloride and 0.5 g sodium fluorescein.

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Abstract

A fluorescent reagent for diagnosing stomach cancer by spectrophotofluorimetry to measure the content of specific protein in stomach fluid and use of gastroscope and physiological section is preparedfrom one or more of acetic acid, citric acid, phosphoric acid, boric acid and tartaric acid, one or more of particular their salts, and one or more of fluoramine, NBD-Cl, fluorecein sodium, etc through mixing.

Description

technical field [0001] The invention relates to a reagent for measuring the content of specific protein in gastric juice by using fluorescence spectrophotometry when the excitation wavelength is 250-350 nanometers and the emission wavelength is 300-600 nanometers, and cooperates with gastroscope and physiological section to diagnose gastric cancer, especially relates to a A fluorescence diagnostic reagent for gastric cancer. Background technique [0002] Fluorescence diagnosis of tumors is to use ultraviolet light to irradiate the gastric juice of the human body, measure the fluorescence of the gastric juice, and diagnose the nature of human tumors according to the spectral characteristics of the autofluorescence of the human gastric juice. As a common disease, gastric cancer seriously endangers people's health and life safety. At present, in the process of diagnosis and treatment of gastric cancer, it is necessary to use gastroscopy and physiological slices to diagnose gast...

Claims

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Application Information

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IPC IPC(8): G01N33/52
Inventor 李莉
Owner 李莉
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