Soy protein concentrate having high isoflavone content and process for its manufacture
A technology of soybean protein and isoflavones is applied in the direction of protein composition of vegetable seeds, vegetable protein processing, protein food ingredients, etc., which can solve the problem of not teaching soybean protein and so on.
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Embodiment 1
[0077] Disperse 23kg of soybean flour with a PDI of 86 into 236 kg of water to form a slurry. The pH was adjusted to 7.5 with NaOH. The suspension was stirred at 60°C for 30 minutes and then centrifuged in a decanter centrifuge. The insoluble centrifuge cake was discarded, and the supernatant was heat-treated with a steam heater at 121° C. for 15 seconds. It was then cooled to 48.8°C in a jacketed tank. The suspension was filtered through a spiral membrane with a cut-off molecular weight (MWCO) of 10,000, allowing 75 wt.% of the feed volume to permeate. The resulting retentate was steam heated at 93°C for 15 seconds. The retentate was then cooled to 60°C in a jacketed tank and spray dried. Analyze the product to determine its content.
[0078] The results of the two batches (Table 1) showed that the protein content in the product was 79.79 wt.% and 82.97 wt.% of the total dry matter. The total isoflavones are at least 2 mg / g total dry matter, and the total content of raf...
Embodiment 2
[0096] About 227 g of water is added to the mixing tank and heated to a temperature of about 60°C. Then, add about 45kg soybean cake in mixing tank, become slurry. The pH of the slurry was adjusted to about 7.1 using about 1400 ml of 4.5% NaOH solution. The slurry was mixed for 10 minutes at a temperature of 55-58°C and then transferred to the feed tank of the centrifuge containing about 303 liters of water preheated to about 60°C. The diluted slurry was mixed for 20 minutes at a temperature of 55-58°C and then fed to a Sharples bowl centrifuge at a feed rate of 7.6 liters / minute. The supernatant was steam heated to a temperature of about 127°C. The steam-heated supernatant is transferred to the membrane feed tank through a 100-mesh filter. Add about 10 g of sodium metabisulfite to the membrane feed tank. The supernatant was fed to an ultrafiltration membrane system containing a spiral membrane with MWCO of 10,000. During the membrane process, the temperature of the super...
Embodiment 3
[0124] About 227 g of water is added to the mixing tank and heated to a temperature of about 60°C. Then, add about 45kg soybean cake in mixing tank, become slurry. The pH of the slurry was adjusted to about 7.08 using about 1400 ml of 4.5% NaOH solution. The slurry was mixed for 10 minutes at a temperature of 55-58°C and then transferred to the feed tank of the centrifuge containing about 303 liters of water preheated to about 60°C. The diluted slurry was mixed for 20 minutes at a temperature of 55-58°C and then fed to a Sharples bowl centrifuge at a feed rate of 7.6 liters / minute. The supernatant was steam heated to a temperature of about 127°C. The steam-heated supernatant is transferred to the membrane feed tank through a 100-mesh filter. Add about 10 g of sodium metabisulfite to the membrane feed tank. The supernatant was fed to an ultrafiltration membrane system containing a spiral membrane with MWCO of 10,000. During the membrane process, the temperature of the supe...
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