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Adenosme generation bacteria and method for generating adenosine by fermentation

A technology for producing bacteria and adenosine, which is applied in the direction of fermentation and bacteria, and can solve the problems of limited production and high production cost of adenosine

Inactive Publication Date: 2005-08-24
江苏省苏微微生物研究有限公司 +2
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Problems solved by technology

If the raw material of adenosine is obtained from degraded RNA for the production of ATP, the production cost of adenosine is very expensive, thus limiting the domestic production of ATP

Method used

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  • Adenosme generation bacteria and method for generating adenosine by fermentation

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Embodiment Construction

[0011] 1. Preparation of high-yield adenosine-producing bacteria

[0012] Using the inosine-producing strain JSIM-1019 bred in our laboratory as the starting strain, the parent strain was subjected to mutagenesis treatment with physical and chemical mutagens, the mutagens being ultraviolet light, ethyl methylsulfonate, nitrous Carry out mutagenic breeding for the production of inosine by guanidine, the ultraviolet irradiation time is 0.5-3min, the concentration of ethyl methyl sulfonate is 0.5%-2%, the treatment time is 15-45min, and the concentration of nitrosoguanidine is 300μg / ml- 500μg / ml, the treatment time is 30-50min, the treatment method is continuous or interphase, a series of mutants are obtained, and then the genetic performance of the mutants is additively selected, and a batch of xanthine auxotrophic mutants are obtained. The xanthine auxotrophic mutant strain was inoculated in the seed medium, cultured at 34°C for 18 hours, and then inoculated into the fermentati...

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Abstract

An adenosine generating bacterium Bacillus subtilis JSIM-1025 is disclosed. A fermenting method for generating adenosine with high output (more than 23g / L) features that the fermentation is conducted at 37 deg.C for 60 hr in such conditions that the optional concentration of the ferment cream as the optical component of culture medium is 1.8%, the optimal pH value is 6.5, and the optimal oxygen solubility is 40%.

Description

technical field [0001] The present invention relates to a novel microbial strain and a fermentation method for producing its metabolites using the strain. Specifically, the present invention relates to a high-yield adenosine-producing bacterium, and also relates to a fermentation method for producing adenosine, which utilizes optimal medium components, optimal pH and dissolved oxygen control ranges for the production of adenosine. High yield of adenosine was obtained under the fermentation process conditions. Background technique [0002] adenosine [2] , is the medicine of human circulatory system disease. Because adenosine has the function of promoting coronary dilation and myocardial metabolism, dilating blood vessels, and increasing coronary blood volume, it can be used for the treatment of coronary vascular disorders, angina, arteriosclerosis and hypertension. In addition, adenosine and its derivatives can be used as drugs to inhibit blood coagulation, and the effect ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12P19/38
Inventor 柏建新邓崇亮朱晓宏张一平杜郭君王红连
Owner 江苏省苏微微生物研究有限公司
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