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Medicine for treating and preventing tumour, and its prepn. method

A preparation and tumor technology, which is applied in the field of preparations for the prevention and treatment of tumors, can solve the problems of low tumor inhibition rate, complex components, and increase the difficulty of developing drugs, and achieve high anticancer activity, broad anticancer spectrum and unique performance Effect

Inactive Publication Date: 2006-01-11
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In the above-mentioned prior art, photosynthetic bacteria are also used to transform Chinese herbal medicines into reports for the preparation of medicines for treating cancer. There are great difficulties in terms of time and funds, not to mention the transformation with photosynthetic bacteria, the composition will be more complex, which increases the difficulty of developing into drugs, and there is no chemical composition description in its patent report, and its tumor inhibition rate in animal experiments lower

Method used

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  • Medicine for treating and preventing tumour, and its prepn. method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Use the following photosynthetic bacteria culture medium, the formula is: sodium acetate 1500mg, CaCl 2 .2H 2 O 75mg, MgSO 4 .7H 2 O 200mg, EDTA 20mg, yeast extract 1000mg, K 2 HPO 4 1000mg, (NH 4 ) 2 SO 4 1500mg, KH 2 PO 4 700mg, FeSO 4 .7H 2 O 10mg, trace element solution 4ml, deionized water 1500ml. PH is 7.

[0050] The composition and content of the trace element solution are: H 3 BO 3 250mg, Na 2 MoO 4 .2H 2 O 75mg, CuSO 4 5mg, MnSO 4 .4H 2 O 200mg, ZnSO 4 .7H 2 O 25mg, 150ml deionized water.

[0051] Add 50ml of Rhodopseudomonas palustris photosynthetic bacteria species into 500ml photosynthetic bacteria culture medium, culture for 10 days under 1000 Lux light and anaerobic conditions, as the photosynthetic bacteria culture solution.

[0052] The stems and branches with leaves of Viscum coloratum (Kom.) Nakai of mistletoe are dried by air and crushed. Divide 125g of crushed mistletoe powder into 1800ml of 40% ethanol three times, extract for 1 hour each time, combine...

Embodiment 2

[0053] Example 2: Preparation of photosynthetic bacteria culture solution. The photosynthetic bacteria strains were mixed strains of Rhodopseudomonas viridis and Rhodobacter sphaeroides, and the others were the same as in Example 1. The stems and branches of Ginkgo mistletoe V.album.L. , After natural air drying, crushed, 125g of crushed mistletoe powder was extracted with 3500ml of 95% ethanol in 4 times, each extraction was 30 minutes, the filtrates were combined, and the ethanol in the extract was recovered under reduced pressure and concentrated to 150ml. Dilute to 1200ml, adjust pH to 6, sterilize at 121℃ for 20min to obtain mistletoe medium mixture; inoculate 250ml of photosynthetic bacteria culture into the above mistletoe medium mixture, and cultivate 10 under 2000 Lux light and anaerobic conditions Sterilize at 121°C for 40 minutes to prepare sterilized mistletoe transformed by photosynthetic bacteria.

Embodiment 3

[0054] Example 3: Preparation of photosynthetic bacteria culture solution. The photosynthetic bacteria were a mixture of Rhodospirillum cristatum, Rhodopseudomonas viridis, and Rhodopseudomonas marine bacteria. Others were the same as in Example 1; The stems and branches with leaves of V. diospyrosicolum Hayata are air-dried and crushed. The crushed mistletoe powder 125g is divided into 1500ml of 80% methanol for 2 times, each extraction is 4 hours, the filtrate is combined, and the methanol in the extract is recovered under reduced pressure , Concentrated to 200ml, diluted to 3000ml with culture medium, adjusted pH to 8, and sterilized at 121℃ for 30min to obtain mistletoe medium mixture; 100ml photosynthetic bacteria culture medium was inoculated into the above mistletoe medium mixture, and lighted at 500 Lux , Under anaerobic conditions, cultivated for 30 days, it is made into the preparation of photosynthetic bacteria into the living bacteria of mistletoe.

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Abstract

A medicine for preventing and treating tumor is prepared through preparing the culture liquid of photosynthetic bacteria, preparing the liquid extract of colored mistletoe, proportionally mixing them, culturing for 3-30 days under illuminescence and anaeration condition to obtain the living bacteria preparation, and sterilizing.

Description

1. Technical field [0001] The present invention relates to a preparation for preventing and treating tumors, specifically a live bacteria preparation or a sterilization preparation made by adding mistletoe extract under photosynthetic bacterial culture conditions through mixed fermentation, as well as the preparation method and use of the preparation. 2. Background technology [0002] Photosynthetic bacteria (PSB) are the earliest prokaryotic ancient microorganisms that appeared on the earth 2 billion years ago and use solar energy to grow and reproduce through photosynthesis. They can perform photosynthesis without releasing oxygen and play a role in the natural C, N, S, P and other cycles. important role. In the past, it was mostly used in the treatment of organic wastewater. In recent years, the nutritional value and some special physiological activities of bacteria have been gradually recognized, and its research and development has attracted increasing attention from co...

Claims

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Application Information

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IPC IPC(8): A61K36/126A61P35/00
Inventor 杨官娥张肇铭
Owner SHANXI UNIV
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