Recombined fowl pox living carrier vaccine for co-expression of MIV-1CNB and hlL-6 proteins

A technology of HIV-1CNB and live vector vaccine, applied in the field of recombinant fowlpox virus, can solve the problems of long-term or life-long medication, short half-life, high price, etc., and achieve good genetic stability

Inactive Publication Date: 2004-04-21
MILITARY VETERINARY INST MILITARY SUPPIES PLA
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AI Technical Summary

Problems solved by technology

Although AIDS drug chemotherapy is perfected day by day, the following shortcomings are common: it cannot completely treat AIDS, it is expensive, it has high toxicity and side effects, drug-resistant strains will appear after long-term use, and HIV-infected patients and AIDS patients need long-term or life-long medication, etc.
[0008] Cytokines have a stimulating effect on the immune system, but in vitro injection of cytokines, because of their short half-life and rapid degradation, cannot maintain a high level in the body and reach specific target cells, requiring repeated injections, followed by serious side effects
Therefore, the curative effect of in vitro injection of cytokines has certain limitations

Method used

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  • Recombined fowl pox living carrier vaccine for co-expression of MIV-1CNB and hlL-6 proteins
  • Recombined fowl pox living carrier vaccine for co-expression of MIV-1CNB and hlL-6 proteins
  • Recombined fowl pox living carrier vaccine for co-expression of MIV-1CNB and hlL-6 proteins

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Embodiment Construction

[0031] 1. Routine genetic manipulation in molecular biology

[0032] Preparation and transformation of Escherichia coli competent cells, extraction of plasmids and digestion with restriction endonucleases, recovery of DNA fragments, ligation of linear DNA fragments, screening and identification of recombinant plasmids, PCR amplification reactions, etc. The second edition of "Molecular Cloning Experiment Guide", related chapters of Science Press (1992).

[0033] 2. Construction of recombinant expression plasmids

[0034] 2.1 Construction of chimeric gene gag-gp120 intermediate plasmid

[0035] After the pKSGAG plasmid was double-digested with restriction endonucleases Xba I and Bgl II, the HIV-1 gag gene was recovered, and after the pKSGP120 plasmid was double-digested with Xba I and BamH I, a large fragment containing the HIV-1 gp120 gene was recovered. The latter was directionally connected with the gag gene to construct an intermediate plasmid pKSGE containing the chimeric...

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Abstract

A recombinant live-carrier vaccine for henpox is disclosed, which can express the structural protein HIV-1CNB and hIL-6. Three recombinant henpox virus strains vPUTAIL6-GAG, vPUTAIL6-GP and vPUTAIL6-GE can express the HIV-1CNB core protein gag, outer membrane protein gp120 or chimeric protein gap-gp120, and hIL-6. They can stimulate the organ to generate HIV-1 resistant specific homoral immune and cell immune. Its advantages are high safety and high genetic stability.

Description

Technical field: [0001] The present invention provides three strains respectively co-expressing Chinese epidemic strain type I human immunodeficiency virus subtype B (HIV--1 CNB The invention relates to a recombinant fowlpox virus comprising core protein gag, outer membrane protein gp120 or gag-gp120 chimeric protein and cytokine human interleukin 6 (hIL-6), belonging to the field of biotechnology. Background technique: [0002] AIDS (AIDS) is a viral infectious disease caused by human immunodeficiency virus (HIV) infection that is the most serious threat to human beings, the most difficult to control and the largest cost. According to the report of the World Health Organization, AIDS has been prevalent since the early 1980s, and there are about 30 to 40 million infected people in the world, and about 16,000 people are infected with HIV every day. Since the first AIDS patient was discovered in Beijing in 1985, the prevalence of AIDS in my country has entered a period of rap...

Claims

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Application Information

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IPC IPC(8): A61K39/275A61P31/12
Inventor 金宁一江文正李子健张立树金洪涛王宏夏志平张洪勇
Owner MILITARY VETERINARY INST MILITARY SUPPIES PLA
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