Oxidation resistant bioactive peptide of halobios and preparation method

A technology of antioxidant active peptides and marine organisms, applied in the field of biological peptides, can solve problems such as blanks

Inactive Publication Date: 2005-08-31
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is still blank in terms of isolating and purifying antioxidant active peptides from marine animal shellfish.

Method used

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  • Oxidation resistant bioactive peptide of halobios and preparation method
  • Oxidation resistant bioactive peptide of halobios and preparation method
  • Oxidation resistant bioactive peptide of halobios and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Preparation of antioxidant active protein

[0079] (1) Mince 600 grams of fresh scallop viscera, then add 1.0% NaCl solution (containing 0.2% Triton-100; 0.1mmol / L EDTA) according to the wet weight 1:1 (w / w) and homogenize the tissue Homogenized in a machine. Adjust the pH to 5.6, heat in a water bath at 70°C for 5 minutes, and cool down rapidly. The cooling liquid was centrifuged at 15000rpm for 10min, the supernatant was collected, and the pH was adjusted to 6.7 to collect 60-90% saturated (NH 4 ) 2 SO 4For precipitation, the precipitate was dissolved with 5 mmol / L Tris-HCl (pH7.5) buffer solution and dialyzed. Take the supernatant from the centrifugal dialysate, and slowly add 1.5 times pre-cooled acetone. The acetone precipitate was redissolved in 5 mmol / L Tris-HCl (pH 7.5) buffer solution and fully dialyzed to obtain a crude protein solution, which was lyophilized and stored (sample I).

[0080] (2) The crude protein solution was concentrated with polyethylen...

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Abstract

An antioxidizing peptide for marine biota is a micro-molecular polypeptide consisting of 7 amino acid and containing 8 amino acid residues. Its preparing process is also disclosed. Its advantages are strong antioxidizing activity, high water solubility, and no poison and by-effect. It is suitable for food, cosmetics, medicines, etc.

Description

technical field [0001] The invention relates to the field of biological peptides, in particular to marine biological antioxidant active peptides extracted from marine animal shellfish and a preparation method thereof. Background technique [0002] As we all know, marine biological products are an important source of protein in people's daily life, which shows that marine products contain a variety of rich proteins. According to reports, proteins with antioxidant activity from marine shellfish are a class of proteins containing acidic heteropolysaccharides and have strong heat resistance. The viscera of marine shellfish also contains a large amount of glycogen and other polysaccharides, as well as rich in protein. In the past, salting out and acetone precipitation were often used to separate the polysaccharide components from the extract. [0003] As post-processing products of proteins, enzymatically active polypeptides are mostly small molecule polypeptides with the same n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K7/06C12P21/02
Inventor 王跃军孙谧邹建王海英
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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