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Cellular preparation

A cell preparation and cell technology, applied in the field of cell preparation, can solve problems such as the lack of established application technology

Inactive Publication Date: 2006-04-26
井上 一知 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, although PVA is a high molecular polymer suitable for this microcapsule type bioartificial organ due to its characteristics, its application technology has not yet been established.
Therefore, there is no bioartificial organ that utilizes the beneficial properties of PVA as in the present invention and in which cells can be stably retained within the PVA gel

Method used

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  • Cellular preparation
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Examples

Experimental program
Comparison scheme
Effect test

manufacture Embodiment 1

[0098] (Manufacturing Example 1) Preparation of sheet-like cell preparation containing islet cells

[0099] (1) Preparation of islet cells

[0100] Islet cells were isolated from Wister rats (Shimadzu animal Co. Ltd. Kyoto, Japan) aged 8 to 10 weeks and weighing 300 to 350 g by the following method.

[0101] First, 10 ml of collagenase type I (Sigma, St.Louis, MO, USA, 350 U / mg) and collagenase type XI (Sigma, St.Louis, MO, MO, 2,200 U / mg) 800 U / ml and 1500U / ml of Hank's solution was injected into the pancreatic ducts of rats respectively, and the pancreas was extracted and enzymatically treated at 37°C for 15 to 20 minutes. The enzymatic reaction was terminated by adding cold Hank's solution. Pancreatic tissue was pipetted and centrifuged to recover the tissue pellet, which was then rinsed twice with Hank's solution. Centrifuge at 1,000 rpm for 1 minute. The tissue suspension was filtered through an 800 [mu]m size mesh sheet. The filtrate was recovered into a 50 ml conical...

manufacture Embodiment 2

[0122] (Manufacturing Example 2) Preparation of sheet-like cell preparation containing islet cells

[0123] (1) Preparation of islet cells

[0124] Wister rats were general anesthetized by inhalation of diethyl ether and intraperitoneal administration of 1 mg / kg pentobarbital (Nembutal). After incision, the common bile duct is identified and a tuft is formed near the Vater's mastoid with non-toothed forceps. A small opening was formed at the junction of the cystic duct and the cannula was inserted into the common bile duct, fastened and fixed, and injected with collagenase type XI (Sigma, St.Louis, MO, USA) equivalent to 1200 to 1500u / ml. ) to dilate the pancreas. Without crushing, the whole pancreas was extracted, placed in a Erlenmeyer flask, immersed in a constant temperature bath at 37°C and degraded for about 18 minutes. After degradation, shake the flask several times to break up the pancreas and rinse three times with Hank's solution containing 0.1% bovine serum albu...

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PUM

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Abstract

The present invention provides cell preparations, cells that can be used in patients to secrete biologically active factors such as hormones or proteins can be stably maintained in polyvinyl alcohol for a long time. Preparations for the prevention and treatment of endocrine / metabolic diseases, hemophilia, bone diseases or cancer.

Description

technical field [0001] The present invention relates to cell preparations that can be used in human or animal pharmaceutical compositions. In particular, the present invention relates to cell preparations in which cells can produce / secrete biologically active factors such as hormones and proteins usable in living bodies, or cells can detoxify harmful substances that can be stably retained for a sustained period of time, thereby showing that by administering Efficacy of preventing / treating endocrine / metabolic disease, hemophilia, bone disease or cancer in patients. Background technique [0002] Bioartificial organs are devices that contain living cells and biological tissues and provide metabolic functions required by patients, more specifically, biologically active factors that regulate metabolic functions of patients, such as hormones and proteins, to prevent or treat patients' diseases. Compared with living donor organ transplantation, which has the side effects of immuno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61K35/12A61K38/39
CPCA61K38/39A61L27/38A61K35/12A61K35/24
Inventor 井上一知顾元骏角昭一郎
Owner 井上 一知
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