Anti-cancer active protein of syngnathus

An anti-tumor activity and active protein technology, applied in the field of sea dragon anti-tumor active protein, to achieve the effect of enhancing immunity and improving signs and traits

Inactive Publication Date: 2006-06-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the whole body is still used as medicine for the treatment of bruises and impotence and nocturnal

Method used

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  • Anti-cancer active protein of syngnathus

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Experimental program
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Effect test

Embodiment 1

[0019] The anti-tumor active protein provided by the invention is white powder in appearance, the protein content is 99.5% detected by the Lowry method, and the sugar content is 0.5% detected by the phenol-concentrated sulfuric acid method.

[0020] SDS-PAGE was performed on the protein, the concentration of the separating gel was 12%, the concentration of the stacking gel was 5%, and the mixed standard protein with a molecular weight of 14.4-116kDa was used as a control, the results are shown in figure 1 , wherein Marker: protein molecular weight standard; Sample: sea dragon anti-tumor active protein in the present invention. Depend on figure 1 It can be seen that the anti-tumor active protein of sea dragon provided by the present invention shows a single band in SDS-PAGE, with a molecular weight of 55kDa.

[0021] The protein was tested for its anti-tumor activity. It was detected by the MTT method. When the drug concentration was 100 μg / ml, it was effective against Lovo (h...

Embodiment 2

[0024] The anti-tumor active protein provided by the present invention is white powder in appearance, the protein content is 100% detected by the Lowry method, and the sugar content is 0% detected by the phenol-concentrated sulfuric acid method; SDS-PAGE shows a single band with a molecular weight of 55kDa .

[0025] The in vivo activity of this protein was investigated by animal transplantation tumor experiment method: 10 ICR mice (42 ± 2 days old, body weight 18-22g, female) bearing S180 sarcoma were injected with 20mg / The dose of Kg day is given by intraperitoneal injection for seven consecutive days; positive control is 10 tumor-bearing mice (75mg / kg, administered twice in 1 and 4 days after tumor inoculation) for intravenous injection of 5-fluorouracil; negative control is not 10 tumor-bearing mice were administered. After the mice were weighed on the 10th day, the tumors of each mouse were dissected and weighed, and their immune indexes were measured at the same time. ...

Embodiment 3

[0030] The anti-tumor active protein provided by the present invention is white powder in appearance, the protein content is 99% detected by the Lowry method, and the sugar content is 1% detected by the phenol-concentrated sulfuric acid method; SDS-PAGE shows a single band with a molecular weight of 55kDa .

[0031] The in vivo activity of the protein was investigated by the animal transplantation tumor experiment method, and the experiment method was the same as that in Example 2. When the dosage is 30 mg / Kg·day, the tumor inhibition rate to mice bearing S180 sarcoma is 51.14%.

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Abstract

The invention relates to seadragon anticancer active albumen that uses thick seadragon as raw material to distil active albumen that has molecular weight of 55kDa. The albumen content is 99-100%, and sugar content is 0-1%. The appearance is white powder. SDS-PAGE shows a single banding. The invention has anticancer function and has the feature of low toxin, enhancing immunity. It could be used in curing or assistant to cure kinds of cancer.

Description

technical field [0001] The invention relates to a product in the technical field of medicine, in particular to a sea dragon anti-tumor active protein. Background technique [0002] At present, there are many anti-tumor drugs used in clinic at home and abroad, and the toxic and side effects of chemically synthesized drugs are relatively large. The separation and purification of drugs with anti-tumor and immune-enhancing activities from natural products has become a research hotspot. In recent years, the research on active proteins and peptides in animals has become increasingly active, and finding anti-tumor proteins and peptides has become a new hotspot in research and development. Due to the differences between marine organisms and inland organisms, protein components from marine animals have more development and application value. At present, hundreds of marine biological peptides and proteins have entered clinical research in the world. The RNase-Onconase (P-30 protein, ...

Claims

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Application Information

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IPC IPC(8): C07K14/435A61P35/00
Inventor 李晓波张朝晖秦孙星王威王梦月
Owner SHANGHAI JIAO TONG UNIV
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