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Method for extracting rice protein by alkaline process

A rice protein and alkaline extraction technology, which is applied to the preparation method of peptides, chemical instruments and methods, plant peptides, etc., can solve process parameters such as NaOH concentration extraction time, unreasonable solid-liquid ratio, and no alkaline extraction The rice protein industrial production method and alkaline extraction method are difficult to apply to industrial production and other problems, so as to achieve good extraction effect, low cost and low production cost

Inactive Publication Date: 2004-09-15
GAEA GEM RICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows us to produce large amounts (upwards 20 million pounds per year), pure proteins from rice grain quickly while also reducing costs associated with traditional methods like acidic or basic techniques. It achieves this through various technical features including solvent immersion technique, liquid/liquide separation, purification processes, selective addition of different chemicals, controllability over processing conditions, simplifying procedures, making them suitable for both commercial producers' supply chain and home users' demand.

Problems solved by technology

This patented technical problem addressed by this patents relates to finding an efficient way to produce high quality rice protein from rice without causing allergies or harmful bacteria during processing.

Method used

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  • Method for extracting rice protein by alkaline process
  • Method for extracting rice protein by alkaline process

Examples

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Effect test

Embodiment 1

[0037] Get 500 grams of early indica rice, add 2.58 liters of 0.09mol / L NaOH solution, soak for 2 hours and refine to 100 mesh or more and stir for 2 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 5.5 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at a high speed of 4000 rpm for 15 minutes, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. P...

Embodiment 2

[0039] Get 500 grams of early indica rice, add 3 liters of 0.09mol / L NaOH solution, soak for 2.5 hours and grind to more than 100 mesh and stir for 2.5 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 5.0 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at 4000 rpm for 15 minutes at high speed, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. P...

Embodiment 3

[0041] Get 500 grams of early indica rice, add 3.5 liters of 0.09mol / L NaOH solution, soak for 3 hours and grind to more than 100 mesh and stir for 3 hours to keep the solution pH=11. Centrifuge at a high speed of 4000 rpm for 15 minutes in a centrifuge to collect the protein supernatant and starch precipitate. Add 0.09 mol / L hydrochloric acid to the protein supernatant, and measure the pH to 4.5 with an acidity meter while stirring while adding. Add 0.09mol / L NaOH solution to the precipitate after acid precipitation to make it neutral, then centrifuge at 4000 rpm for 15 minutes at high speed, discard the supernatant, and collect the protein precipitate. The precipitation is washed with water and then the centrifugation is repeated to obtain rice protein. Dilute the starch precipitate with water and stir evenly, then centrifuge to take the supernatant, repeat the steps of acid precipitation and centrifugation, and part of the rice protein and rice starch can be obtained. Pre...

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Abstract

The invention discloses a method to rice protein by alkali, including the technical step in sequence: rice washing, lye soaking, slurry milling, stir-extracting, separating, acid precipitation, separating and drying; NaOH solution soaking: concentration 0.05-0.15mol/L, solid-liquid ratio 1 to 6-8 and soaking time for 2-3 hours; the stir-extracting is to stir by keeping mixed liquor pH 11, the acid precipitation is isoelectric precipitation, i.e. it adjusts pH value by HCl to 5.5 to precipitate protein; the drying temperature is limited below 50 deg.C. It can continuously extract rice protein on a large scale. Its extraction rate is high and its fat content is low.

Description

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Claims

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Application Information

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Owner GAEA GEM RICE
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