Introducing soybean total DNA to improve nutrient quality of barley grain

Abstract

The method of producing barley seed with raised protein content includes the following steps: providing soybean total DNA and barley seed; introducing soybean total DNA with gene gun into barley seed; growing barley with the seed obtained in the last step and gathering the next generation seed; and identifying the next generation seed with raised protein content. The present invention also provides feed containing the barley seed. The first generation seed thus obtained has greatly raised protein content and unchanged other characteristics.

Description

technical field [0001] The present invention relates to the field of crop breeding, and more particularly, the present invention relates to a method of producing barley seeds with increased protein content, and feedstuffs containing said barley seeds. Background technique [0002] Barley is a widely distributed crop, and its total area and total output rank fourth among all kinds of crops in the world. In my country, barley is also an important crop, and more than 70% of it is used for feed processing. The protein content and amino acid content of barley seeds used as feed are important indicators to measure the quality of barley. It is reported that the protein content of common barley seeds is low, and the amino acid components are unbalanced, especially the average content of the first limiting amino acid lysine is only 0.47% (Sun Guihua et al., Liaoning Agricultural Science, 1996, 3: 38-41; Gui Lianqi et al., Food and Oils, 2001, 2: 40-42). According to the experiment...

AI Technical Summary

Problems solved by technology

Although conventional breeding methods have made some progress in improving crop seed proteins, such as high-lysine cereals and barley mutants, it is well known that using purely traditional cross-breeding often takes a long time to obtain stable progeny (more than 9-10 generations of reproduction), and the increase in protein content is not high. At the same time, most of the high-protein barley selected by conventional breeding has bad traits such as low yield, poor insect resistance and disease resistance. There is often an unfavorable negative correlation between the yield and quality of breeding barley, making them difficult to be popularized and applied in agricultural production (Nelson O E, Proc. of a panel meeting organized by FAO / IAEA, Sweden, 1968; Zhu Muyuan et al., Barley Breeding and Bioengineering, Shanghai Science and Technology Press, 1999, p127)

[0004] In the 1990s, there was a report (Wu Yaowu, Journal of Northwest Agricultural Science, 1994, 3 (1): 9-12) trying to carry out the fusion of pea and barley protoplasts, but the report only described how to improve the fusion frequency of protoplasts of two species Conditions, so far there is no report of successful regeneration of offspring plants

The use of biolistic introduction of total DNA to improve crop quality has never been attempted, either in barley, or in any other crop improvement research.