Perivascular wraps
A biological, polymer technology, applied in the direction of blood vessels, cardiovascular system diseases, fabrics, etc.
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Embodiment 1
[0182] Synthesis of Polymer MEPEG750-PDLLA-2080 Polymer
[0183] To synthesize the MePEG750-PDLLA-2080 polymer, weigh 40 g of MePEG (molecular weight = 750; Sigma-Aldrich, St. Louis, MO) in a 500RB flask, and 160 g of D,L-lactide (PURASORB , PURAC, Lincolnshire, IL). Both reagents were dried under vacuum overnight at room temperature. 600 mg of stannous 2-ethyl-hexanoate catalyst (Sigma) was then added to the RB flask containing MePEG and a magnetic stir bar. Flask with N 2 Purge (without oxygen) for 5 minutes, cover with a glass stopper, place in an oil bath (maintained at 135°C), and gradually adjust the magnetic stirrer to setting 6 (Corning). After 30 minutes, the flask was removed from the oil bath and cooled to room temperature in a water bath. Add D,L-lactide to the flask, and then 2After purging for 15 minutes, the flask was capped and placed in an oil bath (135° C.) again. Adjust the magnetic stirrer to setting 3 and allow the polymerization to continue for at...
Embodiment 2
[0185] Purification of MEPEG750-PDLLA-2080
[0186] MePEG750-PDLLA-2080 was prepared as outlined in the example, then 75 g of MePEG750-PDLLA-2080 was dissolved in 100 ml of ethyl acetate (Fisher, HPLC grade) in a 250 ml Erlenmeyer flask. The polymer was precipitated by slowly adding the solution to 900 ml of isopropanol (Caledon, HPLC grade) in a 2L Erlenmeyer flask with stirring. The solution was stirred for 30 minutes and the suspension was cooled to 5°C using a cooling system. The supernatant was separated and the pellet was transferred to a 400ml beaker. The polymer was first pre-dried in a forced air oven at 50°C for 24 hours to remove most of the solvent. The pre-dried polymer was then transferred to a vacuum oven (50°C) and further dried for 24 hours until residual solvent was below acceptable levels. Store the purified polymer at 2-8°C.
Embodiment 3
[0188] Coat MEPEG750-PDLLA-2080 on PLGA (10:90) mesh
[0189] Wash with isopropanol (Caledon, HPLC) and measure 3 x 6 cm 2 PLGA (10:90) mesh and dried in a forced air oven at 50°C. 3 g of MePEG750-PDLLA-2080 were then dissolved in 15 ml of ethyl acetate (20% solution; Fisher HPLC grade) in a 20 mL glass scintillation vial. Paclitaxel (10.13 mg) was added to the polymer solution and paclitaxel was completely dissolved by using a vortex mixer. The meshes were coated with the polymer / paclitaxel solution by dipping into the solution. Excess solution was then removed and the coated mesh was dried using an electric fan for 2-3 minutes. The coated mesh was placed in a PTFE petri dish and dried further in a fume hood using an electric fan for 60 minutes. The coated mesh was then transferred to a vacuum oven and dried overnight at room temperature under vacuum. The dry coated mesh was packed between two pieces of release-liner (Rexam A10) and sealed in the pouch.
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